Biomedical subjects

G A Smirnova

Publications and source records attributed to G A Smirnova.

40 records · Page 3Linked to original sources

PubMedDate not supplied

[The development of nutrient media for the cultivation of recombinant Escherichia coli strains].

The study of the qualitative and quantitative composition of peptides and free amino acids in commercial casein hydrolysates, produced in Russia, has provided grounds for the choice of the nutrient base in the medium for the cultivation of E. coli recombinant strains. In the newly developed medium specific endoglucanase activity is 1.5- to 1.7-fold greater than in Luria-Bertani broth ("Difco" Laboratories, USA).

Bacterial Proteins↗

PubMedDate not supplied

[The development of bacterial growth stimulants from plants].

Dried bacterial growth stimulators in the form of water extracts of wild marjoram were developed; they produced a stimulating effect on Escherichia coli and Streptococcus pyogenes Dick 1 and had no influence on the growth of Corynebacterium xerosis 1911. The study aimed at finding out the active principle of the stimulator prepared from marjoram extract revealed that under experimental conditions marjoram extract could be divided into 3 fractions of these: fraction 1 contained terpenic hydrocarbons, fraction 2 contained vitamins and vitamin-like substances, fraction 3 contained phenols, flavonoids, phenolcarbonic and fatty acids. Fraction 3 at a concentration of 0.0001% produced the best effect. The stimulating effect of extracts obtained from lime and aspen leaves, haw berries was demonstrated.

Bacteria↗

PubMedDate not supplied

[Changes in the lipid component of the muscle tissue of several species of fish during refrigeration and heat treatment].

By using univariate thin-layer chromatography on silicagel IC254 of the company "Chamapod Széchoslovakia" the qualitative and quantitative composition of the muscular tissue lipids in new oceanic fish--seriolella and epigonus was determined. It was ascertained that the qualitative composition of lipids changed during cold storage but insignificantly, due to hydrolysis of triglycerides and oxidation of phospholipids. In the course of heat treatment there occurs disintegration of the lipid-proteinic complexes attended by an accretion of phospholipids. An increment of free fatty acids is caused by the hydrolytic decomposition of triglycerides.

Animals↗

PubMedDate not supplied

[Physiologic-biochemical features of Morganella under various conditions of cultivation].

The parameters of the cultivation process and the content of protein and polysaccharide in M.morganii cells grown under the conditions of submerged cultivation in 2 nutrient media having different composition were studied. The study revealed that the highest values of specific growth rate and the maximum yield of biomass were observed during cultivation under the conditions of medium values of oxygen concentration in the medium (pO2 = 20-30%). The content of protein in bacterial cells was practically independent on the growth phase of the culture, with the exception of cultivation processes in carbohydrate-free media with the use of "old" seed material (48-hours agar culture) when protein content in the cells was higher in the exponential phase. The content of polysaccharide in bacterial cells, its maximum level observed at the exponential phase, considerably dropped at the growth deceleration and stationary phases.

Bacterial Proteins↗