Use of internal standards in electrophoretic surveys of enzyme polymorphism.
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Biomedical subjects
Publications and source records attributed to G B Johnson.
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Estimation of the effective number of alleles in natural populations from the frequencies of electrophoretically detected alleles involves significant ambiguity, which should be explicitly considered in the estimate. For large genetic populations the ambiguity importantly affects the value of n(e).
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The use of internal standards in the separation of proteins by gel electrophoresis is shown to offer significant advantages over other control procedures heretofore used in electrophoretic surveys of enzyme variation in natural populations. By the use of such internal standards, a previously undetected class of micro-variability among the enzymes of wild insects has been demonstrated.
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