Some aspects of the effect of cyproterone acetate on levels of other steroid hormones in man.
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Adult↗
Biomedical subjects
G Barnard
Publications and source records attributed to G Barnard.
Estimation of number, mean size and size distribution of human spermatozoa in oligospermia using a Coulter counter.
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Acetates↗
The prognostic value of EEG sleep recordings in carbon monoxide intoxication.
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Adult↗
Neutral lipids of plasma and adipose tissue in a-betalipo-proteinaemia.
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Adipose Tissue↗
The measurement of prion protein in bovine brain tissue using differential extraction and DELFIA as a diagnostic test for BSE.
A simple diagnostic test for the detection of bovine spongiform encephalopathy (BSE), based on a commercially available time-resolved fluorescence immunoassay (DELFIA) for the measurement of the normal and disease-associated isoforms of prion protein (PrP), is described. The isoforms are sequentially extracted from homogenized bovine brain tissue using two concentrations of guanidine hydrochloride. This procedure initially extracts a soluble isoform and subsequently a less soluble disease-associated aggregated isoform. Following quantification of the two fractions, the percentage of the insoluble prion becomes a measurable parameter, independent of protein concentration, clearly identifying normal from infected animals displaying clinical signs of BSE. The mean percentages of insoluble PrP in brain tissue from 60 BSE-confirmed-positive cattle and 100 cattle that had never been exposed to the disease were 52.6% (SD = 22.8) and 3.9% (SD = 1.5), respectively. The assay is sensitive, with a detection limit of less than 50 pg PrP, and is robust and precise (CVs < 10%) over the appropriate working range.
Animals↗