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Biomedical subjects

G Ansuini

Publications and source records attributed to G Ansuini.

16 recordsLinked to original sources
PubMed1989

Quantitative evaluation of recurrent meningiomas.

Clinical, histological and karyometric parameters, nuclear DNA content and the number of nucleolar organizer regions were investigated in 9 recurrent meningiomas and 10 meningiomas which had not recurred within a 10-year period. There were no significant differences between the two groups as to age, sex, site of the tumours and most of the histological parameters scored. Recurrent tumours showed a higher number of mitotic figures and the nucleolus was visible in most of the cells. Cell density, nuclear area, perimeter and nuclear DNA content had values with no statistically significant differences between the two groups. However, significant differences were found in the distribution of the nuclei in the different ploidy regions. Most of the nuclei in the non recurrent cases were in the diploid range, whereas in recurrent tumours there was a reduction in the number of diploid cells associated with an increase in 2c--4c and 4c components. Recurrent tumours also showed a higher number of nucleolar organizer regions positively stained using an argyrophil method. The mitotic count and the nucleolar organizer regions appeared to be the best predictors for recurrence.

Adult↗
PubMed1989

Clinical significance of the histomorphometric evaluation of diabetic microangiopathy in the oral mucosa.

Several structural parameters of the capillary vessels were measured in the oral mucosa of patients with diabetes mellitus of type 1 (D.1) and of type 2 (D.2), and of control cases (C), by means of an image analyser in histological sections of routinely processed biopsies. The studied parameters included: a) capillary wall thickness; b) capillary diameter; c) the ratio of capillary wall thickness and diameter; d) capillary wall area; e) capillary area; f) the ratio of capillary wall area and capillary area; g) density of capillary vessels in the lamina propria; h) density of endothelial cells; i) endothelial nuclear area. Clinical and laboratory parameters were also evaluated (duration of the disease, systolic and diastolic blood pressure levels, glycemia, glycosylated haemoglobin, glycosylated albumin, fructosamines, apolipoproteins A1 and B), in order to assess whether a relationship exists with the morphometric parameters studied. Statistically significant differences, at the level of p less than 0.05, were found in the following morphometric parameters between controls and each group of diabetic patients: mean and standard deviation of capillary wall thickness, mean capillary wall area, mean ratio of the capillary wall area and capillary area. A reduction in the capillary density, i.e. the number of capillary vessels per mm2 of lamina propria, was also observed in diabetic patients with respect to the control group, although it was not statistically significant (C vs. D.1: p less than 0.21; C vs. D.2: p less than 0.10).(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1988

Assessment of disease activity in essential cryoglobulinemia by serum levels of a basement membrane antigen, laminin.

When compared with 40 normal controls, 46 patients with essential cryoglobulinemia had higher serum concentrations of a basement membrane antigen, laminin fragment P1 (LP1). Serum LP1 values were more pronounced in patients with visceral involvement, compared with those with skin vasculitis only. Asymptomatic patients or those with arthralgia usually had normal values. Increased disease activity was associated with a significant increase in LP1 (P less than 0.01). For evaluation of disease activity, determination of serum LP1 was a more sensitive measure than any complement factor assay.

Adolescent↗
PubMed1988

Comparison of computerized analysis of nuclear DNA changes in uterine cervix dysplasia and in urothelial non-invasive papillary carcinoma.

The nuclear DNA content was measured in preneoplastic lesions of the uterine cervix and in papillary carcinomas of the bladder. Three groups of features were calculated from the raw data: basic DNA, DNA deviation and DNA distribution. The basic DNA features, concerning both the cervix and the bladder, showed a progressive increase in the mean DNA content, a decrease in the percentage of diploid nuclei and steadily increasing values of polyploid and aneuploid nuclei. Among the DNA deviation features, the malignancy grade value was zero in the normal cervical epithelium and in the normal urothelium. An increase in this value was evident in moderate dysplasia and in urothelial papillary carcinoma of grade 2, the highest value being in CIS and in grade 3. Concerning the DNA distribution features, the values of the 15th and 95th percentiles and their difference were progressively higher both in the cervix and in the bladder, expressing a continuous shift and spread of the DNA content measurements in the different diagnostic categories, with respect to normal epithelium and urothelium. The statistical analysis showed that the strongest correlation is between 2c D.I. and % of polyploid nuclei in the cervix and between M.I. and % of aneuploid nuclei greater than 4c in the bladder. In the cervix the most discriminating feature is the Malignancy Grade, whereas in the bladder it is the percentage of diploid nuclei. The comparison between the results of the three groups of features showed that: 1) Mild dysplasia of the cervix and urothelial papillary carcinoma of grade 1 showed similar changes in DNA features. Both were basically characterized by increased proliferative activity.(ABSTRACT TRUNCATED AT 250 WORDS)

Carcinoma, Papillary↗
PubMed1987

Prognostic value of computerized DNA analysis in noninvasive papillary carcinomas of the urinary bladder.

DNA parameters and a DNA status index were calculated in cases of noninvasive urothelial papillary carcinomas and apparently normal urothelium. DNA feature analysis included the measurement of basic parameters as well as those related to DNA deviation. Normal urothelium had a diploid content with a few values between 2c and 4c. The papillary lesions in the three grades showed a progressive decrease in diploid nuclei and steadily increasing percentages of tetraploid ones. One case of grade 1 papillary carcinoma and some of grade 2 showed a small proportion of aneuploid cells; the proportion quintupled in grade 3. The values of parameters related to DNA deviation increased progressively in the three grades, numerically expressing the shift from diploidy. The DNA status index is a multiparameter classification in which a single number, ranging from 0.5 to 3.7, expresses the degree of DNA deviation from a non-proliferating diploid population. The cases of normal urothelium and of grade 1 had the lowest values, from 0.5 to 1.9, whereas grade 3 cases had the highest, from 2.2 to 3.7. The index values of grade 2 cases were partly intermediate and partly in the range of the neighboring grades. The DNA status index has a prognostic significance. In fact, associated lesions and recurrences were only observed in cases with index values over 2.0.

Carcinoma, Papillary↗
PubMed1986

Multivariate classifications of transitional cell tumors of the bladder: nuclear abnormality index and pattern recognition analysis.

The authors report their experiences in applying two types of computer-aided multivariate classification systems in transitional cell tumors of the bladder. The systems are based on nuclear changes in urothelial papillary lesions. 19 out of 54 parameters, selected on the basis of the overlapping area between contiguous grades and the monotonic function, were combined mathematically to obtain the nuclear abnormality index. This index expresses the progressive nuclear abnormalities as a single number on a continuous scale from 0.5 to 2.6. The standard deviation (SD) of the 10 largest nuclear area values in the lower half of epithelial thickness (L), the mean perimeter (L), the SD of the roundness factor in the upper half of epithelial thickness (U), the SD of the logarithm of area (L) and the percentage of round nuclei (U) represent the smallest, the most discriminant and the least correlated set of features for the pattern recognition analysis. The latter shows an agreement of 92% between computer and histologic classifications-estimated by applying Bayes theorem. A good correlation exists between nuclear abnormality index and computer grading by pattern recognition analysis.

Carcinoma, Transitional Cell↗
PubMed1986

Benign and malignant mesothelial lesions of the pleura: quantitative study.

Benign and malignant mesothelial lesions of the pleura are quantitatively studied using a Leitz TAS image analyzing system. The Feulgen fast green staining method allows performing on the same cell measurements of the shape and size of the nucleus, the size of the nucleolus and the content of DNA. Numerical data are studied in a multivariate statistical analysis in order to obtain the probability of the morphometric diagnosis in each case. Results demonstrate that the most important measurements are the percentage of cells with evident nucleolus, the nuclear perimeter and the standard deviation of the DNA content, and that these measurements give a correct diagnosis in all cases.

Analysis of Variance↗
PubMed1986

Quantitative evaluation of the progressive nuclear abnormalities in urothelial papillary lesions.

The natural history of noninvasive urothelial papillary carcinomas is characterized by structural and nuclear abnormalities of the epithelial lining in the three grades. Morphometry is applied to measure nuclear-related quantitative parameters from the lower (L) and upper (U) halves of urothelial thickness, i.e. perimeter, area and roundness factor. Their mean and SD as well as the derived parameters have values steadily higher from normal-looking urothelium to G3, whereas the relative frequency distribution shows over-lapping among the grades. The multivariate analysis is then applied. First of all the smallest number of least correlated and most discriminant features is selected: SD of nuclear area log (L), percentage of round nuclei (L), mean of the 10 largest values of nuclear area (L) and mean nuclear perimeter (L). The results of the pattern recognition analysis based on these features show the following percentages of agreement between histopathological and computer classifications: 94% in the training set and 80% in the test set. The calculation of the classification probability and the adoption of a threshold approach of 0.25 less than P less than 0.75 are helpful in identifying the 'in-between' cases, i.e., those in the 'intermediate' position between G1 and G2 and between G2 and G3. Morphometry and multivariate analysis allow us to quantify the nuclear abnormalities of urothelial papillary carcinomas and show a progressive and continuous spectrum of modifications.

Cell Nucleus↗
PubMed1985

Noninvasive papillary transitional-cell tumors. Karyometric and DNA-content analysis.

An investigation was undertaken to quantify and correlate the nuclear and DNA-content modifications in the different grades of noninvasive urothelial papillary carcinoma. Nuclear area and a roundness factor were studied by histomorphometry in 35 cases, and the DNA content was analyzed in 27 cases. The values of the nuclear area and the roundness factor increased from normal-looking urothelium to grade 3 tumors. The DNA-content analysis indicated a progressive change of ploidy and the presence of an aneuploid (greater than 4c) population in some of the grade 2 and grade 3 lesions. A high coefficient of correlation between the nuclear abnormalities and the DNA content was found.

Aneuploidy↗
PubMed1984

Histomorphometry and pattern recognition analysis of urothelial papillary lesions.

The authors report the results of the grading of urothelial papillary carcinoma using histomorphometry and pattern recognition analysis. Features concerning nuclear area, the nuclear roundness factor and inclination angle were measured in 38 cases of urothelial papillary carcinomas. The relative frequency distribution showed overlapping among the grades with a reduction in the practical significance of histomorphometry. To overcome this problem, the pattern recognition analysis was applied. It was found that mean nuclear area, mean roundness factor and percentages of round and horizontal nuclei represent the optimal discriminating set of the subsequent steps in the malignant urothelial transformation.

Carcinoma, Papillary↗
PubMedDate not supplied

The value of morphometry in prediction of survival in oligodendrogliomas.

Nineteen out of 31 cases of well-differentiated oligodendrogliomas were selected on the basis of clinical and morphological parameters. The patients were divided into 3 groups according to the length of the survival period. In each case 8 clinical, 4 morphological and 3 morphometrical parameters were scored. The quantitative analysis of nuclei of oligodendroglioma cells, performed by means of an automated microscopic picture analyzer (Leitz-TAS), allowed the nuclear area, perimeter and roundness factor of 200 nuclei to be calculated for each case. Twenty-eight additional features were derived from the data obtained. The descriptive statistical analysis, based on Student's t-test and Chi-square test showed significant differences (p less than 0.05) with regard to the SD of the 10 largest values of the nuclear perimeter among the derived quantitative parameters. Among the clinical parameters, the increased intracranial pressure as a late complication was also significant. Multivariate analysis, based on Bayes theorem, allowed 89% of the cases to be allocated to the actual groups by means of 6 clinical parameters, 57% by means of 4 morphological parameters and 100% by means of a set of 3 morphometrical parameters. The morphometric data proved to be better discriminants than clinical and subjectively evaluated morphological parameters in low grade oligodendrogliomas.

Adolescent↗