Biomedical subjects

G Arivarignan

Publications and source records attributed to G Arivarignan.

4 recordsLinked to original sources

PubMed2006

Disease mapping using mixture distribution.

BACKGROUND & OBJECTIVES: Data on infectious diseases like tuberculosis (TB) have been analyzed in the past without giving adequate attention to spatial variations. Earlier studies also attempted to display disease status of sub regions, usually census tracts, by categorizing them into quartiles, that helps the authorities to identify high- or low-risk areas. This approach is based mainly on binomial and Poisson models for disease data, and the recent attempts focus on using mixture models of Poisson distribution. We carried out this study to find wards of Madurai Corporation having high risks for TB disease, to develop a model of mixture of Poisson distributions for the number of cases and to classify each ward to one of many risk groups for TB disease, and to represent spatial distribution of TB incidence in Madurai city. METHODS: produced the observed counts of TB patients in 72 wards of Madurai Corporation. The number of risk groups and the Poisson parameters of each group were found by maximum likelihood approach using the computer package C.A.MAN (Computer Assisted Mixture ANalysis). Bayesian methods were used to associate each ward to a particular risk group. The results were geographically presented in maps by using ArcView mapping software. RESULTS: Using binomial model, 26 wards were categorized as high risk wards, and with mixture model approach 15 wards showed standardized morbility ratio (SMR) >1. The wards along river Vaigai and densely populated wards had high risk. INTERPRETATION & CONCLUSION: Our findings demonstrate the usefulness of the mixture models for disease data with geographical variations.

Bayes Theorem↗

PubMed2003

Mycobacterium bovis BCG scar status and HLA class II alleles influence purified protein derivative-specific T-cell receptor V beta expression in pulmonary tuberculosis patients from southern India.

Purified protein derivative (PPD) RT23-recalled T-cell receptor (TCR) V beta expression was studied in the peripheral blood of 42 pulmonary tuberculosis patients and 44 healthy controls from southern India, a region where tuberculosis is endemic. Forty-eight-hour whole-blood cultures in the presence or absence of PPD-RT23 were set up, and at the end of the culture period total RNA was extracted and cDNA was synthesized. Expression of various TCR V beta families was assessed by using family-specific primers. PPD-specific expression (usage) of TCR V beta families 4, 6, 8 to 12, and 14 was found in more controls than patients. Among the responders (individuals who showed PPD-specific expression), endemic controls had significantly higher responses than the patients had for TCR V beta families 2, 3, 7, 13, and 17. The majority of the patients did not show usage of most of the TCR V beta families, and this was attributed to T-cell downregulation. A four-way nested classification analysis revealed that TCR V beta family 1, 5, 9, 12, and 13 usage in the context of HLA class II high-risk alleles (DRB1*1501, DRB1*08, and DQB1*0601) and Mycobacterium bovis BCG scar status were the determining factors in susceptibility and resistance to tuberculosis. The healthier status of controls was attributed to the wider usage of many TCR V beta families readily recalled by PPD, while the disease status of the patients was attributed to TCR V beta downregulation and the resultant T-cell (memory cell?) unresponsiveness. Host genetics (HLA status) and BCG vaccination (scar status) seem to play important roles in skewing the immune response in adult susceptibility to pulmonary tuberculosis through TCR V beta usage.

Adult↗

PubMed2002

Roosting patterns in a captive colony of short-nosed fruit bat Cynopterus sphinx (Vahl).

Development of roosting patterns under a limited resource was studied in the short-nosed fruit bat C. sphinx in captivity. Spatial fidelity during the resting period (day time) and the individual male bat's presence/absence in the roost (occupancy index) were estimated during the active period (night time). Results show the presence of three groups on the basis of spatial fidelity. The first group was associated with the tent consisting of a harem male and seven females. The second group stayed near to the harem. The third group consisting of two males showed little occupancy index and no spatial fidelity. Female turnover between the first and second groups, and harem male replacement were observed. These findings of male groupings and female loyalty on the basis of "resource", suggest that resource defence polygyny is the primary mating strategy in C. sphinx.

Animals↗

PubMed2001

Association of interleukin-10 cytokine expression status with HLA non-DRB1*02 and Mycobacterium bovis BCG scar-negative status in south Indian pulmonary tuberculosis patients.

HLA DRB1*02 and its subtypes predispose individuals for a far-advanced sputum-positive pulmonary tuberculosis transcending ethnic boundaries. Mycobacterium bovis BCG does not afford the desired protection against adult pulmonary tuberculosis, and a spectrum of immune reactivity exists in controls and hospital contacts. All of these findings have been identified and demonstrated in areas of endemicity. Skewing of immunity from protective to pathogenic may involve a shift in the Th1-Th2 paradigm. To elaborate these ideas, we studied gamma interferon (IFN-gamma), interleukin-4 (IL-4), and IL-10 cytokine expression in 71 adult pulmonary tuberculosis patients and 74 controls from areas of endemicity in south India by 48-h microculture and reverse transcription-PCR. Most of the patients and controls expressed IFN-gamma de novo, and in the presence of purified protein derivative (PPD), all of them expressed significantly higher levels of IFN-gamma, suggesting a PPD-specific recall memory. HLA DRB1* allele-dependent IFN-gamma expression was identified only in controls, suggesting a skewing of the immune response in patients. In contrast to the case for IFN-gamma, only some patients and controls expressed IL-4 or IL-10 (Th2 profile); thus, the Th1 profile was identifiable only by a nonexpression of IL-4 or IL-10 in this area of endemicity. The Th2 profile was associated with HLA non-DRB1*02 and BCG scar-negative status in patients, attributing a significant risk (odds ratio = 2.074; 95% confidence interval = 0.612 to 7.07). It is possible that Mycobacterium tuberculosis (PPD)-specific IL-10 is expressed preemptively in unvaccinated (BCG scar-negative) individuals with a non-DR2 genetic background by chronic exposure in this area of endemicity and leads to pulmonary tuberculosis of adults.

Adult↗