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G A Koroleva

Publications and source records attributed to G A Koroleva.

At least 19 recordsLinked to original sources
PubMed2004

Recombination in uveitis-causing enterovirus strains.

The complete nucleotide sequences of three human echovirus (EV) 11 strains and one EV19 strain, all of which caused outbreaks of enterovirus uveitis (EU), a new infant disease first identified in 1980 in Siberia, were determined. One EV11 strain which caused an outbreak of sepsis-like disease in Hungary was also sequenced. All four EV11 strains were mosaic recombinants of the prototype EV11 strain Gregory, with their non-structural coding regions and 5' NTRs being more similar to other prototype enteroviruses (EV1, EV9). However, this finding is probably a feature of all circulating enterovirus strains and may not be related to their altered virulence. A full genome sequence comparison of the three subtypes of EU-causing strains excludes the role of recent recombination in their emergence, and points to their independent emergence.

5' Untranslated Regions↗
PubMed2003

Molecular epidemiology of enteroviruses causing uveitis and multisystem hemorrhagic disease of infants.

We studied molecular epidemiology of highly virulent echovirus 11 and 19 strains that were isolated during five outbreaks of enterovirus uveitis (EU) in Siberia in 1980-1989, and three outbreaks of multisystem hemorrhagic disease of infants (MHD) in 1988-1991. Three genome regions, 5'NTR, VP1-2A junction, and a fragment of 3D polymerase, were analyzed. Phylogenetic grouping in the VP1-2A region correlated with serotyping results. All studied EV11 and EV19 strains, including the prototype EV11 and EV19, formed a major phylogenetic group in VP1-2A region. Within that group, several EV11 isolates from EU and MHD outbreaks formed a distinct cluster in VP1-2A and 5' NTR genome regions, designated EV11/B. All strains of this cluster possessed high virulence for monkeys compared with the prototype echoviruses. Subgrouping within this cluster correlated with year of virus isolation, not with the disease the viruses caused in infants (EU or MHD).

Base Sequence↗
PubMed2002

Phylogenetic and serological characterization of echovirus 11 and echovirus 19 strains causing uveitis.

The strains of echovirus 19 (EV 19) and echovirus 11 (EV11), isolated from infants with similar clinical symptoms of acute enterovirus uveitis (EU) in Russia (Siberia) in 1980-1989, were investigated phylogenetically (nucleotide sequence of a 300 nt fragment in 5' NTR and VP4 junction) and serologically. The result confirmed that viruses belong to the Enterovirus genus, with 58-80% nt sequence homology with previously sequenced enteroviruses, and showed the genetical identity between the strains isolated during each of five outbreaks of the EU. The results also demonstrated that isolates from the last three outbreaks of EU belong to the same phylogenetic group despite the remarkable spatial and temporal distance between the outbreaks. The results confirm the role of these echoviruses in the etiology of the EU. Based on phylogenetic and serological comparisons the studied strains were divided into three distinct groups: group I, EV19/K (Krasnoyarsk, 1980-1981), group II, EV11/A (Krasnoyarsk, 1982), group III, EV11/B (Krasnoyarsk, 1986; Omsk, 1987-1988; Irkutsk, 1989). Minor details of the epidemiology of the outbreaks were also revealed.

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PubMed1990

[The determination of free hemoglobin in blood plasma using the hemiglobincyanide method].

The authors have proved the possibility of using the hemiglobincyanide method for measurements of blood plasma free hemoglobin: free hemoglobin was measured in autoblood reinfused from the operation field by various methods (45-benzidine method, 3 parallel analyses in every study, and 45-hemiglobincyanide method, modified by increasing the amount of the tested material from 0.02 to 2.0 ml, also 3 parallel analyses per sample); a correction coefficient equal to 71.7 is suggested, and correction of the data according to a formula.

Blood Transfusion, Autologous↗
PubMed1986

[Characteristics of human-mouse hybrid cell lines and the changes induced in them by the poliomyelitis virus].

Cells of the four hybrid lines between continuous mouse cells Rag and human diploid embryonal fibroblasts were polymorphic and had mitotic activity in fully formed monolayers. Most of the these mitoses were pathological. Hybrid cells examined 8 months after hybridization were susceptible to the poliomyelitis virus infection with partial cytopathologic effect, they produced virus antigens and the infectious virus. Small hybrid cells displayed a more pronounced cytopathologic effect than did big, polynuclear and mitotic cells. Hybrid cells that were passaged 1.5 months after infection did not excrete any infectious poliovirus but contained poliovirus antigens.

Animals↗
PubMed1977

Differences in multiplication of virulent and vaccine strains of poliovirus type I, II, and III in laboratory animals.

Multiplication of virulent and vaccine strains of poliovirus type I, II and III in laboratory animals of different species was studied comparatively. The main criterion of virus reproduction was the production of the photoresistant virus progeny after inoculation of the animals with proflavin-photosensitized virus strains. On the whole, virulent poliovirus strains were characterized by replication in a wide range of hosts (monkeys, cotton rats, white mice, guinea pigs, rabbits, chickens, chick embryos), a low infective dose, production of the photoresistant progeny to a high titre, clinically overt disease in some animal species. The vaccine strains multiplied in a norrower range of hosts, had a high infective dose, a low titre of virus progeny, and caused no clinical symptoms of infection. These differences may serve as a marker for differentiation between virulent and attenuated strains in vivo. Administration of guanidine before inoculation of newborn cotton rats completely prevented or delayed by several days the production of photoresistant virus progeny. This fact confirms the stability of the proflavin-poliovirus complex under conditions ruling out virus replication.

Animals↗
PubMed1976

The morphology of tumors of the human gastrointestinal tract in short-term organ culture and the reaction of these tumors to infection with poliovirus.

A morphologic study of 57 cancers of the human gastrointestinal tract maintained in short-term organ culture was carried out, and the response of part of these tumors to infection with poliovirus was studied. The tumors, grown in vitro, mainly retained their original characteristics and histologic types. A certain correlation between the histologic type and its viability in short-term organ culture was revealed: the more differentiated forms were maintained better than the poorly differentiated ones. A comparative study of the polio-virus-infected and the control cultures indicated that wild poliovirus showed the most marked oncolytic activity in short-term organ culture, whereas attenuated poliovirus III produced a less marked, although still distinct, destructive effect.

Adenocarcinoma↗
PubMedDate not supplied

[Acute enterovirus uveitis in infants].

Enterovirus uveitis (EU) is a new infant eye disease that was first detected and identified in Russia in 1980-1981. Three subtypes of human echoviruses (EV19K, EV11A, and EV11/B) caused 5 nosocomial outbreaks of EU in different Siberian cities and towns in 1980-1989, by affecting more than 750 children mainly below one year of age. Sporadic and focal EU cases (more than 200) were also retrospectively diagnosed in other regions of Russia and in different countries of the former Soviet Union. There were following clinical manifestations: common symptoms of the infection; acute uveitis (rapid focal iridic destruction, pupillary deformities, formation of membranes in the anterior chamber of the eye); and in 15-30% of cases severe complications, cataract, glaucoma, vision impairments. Uveitis strains EV19 and EV11 caused significant uveitis in primates after inoculation into the anterior chamber of the eye, as well as sepsis-like fatal disease with liver necrosis after venous infection. The uveitis strains are phylogenetically and pathogenetically close for primates to strains EV19 and EV11 isolated from young children with sepsis-like disease. The contents of this review have been published in the Reviews in Medical Virology, 2004, vol. 14, p. 241-254.

Animals↗
PubMedDate not supplied

[The CNS lesion in monkeys spinally infected with the vaccinal poliomyelitis virus].

Specific features of the CNS lesions in monkeys inoculated with a vaccine poliovirus strain were studied in comparison with those developing after inoculation with virulent strains. The lesions in the lumbar cord and other regions of the brain and spinal cord after intraspinal inoculation of the vaccine virus are described at length. Certain signs reflecting the specific nature of the lesions were observed. The results of histopathological and immunofluorescent studies were shown to be important for differentiation of specific and nonspecific lesions.

Animals↗
PubMedDate not supplied

[Development of poliovirus infection in laboratory animals of different species].

The capacity of vaccine and virulent strains of poliomyelitis virus to multiply in laboratory animals of different species was studied. Virus reproduction was judged by formation of photoresistant virus progeny in response to inoculation of the animals with photosensitized virus. Multiplication of virulent poliomyelitis virus strains observed in the majority of animal species examined (monkeys, newborn and adult cotton rats, newborn and adult white mice, chickens, chick embryos) resulted in active formation of photoresistant virus population and in some cases was accompanied by clinical symptoms of the disease. Multiplication of vaccine strains was observed in a smaller number of animal species and was limited, as a rule. Among non-primate animals, newborn cotton rats were most susceptible to poliovirus infection. Newborn guinea pigs were the only species of laboratory animals in which no multiplication of any of the six strains under study could be detected.

Animals↗
PubMedDate not supplied

[Role of enterovirus infection in the etiology of uveitis in children].

A total of 224 cases with endogenic uveitis in infants are analyzed. The results of complex clinical and immunological analysis and serologic findings bring the authors to a conclusion that enteroviral uveitis makes up 42 percent of all uveitis in children aged under 3 and that this condition is prevalent in this country. Typical late complications, long persistence of virus-neutralizing antibodies in high titers (up to 8-10 years after convalescence) permit a retrospective etiological diagnosis.

Enterovirus Infections↗
PubMedDate not supplied

[Uveitis, caused by Echo-11 virus].

Analysis of clinical manifestations of a total enteroviral infection with ECHO-11 virus-induced uveitis in 58 infants aged 2 weeks to 1.5 years has shown that uveitis develops on days 1-10 of the disease and is associated with a weak injection, endothelial edema, hyperemia and edema of the iris rapidly eventuating in destruction of the pigment lamina and fenestration. Posterior synechiae, pupil deformations, grave uveitis with hypotonia of 4-10 mm Hg are rapidly developing. A two-year follow-up has shown marked hemo- and hydrodynamic disorders, retarded growth of the involved eye, development of grave complications in 60 percent of patients. The most characteristic late complications were iridal fenestration, pseudocoloboma-type pupil deformation, formation of prelental films, often pigmented. The condition is associated with active production of virus-neutralizing antibodies in titers of 1:4 to 1:16384. No relationship between the clinical course and antibody titers was revealed.

Antibody Formation↗