Biomedical subjects

G Bahrami

Publications and source records attributed to G Bahrami.

3 recordsLinked to original sources

PubMed2005

Fatty acid composition of human milk in Western Iran.

OBJECTIVE: To investigate the fatty acid composition of mature human milk in Western Iran with special focus on trans fatty acids. DESIGN: Observational study. METHODS: Milk samples were collected from 52 lactating mothers aging 19-39 y, from Western Iran. Subjects were asked to complete a diet questionnaire. Milk fatty acids were measured as 2-nitrophenylhydrazide derivatives by high-performance liquid chromatography. RESULTS: Saturated fatty acids were the main fraction of human milk (41.3%). Medium-chain fatty acids (C8:0-C14:0) constituted 24%, oleic acid (C18:1omega9) accounted for 30.9% and elaidic acid (C18:1T), the trans isomer of oleic acid, comprised 11.3% of the total milk fatty acids. Linoleic (C18:2omega6) and linolenic (C18:3omega3) acid contents were 13.8 and 1.1%, respectively. The level of the polyunsaturated fatty acids was 1.4% for arachidonic (C20:4omega6) and 0.2% for eicosapentaenoic (C20:5omega3) acid. CONCLUSIONS: The milk from Iranian lactating mothers, as compared to that from the American or European mothers, contained high levels of medium-chain and trans fatty acids. This difference may be attributed to the maternal diet with low animal protein and animal fat but with high carbohydrate and partially hydrogenated vegetable oils that carry large amounts of trans fatty acids. As the detrimental effects of trans fatty acids on blood lipids and cardiovascular diseases have been emphasized in the literature, a reduction of trans fatty acid content in the diet of Iranian mothers is suggested. SPONSORSHIP: Kermanshah University of Medical Sciences.

Adult↗

PubMed2003

Bitewing examination with four digital receptors.

OBJECTIVES: To evaluate recording errors and patient discomfort during bitewing examinations using four digital receptors. METHODS: Seventy-eight patients had two bitewings taken on each side of the mouth with the intention of displaying the tooth surfaces from the distal surface of the canine to the distal surface of the most posterior molar, using four digital receptors, two charge-coupled device (CCD) and two photostimulable phosphor (PSP) systems. The patients scored their feelings of discomfort during the examination on a visual analogue scale. Receptor positioning errors in the sagittal plane were determined from the tooth surfaces present on each image and in the vertical plane from the presence of the alveolar bone crest. Cone positioning errors were determined from cone cuts. RESULTS: Canine and premolar surfaces were more often not depicted on the CCD images than on the PSP images (P<0.05). Cone cuts occurred in 19% of DenOptix images, in 9% of Digora images and in one Planmeca image. The bone crest was more often missing in the upper jaw on Planmeca images than on PSP images (P<0.01). In the lower jaw, Trophy images more often missed the bone crest than the other systems (P<0.05). Patients ranked the receptors as follows (with increasing discomfort): DenOptix, Planmeca, Digora and Trophy, with all being significantly different (P<0.05). CONCLUSIONS: It was more difficult to correctly position CCD sensors than PSP plates in the vertical plane, resulting in more images with missing alveolar bone crest. CCD sensors most often did not display the most anterior surfaces in a bitewing examination.

Adolescent↗

PubMed2001

A rapid reversed phase high performance liquid chromatographic method for determination of etoposide (VP-16) in human plasma.

A rapid, simple and sensitive isocratic High Performance Liquid Chromatography (HPLC) method was developed to measure the concentration of etoposide in plasma samples with UV detection at 220 nm. The method uses a Bondapac C18 column at 60 degrees C. The mobile phase consists of Methanol: water (45:55 v/v) at a flow rate of 2.8 ml/min. Phenacetin was used as an internal standard. The plasma samples were extracted using ether with the organic layer evaporated under nitrogen. The residue was dissolved in 200 microl methanol with 20 microl injected into the HPLC column. The extraction method showed a recovery of 91.5+/-3% for etoposide. In this system, the retention time of phenacetin and etoposide were 3.3 and 4.4 min, respectively. The limit of detection of etoposide in plasma is 20 ng/ml and the limit of quantitation is 40 ng/ml. This analytical method has very good reproducibility (8.1% between-day variability at a concentration of 50 ng/ml). It is a fast, sensitive and economic method applicable for clinical and pharmacokinetic studies.

Chromatography, High Pressure Liquid↗