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Biomedical subjects

G Biberfeld

Publications and source records attributed to G Biberfeld.

At least 217 records · Page 12Linked to original sources
PubMed1980

Demonstration of antibodies to double-stranded DNA by the Crithidia luciliae immunofluorescence test.

All of 627 sera positive for antinuclear antibodies (ANA) among 1,862 sera received during a three-month-period for routine examination of ANA were tested for antibodies to native double stranded (ds) DNA by the Crithidia luciliae kinetoplast immunofluorescence test. Eighty-seven (14%) of the ANA-positive sera had antibodies to dsDNA. These sera were derived from 73 patients. Forty out of 73 patients with dsDNA antibodies in their sera had the clinical diagnosis SLE. Among the other 33 patients there were eight cases diagnosed as rheumatoid arthritis, one case of psoriatic arthritis, four cases of unclassified arthritis, one case of polymyalgia rheumatica, five cases of chronic hepatitis, three cases of chronic glomerulonephritis and 11 cases with various other disorders. Antibodies to dsDNA, even at high titre, were demonstrated both in patients with clinically active and in patients with clinically inactive SLE.

Animals↗
PubMed1980

PPD-induced viral antibody production in human blood lymphocytes.

The ability of purified protein derivative of tuberculin (PPD) to induce polyclonal antibody production in cultures of human blood lymphocytes was studied. IgG and IgM were determined by the enzyme-linked immunosorbent assay (Elisa). PPD induced both IgM and IgG production, with a predominance of IgM. PPD usually stimulated a stronger IgM response but a weaker IgG response than did pokeweed mitogen (PWM). Supernatants of PPD- or PWM-stimulated lymphocyte cultures were tested for antibodies to morbilli, rubella and herpes simplex by Elisa. PPD as well as PWM induced viral antibody production in lymphocytes of donors who had serum antibodies to the corresponding viral antigens. Production of viral antibodies of IgG class but not of IgM class was demonstrated. The PPD-induced antibody response was T cell-dependent.

Adult↗
PubMed1979

T lymphocyte subpopulations in synovial fluid of patients with rheumatic disease.

The proportion of T lymphocytes with receptors for the Fc portion of IgG (TG cells) of IgM (TM cells) was determined in synovial fluid and blood of 16 patients with various rheumatic diseases including rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis. The percentage of TG cells was low in all synovial fluid samples, whereas in the patients' blood the percentage was higher than or equal to the level found in the blood of healthy subjects. Eight patients also had a lower level of TM cells in synovial fluid as compared to the percentage found in the blood of healthy donors. In the patients' blood the percentage of TM cells was usually within the normal range. Thus the proportion of T cells lacking either receptor (Tnull cells) was higher in synovial fluid than in blood. This pattern of low TG cell and high Tnull cell percentages was found in the synovial fluid of patients with various rheumatic diseases and thus seems to be a general feature of chronic inflammatory joint exudates.

Arthritis, Rheumatoid↗
PubMed1979

The effect of irradiation on T-cell suppression of ELISA-determined Ig production by human blood B-cells in vitro.

Human blood B-lymphocytes were co-cultured with in vitro irradiated allogeneic or autologous T-lymphocytes in the presence of pokeweed mitogen (PWM). The production of IgG, IgM and IgA, as assessed by the enzyme-linked immunosorbent assay (ELISA) was increased 2--7 times, as compared to values obtained with non-irradiated T-lymphocytes. It is suggested that the increase of Ig production is due to the selective radiosensitivity of T-lymphocytes with suppressor function.

B-Lymphocytes↗
PubMed1979

Autoimmune reactions associated with Mycoplasma pneumoniae infection.

Patients with Mycoplasma pneumoniae infection often develop various autoantibodies including cold agglutinins, cold antibodies reacting with lymphocytes, antibodies against various tissue antigens, for instance brain and lung and smooth muscle antibodies. Various mechanisms that may be responsible for the induction of these autoimmune responses and the possible pathogenicity of these autoantibodies are discussed.

Agglutinins↗
PubMed1979

The effect of in vitro irradiation on PHA-mediated cytotoxicity and lymphocytes with receptors for the Fc part of Ig.

In vitro irradiation of purified human lymphocytes reduced PHA-mediated cytotoxicity (MICC) against chicken erythrocytes in a dose-dependent fashion. Lymphocytes with receptors for IgG present in both unfractionated and T-cell enriched cell preparations were depleted after X-ray exposure. T-cells with IgM-receptors, however, appeared to be more resistant. Different lymphocyte subpopulations seem to cooperate in MICC, and shifts of their proportions may be one explanation of the reduction of MICC after X-ray exposure.

Animals↗
PubMed1977

Activation of human lymphocyte subpopulations by Mycoplasma pneumoniae.

B- and T-cell-enriched preparations of lymphocytes from human blood (5 cases), adenoids (10 cases), and tonsils (4 cases) were examined for the in vitro stimulation response to Mycoplasma pneumoniae (MP). B lymphocytes did not respond to MP by DNA synthesis except for the B cells from one adenoid. T lymphocytes and nonfractionated lymphocytes from blood, from three of the adenoids and two of the tonsils showed a stimulation response to MP. Nonfractionated adenoid lymphocytes from altogether 22 children were examined, and a stimulation response to MP was demonstrated in 8 cases. Lymphocyte cultures from 11 adenoids and 2 tonsils were examined for MP-induced non-antigen-specific antibody production by a hemolytic plaque assay. In all these cultures MP activated production of antibody-secreting cells to sheep erythrocytes. It is concluded that MP can induce non-antigen-specific angibody production in human B lymphocytes without stimulating DNA synthesis in these cells. The proliferative response of adenoid, tonsil, and blood lymphocytes to MP is interpreted as an antigen-specific T-cell response.

Adolescent↗
PubMed1977

Chronic active hepatitis in children. A clinical and immunological long-term study.

Six girls and one boy with cronic active hepatitis (CAH) of unknown etiology were between 9 and 15 years at the clinical onset of their illness. After beginning immunosuppressive therapy the course of their disease was followed from one to ten years. All had markedly increased IgG, high titres of smooth muscle antibodies (SMA) and antinuclear antibodies of IgG class in the earliest serum specimens tested. Therapy resulted in an improved sense of well-being and a decrease in SGOT, IgG and titres of SMA. Very high titres of measles antibodies were observed in all cases. In one of the cases CAH manifested itself after measles and in another after rubella infection. The first case in our series of patients died of liver failure after 5 years of illness. The other patients have survived and are able to live a normal life. The possibility of CAH should be considered when children develop symptoms of hepatitis. Longterm immunosuppressive treatment with regular clinical and laboratory evaluation is important. Estimation of titres of SMA is an additional parameter of value in following of the activity of CAH in these young patients.

Adolescent↗
PubMed1977

The reaction of cells with anti-actin sera in relation to the amount of cellular actin.

The staining pattern of anti-actin sera on various cells smeared on glass was compared to the relative amount of cellular actin estimated by SDS-polyacrylamide-gel electrophoresis with subsequent scanning of the gel. Although the cells showed a varying stainability the actin content was fairly constant. Thus, the staining differences reflected changes in the organization of cellular actin rather than actual differences in the amount of actin.

Actins↗
PubMed1976

Effect of Mycoplasma pheumoniae infection on cell-mediated immunity.

The effect of Mycoplasma pneumoniae infection on cell-mediated immunity was examined by two methods: the first method was the tuberculin skin test and the other was the measurement of the in vitro lymphocyte stimulation response to purified protein derivative (PPD) of tuberculin. Twenty-nine patients out of a total of 47 patients with a lower respiratory tract illness caused by M. pneumoniae had a negative tuberculin skin test when first tested. Twenty-three out of 26 patients with a negative tuberculin reaction in the early phase of the illness had a positive skin test when they were retested several weeks or months after the illness. The lymphocyte stimulation response to PPD was examined in 13 patients. In eight of these cases the lymphocyte response to PPD was significantly lower during illness than after recovery. Six control subjects showed no significant difference in their lymphocyte responsiveness to PPD when examined on two different occasions. These studies show that M.pneumoniae infection causes transient depression of cell-mediated immunity.

Humans↗
PubMed1976

Antibodies of surface antigens of lymphocytes and lymphoblastoid cells in cold-agglutinin-positive sera from patients with Mycoplasma pneumoniae infection.

IgM antibodies reacting in the cold with surface antigens of normal human blood lymphocytes were demonstrated by indirect immunofluorescence (IFL) in cold-agglutinin-positive sera from 21 patients with Mycoplasma pneumoniae (MP) infection. Twenty to fifty per cent of the lymphocytes were stained. MP sera reacted also with 75%-100% of cells from two lymphoblastoid cell lines and one Burkitt lymphoma line and with about 80% of peripheral blood lymphocytes from one patient with chronic lymphatic leukaemia. The IFL reaction was negative with cells from leukaemic lymphoid T-cell line (Molt-4). Lymphocyte fractionation experiments gave no evidence of a selective reactivity of MP sera and B or T lymphocytes of peripheral blood. Removal of cold agglutinins from the MP sera by absorption with human O erythrocytes significantly reduced the IFL reactivity with lymphocytes and lymphoblastoid cells, indicating the presence of I antigen on these cells. Furthermore, lymphoblastoid cells but not Molt lymphoid cells absorbed out most of the cold erythroagglutinins.

Agglutinins↗
PubMed1976

Tuberculin anergy in patients with Mycoplasma pneumoniae infection.

Skin testing for delayed hypersensitivity to tuberculin was performed in 36 patients with pneumonia associated with Mycoplasma pneumoniae infection. 22 of these patients had a negative tuberculin reaction when first tested. The tuberculin reaction was negative in 13 out of 16 cases tested during the first 2 weeks after onset of illness, in 6 out of 11 cases tested 15-28 days after onset of illness and in 3 out of 9 cases tested 29-45 days after clinical onset. In 19 patients with a negative tuberculin test in the early phase after onset of illness the test was repeated several weeks or months later. 16 of these cases had a positive tuberculin reaction at the later time period. Two of these cases showed prolonged tuberculin anergy for more than 5 months.

Adolescent↗
PubMed1976

Smooth muscle antibodies in Mycoplasma pneumoniae infection.

Paired sera from forty-five cases of Mycoplasma pneumoniae (MP) infection associated with acute lower respiratory tract illness were examined by immunofluorescence for antibodies to smooth muscle. Twenty-five (56%) of these cases had smooth muscle antibodies (SMA) of IgM class. A significant (greater than or equal to 4-fold) increase in titre of these antibodies was demonstrated in fifteen of thirty-five patients with a significant rise in titre of MP antibodies. SMA of IgG class occurred in eleven of forty-five cases (24%), but a 4-fold rise in antibody titre was found only in two cases. Three of forty-five sera (7%) from healthy donors contained SMA of IgM class and eight sera (18%) SMA of IgG class. MP antigen did not absorb SMA. Liver tests were performed in twenty-nine patients. In eighteen patients SGPT values were moderately or slightly elevated. There was no correlation between the occurrence of increased levels of transaminases and the presence of SMA in serum. In a patient with active chronic hepatitis, who had had a high titre of SMA exclusively of IgG class for 2 years, SMA of IgM class appeared transiently in association with an acute respiratory illness due to MP.

Antibodies↗
PubMed1976

Anti-actin specificity of human smooth muscle antibodies in chronic active hepatitis.

Thirty sera reacting by IFL technique in titres greater than or equal to 100 with smooth muscle fibres of rat stomach, rat renal glomeruli, and with the membrane region of thyroid cells were randomly chosen among sera sent in for routine testing of tissue antibodies. All sera but one were found to be derived from patients with chronic active hepatitis. The smooth muscle and other relevant cell staining were abolished after absorption of sera with actin, prepared from rabbit skeletal muscle and found to be homogeneous by SDS gel-electrophoresis and by electron microscopy. The actin anti-bodies were purified by precipitation of sera with F-actin and elution of the precipitates at acid pH. The purified antibodies stained all tissues in the same way as the original sera. In double immunodiffusion tests all thirty sera gave precipitation with actin. Thus, it was concluded that these broad-reacting SMA are directed against actin. The finding of high-titred SMA is of diagnostic value and supports the clinical diagnosis of active chronic hepatitis. In addition, anti-actin antibodies eluted from human sera are a suitable tool for studying actin-containing cellular structures.

Actins↗
PubMed1975

A plaque technique for assay and characterization of antibody-dependent cytotoxic effector (K) cells.

Using monolayers of erythrocytes as target cells, a plaque assay was developed by which individual antibody-dependent cytolytic lymphoid cells (K cells) could be identified at the cellular level. The extent of plaque formation depended on the concentration of sensitizing antibody, time of incubation, and number of lymphocytes added. Most of the plaque-forming cells (50% to 70%) were shown to possess receptors for activated complements, a small but variable fraction (5% to 25%) had surface Ig detectable by indirect immunofluorescence, and 5% to 10% bound sheep erythrocytes at 5 degrees C. The plaque-forming cells appeared as villous, small to medium-sized lymphocytes when studied by transmission or scanning electron microscopy. Under standardized experimental conditions the minimal number of active, plaque-forming K cells in purified lymphocyte preparations could be estimated.

Antibody Formation↗