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Biomedical subjects

G Brandner

Publications and source records attributed to G Brandner.

15 recordsLinked to original sources
PubMed1988

Synthetic oligopeptides define epitopes at the amino- and carboxy-terminus of simian virus 40 large tumor antigen which are recognized by monoclonal antibodies.

Synthetic oligopeptides were used to define the epitopes recognized by monoclonal antibodies directed against the SV40 large tumor (T) antigen. The two monoclonal antibodies PAb 1622 and PAb 1626 reacted with the N-terminal undecapeptide Met(1)-Leu(11) as detected by inhibition of nuclear SV40 T antigen immunofluorescence. Antibody PAb 1605 recognized the hexapeptide Thr(701)-Pro(706) at the carboxy-terminus of the SV40 T antigen as detected by inhibition of an enzyme-linked immunosorbent assay. The specificities of these monoclonal antibodies differ from those of antibodies previously raised against the respective synthetic oligopeptides.

Antibodies, Monoclonal↗
PubMed1988

Interferon inhibits herpes simplex virus-specific translation: a reinvestigation.

Reports on the arrest of herpes simplex virus type 1 (HSV-1) replication by interferon (IFN) are inconsistent. By the use of immunofluorescence and immunoblot assays with monoclonal and polyclonal antibodies, effective arrest of viral translation by human IFN-alpha in human fibroblasts was detected for the HSV-1 strains KOS and McIntyre. In HeLa cells which are less sensitive to IFN inhibition and in 444 cells, a HeLa-fibroblast hybrid cell line, the inhibition was less pronounced. These results confirm earlier observations that IFN or polyinosinic.polycytidylic acid block the replication of HSV-1 in human, monkey and mouse cells no later than the immediate early phase of infection.

Animals↗
PubMed1979

Simulation and prevention of retrovirus--specific reactions by mycoplasmas.

From human mycosis fungoides tumor-derived cell lines, Mycoplasma hyorhinis was isolated. This mycoplasma shared the following characteristics with retroviruses: uptake of 3H-uridine, but not of 3H-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3H-TMP into high molecular weight material in standard reverse transcriptase assays with the template-primer poly (A) . (dT)12. On the other hand, the specific reverse transcriptase reaction of retroviruses with poly(A) . (dT)12 and poly(C) . (dG) approximately 16 was almost completely abolished in the presence of the mycoplasma. Thus, M. hyorhinis may interfere with identification and isolation procedures for retroviruses.

Cell Line↗
PubMed1977

Isolation of simian virus 40 from a newborn child.

Rubella virus and simina virus 40 (SV40) were isolated from a newborn child suffering from neurological and anatomical anomalies. The SV40 isolate was very similar to SV40 strain 777 by electron microscopic, biological, and immunological criteria.

Abnormalities, Multiple↗
PubMed1975

Cytosine arabinoside- and interferon-mediated control of polyoma and SV40 genome expression.

By metabolic DNA inhibitors such as araC, viral as well as host DNA replication is suppressed in polyoma- and SV40-infected cells. The interruption of the current viral DNA replication has no effect on the current transcription of the late viral genes. The persistence of the late transcription indicates that the onset, but not the persistence, of the viral DNA replication is a prerequisite for the persistence of the late polyoma and SV40 genome transcription. Pretreatment of monkey kidney cells with poly(I):-poly(C) nearly completely inhibits the SV40 T antigen formation; the early SV40 RNA formation is suppressed far less. This type of SV40 genome control favors the concept of a primary action of poly(I):poly(C)-mediated interference on SV40 translation.

Cell Line↗
PubMedDate not supplied

Loading of human red blood cells with DNA and RNA.

Human erythrocytes were suspended in Hank's solution containing mammalian or viral DNA or RNA. After dialysis at 0 degrees C first against water and subsequently against Hank's solution, and a further incubation at 37 degrees C, the erythrocytes were found to be loaded with the nucleic acids. The nucleic acid trapped in the erythrocytes exhibited up to 35 per cent of the external concentration.

Cell Membrane Permeability↗
PubMedDate not supplied

African green monkey fibroblast actin morphology during SV40 infection.

Monkey skin fibroblasts were infected with simian virus 40. Cells that exhibited the viral tumor antigen were found to retain the normal morphology of actin filaments up to six days after infection. However when cells were transformed in terms of focus formation they had lost the normal actin morphology.

Actins↗
PubMedDate not supplied

Transformation-related cellular protein p53: increased level in untransformed rat cells following treatment with the tumorpromoter, tetradecanoylphorbol-acetate.

12-O-Tetradecanoylphorbol-13-acetate (TPA, 100 ng ml-1), a tumor promoting phorbol ester, is able to induce enhanced levels of the transformation-associated cellular antigen p53 in normal rat 2 cells which had not been previously initiated by a carcinogen. p53 was estimated in ethanol-fixed treated cells on microtiter plates with ELISA using the monoclonal antibody Pab 1620 [EMBO J. 7, 1485, (1984)]. Induction of p53 was confirmed by immunoblotting. This effect of TPA is an additional phenotypic characteristic of tumor cells which can be induced by TPA in untransformed rodent cells.

Animals↗