[Encephalitis simulating thyrotoxicosis].
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Biomedical subjects
Publications and source records attributed to G Avram.
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On lines of human (HeLa) and monkey (BS-C-1) cell cultures, the cytotoxicity of 15 products of dental use for radicular and coronary fillings, alloys and endodontic antiseptics was analysed. It was found that the simplified method can be used, together with the in vivo and in vitro tests recommended by I.S.O., for determining the bioavailability of dental products.
657 hospitalized children with acute diarrheal disease were studied for bacterial and rotaviral etiology. Cryptosporidium presence was followed in 123 children. Intestinal pathogens were detected in 195 (29.6%) cases: 132 (20.3%) enterobacteria, 47 (7.1%) rotaviruses, 4 (3.2%) Cryptosporidium sp. and 12 (1.8%) combined infections. Among enterobacteria, E. coli was the most frequent (10% cases) with enteropathogenic (EPEC), enterotoxigenic (ETEC), enterohemorrhagic (EHEC) and enteroinvasive (EIEC) groups. Other isolated pathogens were Salmonella--21 (3.1%), C. jejuni/coli--13 (1.9%), Shigella--3 (0.9%), Y. enterocolitica O3--1 (0.1%). Among opportunistic pathogenic enterobacteria, the following were isolated: Kl. pneumoniae--24 (3.7%), Enterobacter species--4 (0.6%), Providencia alcalifaciens--1 (0.1%). Aeromonas hydrophila was isolated in 1 child. Combined infections were detected in 12 children: 10--enterobacteria + rotaviruses associations and 2--pathogenic enterobacteria associations.
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Enteroviruses were isolated from 12% of the digestive tract samples collected from Anodonta cygnea (9.4%) and Dreysena polymorpha (13.2%) shell species and from 8.4% of the branchiae sample from fish species Rutilus rutilus, Perca fluviatilis and Silurus glanis. The isolated belonged to the following antigenic types: ECHO 2 and 7, Coxsackie B 2, 4 and 6, and poliovirus 1 and 2.
Enteroviruses were isolated from 4.6% out of the examined tap water samples. No relation could be established between viral pollution and classic quality indexes of water.
The rotaviral antigen was detected by a screening test using the ELISA-IC kit in 17.6% out of 415 children with acute gastroenteritis. The highest frequency (28.9%) was found in children hospitalized in pediatric services with a diagnosis of diarrhoeic disease associated to acute respiratory infection. The rotavirus infection incidence was about three times higher during the cold season than during summer (30.4% versus 10.5%). The 6-11 month age group was the most severely affected.
Fecal specimens from 84 children with gastroenteritis were submitted to virological and bacteriological analysis. Enteroviruses other than poliovirus were found in 8.3%, rotavirus in 4.8% and enterobacteria in 1.9% of the investigated subjects. Mixed infections were detected in 8.4% of the cases.
Studies conducted on 417 feces samples collected from newborn infants from seven maternity homes revealed the presence of rotavirus in 1.2 to 9.5% of the subjects. The infants get infection during the first 24 to 48 hours of life (1.8%) and the positivity rate reaches a peak the 7th day (9.7%). Enteroviruses were found in 3.4% and enterobacteria in 11.8% of the samples.
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In three children communities covered by a virological study enteroviruses could be isolated from 19.3% of the stool samples, 52.3% of the sewage samples and 9.7% of the samples taken from different objects and surfaces. The isolates belonged to 14 enterovirus serotypes, Coxsackie viruses being predominant. There was a good concordance between the serotypes of enteroviruses shed in the stools and those isolated from the environment. The high epidemiological potential of enteroviruses calls for a surveillance of their circulation in children communities, preferably by the virological monitoring of sewage.
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Experimental studies using dyes and microbial agents as indicators showed that a viral contamination of the various surfaces of the water closets is possible through the droplets formed when one flushes the W.C. pan. An investigation conducted in several public water closets allowed to reveal the presence of enteroviruses in 2.8% to 5.8% of the samples collected from various surfaces of the water closets. The results of the investigations showed that the hinged seats were contaminated the most frequently (6.8% of the samples).
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