Biomedical subjects

G A Iakovleva

Publications and source records attributed to G A Iakovleva.

3 recordsLinked to original sources

PubMedDate not supplied

[Induced phytophthora resistance in transgenic potato tubers].

Resistance of transgenic cultivars based on the expression of one or more resistance genes is sooner or later broken by pathogens whose race-producing rates are high. Thus, combining transgenesis with elicitor-induced resistance is a promising approach. The elicitor-induced resistance is based on the expression of multiple resistance genes, which can prevent the adaptation of pathogens to transgenic races, maintain the stability of cultivars, and increase their lifespan. In this work, we used transgenic potato cultivars Temp and Superior transformed with Bacillus thuringiensis delta-endotoxin gene and Lukyanovskii transformed with leukocyte alpha-interferon gene. Arachidonic acid (10(-8) M) and soluble chitosan (5 kDa, 100 micrograms/ml) were used as elicitors for tuber treatment. Our data showed that pretreatment with elicitors causes a 15-25% increase in both the systemic prolonged resistance of potato tubers to Phytophthora infestans and their ability to repair mechanical damage.

Arachidonic Acid↗

PubMedDate not supplied

[A pH-probe for endoscopic parietal topographical pH-metry].

A pH-probe for endoscopic parietal topographical pH-metry was developed. The probe has a circular measuring electrode and a cutaneous silver chloride reference electrode. Clinical testing of the developed probe was performed to compare it with a conventional end electrode. It was found that the new pH-probe with circular antimonial electrode for pH measurement at the surface of the mucous coat of upper gastrointestinal tract in a fasting patient considerably increases the accuracy of pH measurement at the site of acid glands. Recommendations for examination of acid-forming and alkalizing functions of stomach and determination of the boundaries of the main regions of stomach during endoscopic examination are given.

Electrodes↗

PubMedDate not supplied

[Expression of a partially modified delta-endotoxin gene from Bacillus thuringiensis var. tenebrionis in transgenic potato plants].

A modified gene (Bt77) of delta-endotoxin from Bacillus thuringiensis var. tenebrionis was constructed and cloned into pMON505. This binary transformation vector was introduced into Agrobacterium tumefaciens strains containing different helper disarmed Ti-plasmids, LBA4404, A281, and CBE21. These Agrobacterium strains were used to transform potato stem segments (S. tuberosum, cv Desiree, Resy, Temp, Granat). Regenerants were selected on kanamycin-containing media. The presence of the Bt77 sequence in plant genomic DNA was confirmed by PCR analysis. Bt gene expression was studied in regenerated plants. Western blot analysis revealed that transgenic plants produced the Bt protein in the range of 0.005-0.02% of total protein. Total protection against insect damage of leaf tissue from these plants was observed in laboratory bioassays with of Colorado beetle larvae. Transgenic plants showed incomplete protection from CB larvae.

Agrobacterium tumefaciens↗