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Biomedical subjects

G A Padgett

Publications and source records attributed to G A Padgett.

At least 55 records · Page 3Linked to original sources
PubMed1972

Defective function of renal lysosomes in mice with the Chediak-Higashi syndrome.

Morphologically abnormal lysosomes demonstrated in individuals with the Chediak-Higashi syndrome (CHS) suggested a defect in the function of these abnormal lysosomes. To gain direct experimental evidence of such a defect, horseradish peroxidase (HRP) was injected intravenously into CHS and control mice, the mice killed at varying intervals and the kidneys studied by ultrastructural cytochemistry. No morophologic difference was observed in the absorption and uptake of HRP by proximal convoluted tubules in the two groups of mice. In CHS mice, however, some of the HRP fused with enlarged lysosomes. By 48 hours after injection, the lysosomes of normal mice had digested all but trace amounts of HRP, whereas large amounts were still present in CHS mice at this time. In CHS mice, moderate amounts were still present at 72 hours and trace amounts 96 hours post injection. This slowed rate of digestion of HRP by lysosomes of the proximal convoluted tubule cells of CHS mice suggests a similar defect in all cells in CHS individuals in which there is a lysosomal degradation of protein or other matter obtained by endocytosis. Such a defect may explain some manifestations of impaired host defense observed in CHS.

Animals↗
PubMed1969

Giant neutrophil granules in the Chediak-Higashi syndrome of man, mink, cattle and mice.

The percent of conventionally stained circulating neutrophils containing abnormal giant granules in Chediak-Higashi mink, cattle and mice is highly variable and strikingly less than the 100% found in neutrophils from similarly affected humans. In contrast, using either peroxidase or Sudan Black, it can be demonstrated that 100% of circulating neutrophils in all affected individuals of the four species studied contain the abnormal granules. Minor species differences in the shape of the granules were noted. The significance of these findings as well as the variations in granular staining by the several techniques employed are discussed.

Albinism↗