Biomedical subjects

G Biberfeld

Publications and source records attributed to G Biberfeld.

257 records · Page 15Linked to original sources

PubMedDate not supplied

A formalin inactivated whole SIVmac vaccine in Ribi adjuvant protects against homologous and heterologous SIV challenge.

Eight monkeys were immunized at 0, 4, 9, and 18 weeks with a total of 2 mg of formalin inactivated SIVmac vaccine with Ribi adjuvant. Two weeks after the last booster four immunized monkeys and two controls were challenged with 10 MID50 of live homologous virus SIVmac, and the remaining four vaccinated animals along with two controls were challenged with the heterologous SIVsm strain. All eight vaccinated monkeys resisted the virus challenge, whereas all controls became infected. Three months after the first challenge the monkeys were rechallenged with the same virus strain, without further boosting. Two of four vaccinated monkeys were still resistant to the homologous SIV strain, and three of four monkeys were resistant to the heterologous SIVsm strain. This study demonstrates vaccine induced cross-protection between SIV strains.

Adjuvants, Immunologic↗

PubMedDate not supplied

Differences in the distribution of synovial T lymphocyte subpopulations between patients with acute and chronic exudative synovitis.

T lymphocyte subpopulations were determined by monoclonal antibodies Leu 2 (suppressor/cytotoxic subset) and Leu 3 (helper/inducer subset) in synovial fluid (SF) and blood of 9 patients with acute non-septic synovitis (duration less than 1 month), 28 patients with chronic synovitis (16 with rheumatoid arthritis (RA), 7 with ankylosing spondylitis, 4 with psoriatic arthritis and one with juvenile rheumatoid arthritis) and 37 age- and sex-matched healthy controls. The patients with acute synovitis had a significantly higher percentage of Leu 3+ cells and a significantly lower percentage of Leu 2+ cells in SF than had patients with RA or other forms of chronic synovitis. In patients with acute synovitis the ratio of Leu 3+ to Leu 2+ cells was significantly higher in SF (4.0) than in blood (1.9) whereas in patients with RA the ratio was significantly lower in SF (1.1) than in blood (2.7). Patients with other forms of chronic synovitis also had a lower Leu 3/Leu 2 ratio in SF (1.1) than in blood (1.9). Two patients with acute synovitis who were retested after 10 days and 10 months respectively (the latter patient having developed definite RA) showed a decrease of the synovial Leu 3/Leu 2 ratio. Thus it appears that the distribution of T lymphocyte subsets in SF was related to the duration of the synovitis.

Acute Disease↗

PubMedDate not supplied

Non-specific polyclonal antibody response induced by Mycoplasma pneumoniae.

The ability of heat-killed Mycoplasma pneumoniae (MP) organisms to induce polyclonal antibody production in cultures of blood lymphocytes of healthy subjects was studied. MP induced both IgM and IgG production, with a predominance of IgM. Supernatants of MP-stimulated lymphocyte cultures were tested by an enzyme-linked immunosorbent assay for antibodies to measles, rubella, and herpes simplex virus. MP as well as pokeweed mitogen induced production of viral antibodies of IgG class in lymphocytes of donors who had serum antibodies to the corresponding viral antigens. The MP-induced non-specific antibody response was T-cell-dependent. Lymphocytes from four patients with MP pneumonia, collected nine to 13 days after onset of illness, were tested for in vitro Ig production in the absence of MP. These lymphocytes spontaneously produced increased amounts of IgM and/or IgG. Lymphocytes from three of these four patients spontaneously produced viral IgG antibodies to measles and/or varicella antigens, indicating that MP had induced non-specific activation of memory B cells in vivo. Spontaneous viral antibody production was not found in lymphocyte cultures of healthy donors. The non-specific activation of blood B cells in vitro is probably induced by non-specific helper factors from MP-activated T cells. It is possible that in vivo MP also may have a direct activating effect on B cells.

Adult↗

PubMedDate not supplied

Whole inactivated SIV vaccine grown on human cells fails to protect against homologous SIV grown on simian cells.

Several groups have reported protection against experimental SIV infection in macaques immunized with a whole inactivated virus vaccine. The aim of the current study was to investigate whether five macaques vaccinated with whole inactivated SIV and previously shown to be protected against challenge with two divergent strains of SIV grown on human cells could resist challenge with a subsequent homologous SIV grown on macaque cells. We show here that this same vaccine did not protect when the challenge virus was grown on primary cells of monkey origin.

AIDS Vaccines↗

PubMedDate not supplied

Anti-actin antibodies of human and rabbit origin.

The production of actin antisera in rabbits was described and their reaction in various immunological tests were compared with the results obtained with human anti-actin sera (smooth muscle antibodies). Even if the sera may react with different antigenic determinants of actin as suggested by results in immunoprecipitation and blocking experiments, the rabbit and human sera can be used for studies of cells with equal or similar results. A prerequisite was that the rabbit anti-actin titres were high. The difficulty in obtaining strong anti-actin sera in rabbits was confirmed.

Actins↗