Biomedical subjects

G Brucke

Publications and source records attributed to G Brucke.

2 recordsLinked to original sources

PubMed1980

Concanavalin A capping in polymorphonuclear leukocytes.

Various polymorphonuclear leukocyte (PMN) functions are dependent on an intact intracellular cytoskeleton consisting of the microtubules and the microfilaments. To investigate the microtublule system in PMNs we observed the spontaneous, Colchicine and Diamide induced cap-formation by fluorescence microscopy ion PMNs obtained from children with bacterial and viral infections demonstrated with 47 +/- 1% a significantly increased number of spontaneous capped PMNs compared to 22 +/- 1% capped cells obtained from controls. Furthermore, 52 +/- 2% PMNs of patients on immunosuppressive therapy exhibited spontaneous surface capping. There was no significant elevation in the number of capped PMNs (30 +/- 2%) obtained from children with viral infections. Colchicine and Diamide increased the number of capped cells in control PMNs as well as in PMNs from patients to 69 +/- 1% and 67 +/- 1%, respectively. Since the increased spontaneous cap formation in PMNs is associated with a defect of microtubule assembly, the various leukocyte function defects described in patients with bacterial infections, bronchial asthma or on immunosuppressive therapy may have to be considered the consequence of an altered microtubule system.

Asthma↗

PubMedDate not supplied

[Changes in the granulocyte membrane in mucoviscidosis].

Important polymorphonuclear leukocyte (PMN) functions are dependent on an intact cytoskeleton, consistent of the microtubulus and microfilament system. This can be assayed from the mobility of fluorescence labelled Concanavalin A (Con A) receptor complexes on the cell surface. Con A shows an uniform surface distribution on cells with an intact microtubulus system, whereas it's disruption causes a Con A cap formation. The alteration of the microtubulus and microfilament systems induces a patchy Con A distribution. Only 44% PMNs of 20 children with cystic fibrosis exhibited with a random Con A fluorescence distribution an intact cytoskeleton compared to 69% control PMNs. But 32% cystic fibrosis PMNs and 23% control PMNs showed a Con A capping phenomena, which is associated with a microtubulus alteration. In addition, 24% cystic fibrosis PMNs demonstrated a patchy fluorescence, representing a microtubulus and microfilament disruption, compared to 8% patched control PMNs. This study supports the idea, that the chronic bacterial infections cause in cystic fibrosis PMNs a cytoskeleton defect, which represents as a leukocyte-function defect an additional infection promoting factor.

Adolescent↗