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Biomedical subjects

G Arfellini

Publications and source records attributed to G Arfellini.

5 recordsLinked to original sources
PubMed1978

In vivo DNA repair after N-methyl-N-nitrosourea administration to rats of different ages.

DNA repair time-course was studied after injury by N-methyl-N-nitrosourea (MNU) in rat liver cells of animals of different ages and in fetuses using hydroxyurea (HU) as inhibitor of scheduled DNA synthesis. DNA repair was a rapid phenomenon, more so in young adults than in newborns, and was not detectable in fetuses. A correlation seems to exist among organ sensitivity to carcinogen, age of animal and DNA repair.

Age Factors↗
PubMed1977

Compared effects of N-hydroxyurethan, urethan and hydroxyurea on DNA synthesis. In vivo and in vitro studies.

The effect of N-hydroxyurethan (HUR) on DNA synthesis has been tested both in vivo on various tissues and in vitro on concanavalin A (ConA)-stimulated rat thymocytes and compared with the action of urethan and hydroxyurea. HUR suppresses scheduled DNA synthesis, except that of non-stimulated spleen cells in vitro. The inhibition is efficient and rapid and takes place immediately if the drug is administered at the peak of the S-phase. UR inhibits DNA synthesis in vitro only at much higher doses and with different time course. It is effective or slightly effective if it is administered at the peak of the S-phase. A conversion of urethan into HUR the latter depressing DNA synthesis could partly explain the differences observed. No toxicity was found after treatment with drugs at the concentration employed. Finally, the relationships between drug doses and cell responses have been particularly observed in vivo.

Animals↗
PubMedDate not supplied

DNA repair after UV and gamma irradiation. I. Rat spleen cells.

An in vitro microculture was set up to standardize DNA enzymatic repair in rat spleen lymphocytes after exposure to ultraviolet or gamma rays by measuring the tritiated thymidine uptake. A relation between irradiation dose and DNA repair is always observable when cell viability and scheduled DNA synthesis are taken into account. This micromethod utilizing rat spleen cells appears to be a suitable system for such a study since it permits a satisfactory evaluation of DNA repair.

Animals↗