PubMed Health⌕ Search

Biomedical subjects

G Bolelli

Publications and source records attributed to G Bolelli.

31 records · Page 2Linked to original sources
PubMed1983

Mathematical analysis of antiserum titre and affinity in rabbits injected with 11 alpha OH-progesterone-hemisuccinate-BSA.

A mathematical procedure is presented here to determine the behaviour of antiserum titre in rabbits, after repeated injections of 11 alpha OH-Progesterone-hemisuccinate-BSA. By means of methods recently applied to the time series, the peaks of maximum response were determined. A common behaviour of rabbits was revealed for the delay times in the response after each booster injection. The titre and affinity of antiserum were determined by means of a special method for mathematical treatment of data in RIA analysis, which gives directly such parameters with a statistical interpretation.

Animals↗
PubMed1981

Hormone levels in methadone-treated drug addicts.

To test the effect of methadone on hormone release in man, plasma levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), 17-beta-estradiol (E2), estrone (E1), progesterone (P), testosterone (T), dehydrotestosterone (DHT), androstenedione (A), dehydroepiandrosterone sulphate (DS), and prolactin (PRL) have been evaluated in 25 male subjects in treatment wit 30 mg/day methadone per os for at least two months after a minimum of one year of heroin addiction. All subjects had a history of sexual difficulties. The levels of E1, E2, P and LH were always within normal values whereas FSH showed lower values than normal and A, DS and PRL noticeably increased in many subjects. Modest variations were noted for T and DHT. These data seem to indicate that methadone abuse affects the hypophysis, the testiculum and the adrenal cortex. More details will probably be obtained when the variations in hormone rates and opiate serum concentrations are checked at a later date.

Adolescent↗
PubMed1980

Radioimmunoassay of three oestrogens and three androgens in the same plasma sample after extraction and chromatographic separation.

The plasma levels of oestrone (Oe1), 17 beta-oestradiol (Oe2), oestriol (Oe3), testosterone (T), 5 alpha-dehydrotestosterone (DHT) and androstenedione (A) were assayed by RIA in plasma obtained from peripheral venous blood. The hormones are isolated from the plasma extract, first by Sephadex LH-20 column chromatography (Oe2/Oe3/Oe1, T, DHT, A) and after Oe1, T, DHT, A by TLC on silica gel 60 F254. The accuracy, reproducibility and sensitivity of the method make it satisfactory for clinical studies.

Androgens↗
PubMed1979

Heroin addiction: relationship between the plasma levels of testosterone, dihydrotestosterone, androstenedione, LH, FSH, and the plasma concentration of heroin.

Changes in sexual function and hormone levels are commonly found in subjects addicted to narcotics. In this study we examined 16 male and 3 female addicts who had been taking heroin (H) in the last year in doses higher than 150 mg/day. In these patients, who presented similar clinical problems, we assayed by RIA the plasma levels of heroin, testosterone, (T), dihydrotestosterone (DHT), androstenedione (A), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) for periods of 150 min, 6 h and 9 h. We found a significant reduction of T and DHT concomitant with higher plasma concentrations of heroin but no relevant changes of A, LH and FSH. T and DHT returned to the initial levels after the decrease of heroin concentration. The GnRH test effectd on a female subject allowed us to make the diagnosis of hypothalamic amenorrhea. In the same patient no circadian rhythms for T, DHT and A were detected.

Adolescent↗
PubMed1979

The ultradian pulsatile release of gonadotropins in normal female subjects.

With an original statistical study which employes the analysis of variance and the harmonic analysis, the authors evaluated the levels of LH and FSH obtained from 5 female patients of the infertility clinic. These subjects, apparently free from endocrine disorders, underwent a 7-hour test consisting of samples obtained at 20 minute intervals. By a double-antibody RIA method, we assayed LH and FSH in quintuplicate or triplicate in each sample and counted the tubes twice consecutively. In all subjects for LH and in 3 subjects for FSH we detected a real ultradian pulsatile release of the hormone. Period and amplitude of the LH fluctuations vary according to the phase of the menstrual cycle (follicular phase: short period and low amplitude; luteal phase: long period and high amplitude). No change in amplitude was observed for FSH, while the period in two cases varied in accordance with LH. In two cases we found multiple periods in the fluctuations of one hormone during the same test.

Adult↗
PubMed1979

Plasma levels of unconjugated estetrol and estriol and of total estriol in normal human pregnancy.

The course of normal pregnancy in 54 patients was monitored by weekly assays of Unconjugated Estriol (E3U), Total Estriol (E3T) and Unconjugated Estetrol (E4U). These subjects were divided into two groups: one of those patients who delivered a fetus with a weight above the 50th percentile and the other of those who delivered a fetus with a weight below the 50th percentile. No significant difference was found between these two groups and it is not therefore possible to have information regarding the weight of the fetus, starting from the weekly values of these hormones. Analogous variations of the three hormones were found as pregnancy progresses. However, the rate of increase for E4U was higher than for E3T and E3U.

Adolescent↗
PubMedDate not supplied

Radioimmunoassay of morphine and morphine-like substances in biological fluids and human tissues.

A radioimmunoassay is described for the determination of morphine and morphine-like substances in plasma, serum, biological fluids and tissue homogenates using an antiserum to morphine-6-hemisuccinyl-BSA and 125I-morphine as the labelled tracer. In the B/F separation with ammonium sulphate, calcium sulphate was added to make the precipitate more compact. Some parameters related to the use of this method in direct assay on plasma, serum, other biological fluids and tissue homogenates were evaluated.

Animals↗
PubMedDate not supplied

Gonadal and adrenal secretion of dehydroepiandrosterone sulfate in prepubertal and pubertal subjects.

The Authors have evaluated the relationship between the secretion of dehydroepiandrosterone sulfate (DHA-S) by the adrenal glands and by the gonads in a group of prepubertal and pubertal subjects ("short normal"), both males and females. In the male subjects of a hCG test and an ACTH test were performed; in the female subjects only the latter test was carried out. The behavior of DHA-S under basal conditions was also assessed in both sexes and related to bone age and chronological age in the prepubertal period and during the early stages of puberty. Plasma levels of DHA-S in both sexes increase progressively with chronological age and bone age. A negative correlation was found between DHA-S and bone delay (expressed in percent relative to chronological age) in prepubertal subjects, both males and females. A significant increase in DHA-S after hCG stimulation was found both in prepubertal and pubertal boys. After ACTH stimulation DHA-S increased significantly in prepubertal and pubertal males and females; throughout the test no difference was found between prepubertal and pubertal subjects nor between male and female subjects. Our data confirm that DHA-S is produced both by the adrenals and by the testes.

Adolescent↗
PubMedDate not supplied

Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma.

Prospective studies based on the storage of biological samples at low temperature have opened new perspectives in etiological research on cancer. In planning these studies a crucial question is to evaluate whether the long-term preservation of samples is able to affect the categorization of the subjects involved. In the frame of the ORDET project, a prospective study of hormones and diet in the etiology of breast cancer provided with a -80 degrees C biological bank, we have evaluated the stability of estradiol, free and total testosterone, and prolactin in serum and plasma samples over 3 years of cryoconservation. Study results showed that the subjects maintained almost the same rank by hormonal concentration throughout the 3-year period for all hormones. Looking at the stability over time, estradiol, prolactin, and total testosterone had fairly good performance for both serum and plasma. Serum-free testosterone increased in time up to 30%, whereas progesterone decreased by about 40% of the initial concentration. However, the reliability of the individual categorization by hormonal level suggests the validity of low temperature storage for epidemiological purposes, at least for hormonal parameters.

Blood Preservation↗