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Biomedical subjects

G B Irvine

Publications and source records attributed to G B Irvine.

At least 55 records · Page 3Linked to original sources
PubMed1990

Development of a radioimmunoassay for neurone specific enolase (NSE) and its application in the study of patients receiving intra hepatic arterial streptozotocin and floxuridine.

A radioimmunoassay has been developed for neurone specific enolase (NSE) and used to measure serum NSE levels in patients with neuroendocrine and non-neuroendocrine tumours following intra hepatic arterial chemotherapy. Ten patients were studied, 7 receiving streptozotocin and floxuridine for neuroendocrine tumours and three receiving cisplatinum for non-neuroendocrine neoplasms. All ten patients had liver metastases. In patients with tumours of neuroendocrine origin, a significant increase in serum NSE was recorded within 24 h of therapy. Slight increases in serum NSE levels were also recorded in three patients with non neuroendocrine tumours. These increases may reflect lysis of neuroendocrine cells within the tumour. Raised levels in non-neuroendocrine tumour patients may reveal damage done to healthy neuronal and neuroendocrine cells during treatment. NSE may be a useful marker of the extent of cell death following chemotherapy.

Animals↗
PubMed1990

Visual detection of peptidase activity using fluorogenic substrates in a microtiter plate assay.

A simple, inexpensive, and sensitive assay for peptidase activity has been devised. The assay was performed in a microtiter plate and was based on fluorogenic peptide substrates, many of which are commercially available. 7-Amino-4-methyl coumarin the fluorescent product liberated during an incubation period of between 1 and 16 h, was detected by inspection of the plate under ultraviolet light of wavelength 356 nm. A fluorometer was not required. Using alpha-chymotrypsin as a model enzyme, with succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-methyl-coumaryl-7-amide as substrate, it was shown that as little as 4 fmol of enzyme could be detected. The method was non-quantitative and was particularly suited to location of enzyme activity in fractions during a purification procedure. The validity of the assay was demonstrated by detection of activity of a known enzyme, alpha-chymotrypsin, after its purification by size-exclusion high-performance liquid chromatography. The method was used to locate two forms of aminopeptidase activity, in fractions from size-exclusion chromatography of an extract from reproductive tissue of Helix aspersa, using L-leucine 4-methyl-coumaryl-7-amide as substrate.

Aminopeptidases↗
PubMed1989

Determination of bile acids and their conjugates in serum by HPLC using immobilized 3 alpha-hydroxysteroid dehydrogenase for detection.

A method is described for the separation of unconjugated bile acids and their glycine and taurine conjugates in a single step with adequate sensitivity for analysis of serum samples. Separation was carried out over a period of 80 min using a linear gradient with increasing concentrations of methanol in aqueous ammonium dihydrogen phosphate buffer, on an ODS Spherisorb column. Spectrofluorometric detection of NADH, formed as the column eluate passed through a column of immobilized 3 alpha-hydroxysteroid dehydrogenase, enabled amounts less than 40 pmol to be quantified. The reaction was carried out at neutral pH so that the lifetime of the enzyme column was increased, compared to other methods where a pH nearer 9.0 is commonly used. Flow rates were optimized to give comparable peak area for both primary and secondary bile acids.

3-Hydroxysteroid Dehydrogenases↗
PubMed1988

Articular fractures of the distal scaphoid.

We propose an anatomical classification of fractures involving the distal articular surface of the scaphoid. The predominant patterns, in the 37 patients reviewed, were avulsion fractures from the radio-volar tip of the tuberosity and impaction fractures of the radial half of the articular surface. The possible mechanisms of injury are discussed.

Adolescent↗
PubMed1988

Fractures of the femoral neck in elderly patients with hyperparathyroidism.

Five fractures of the femoral neck were observed in patients with primary or secondary hyperparathyroidism. In each case, the fracture line was vertically oriented near the base of the neck. The diagnosis was suggested by the patient's history and roentgenologic features and was confirmed by biochemical changes. None of the fractures healed despite internal fixation in four cases and appropriate treatment of the underlying disease. When such fractures occur in elderly patients, primary arthroplasty may be preferable to internal fixation.

Aged↗
PubMed1987

High-performance size-exclusion chromatography of polypeptides on a TSK G2000SW column in acidic mobile phases.

Polypeptides subjected to high-performance liquid chromatography on a TSK G2000SW column in acidic mobile phases of low ionic strength underwent a combination of size exclusion and ion exclusion from the matrix. The distribution coefficient (Kd) of each polypeptide was dependent upon the concentration of ion-pairing agent present and increased as the concentration of ion-pairing agent increased. The fractionation range of the column could thus be manipulated by altering the concentration of acid in the mobile phase. The nature of the ion-pairing acid, in terms of hydrophilic or hydrophobic ion pairing, also had an effect on the chromatography, such that hydrophobic ion-pairing agents lead to increased Kd values, especially in the case of the most basic polypeptides. To account for these results it is proposed that the TSK GSW matrix is cationic at low pH, and that ion pairing suppresses the ion exclusion experienced by cationic polypeptides under these conditions.

Chromatography, Gel↗
PubMed1987

The long-term results of localised anterior cervical decompression and fusion in spondylotic myelopathy.

A retrospective analysis is presented of 46 patients with cervical spondylotic myelopathy treated by anterior cervical decompression and fusion. In each case the spondylosis was localised and a maximum of two intervertebral levels was fused. Operation had been carried out 7.0 to 14.4 years (mean 10.0 years) previously and the mean age at surgery was 60 years. Disability was such that no patient was able to work prior to operation and only 10 patients (22%) could walk unaided. The functional results, in terms of walking ability, show that 36 patients (78%) have been improved, six (13%) remain unchanged and in 4 patients (9%) the disease has progressed. Twenty nine patients (63%) were able to walk without aid at the time of review. On a subjective basis 30 patients (65%) felt that they had benefitted from surgery.

Adult↗