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G Bucci

Publications and source records attributed to G Bucci.

At least 37 records · Page 2Linked to original sources
PubMed1989

Fine structure of the excretory system of the deep posterior (Ebner's) salivary glands of the human tongue.

Human deep posterior lingual glands (von Ebner's glands) are located beneath the circumvallate papillae. They are formed by tubuloalveolar adenomeres, intercalated ducts and excretory ducts coming together in the main excretory duct. The tubuloalveolar cells, pyramid-shaped, show large and dense secretory granules (clear cored) throughout the cytoplasm, rare basal folds and packed cisternae of rough endoplasmic reticulum (RER) at the basal pole. The columnar cells of the intercalated ducts are arranged in a monolayer. They are characterized by dense, clear-core secretory granules (mostly in the apical cytoplasm), a basal nucleus, well-developed RER and Golgi apparatus, and thin filaments distributed in supra- and perinuclear cytoplasm. Striated ducts are absent. Excretory ducts, coming together in the main duct, are lined by a bistratified epithelium. The inner layer consists of columnar cells showing bundles of tonofilaments with scarce secretory activity. The outer layer is composed of basal cells lying on the basal lamina. The main excretory duct, which opens at the bottom of the vallum, shows a stratified epithelium. The outer side is composed of 2-3 layers of malpighian cells lying on the basal lamina. The inner side consists of a single layer of cuboidal-columnar cells with dense apical granules and well-developed organelles synthesizing and condensing secretions. These cells interpolate with goblet cells, rare mitochondria-rich cells, ciliated cells and numerous small globous cells showing a clear matrix and lacking secretory granules. The cilia show a 9 + 2 microtubular structure with basal bodies provided with striated rootlets. Myoepithelial cells surround with their processes the basal portions of the secretory cells and the intercalated ducts. The conclusions concern some comparative aspects and some hypothesis on the functional role of goblet cells, ciliated cells and epithelial cells lining the different ducts, also in relation to the final secretory product.

Aged↗
PubMed1989

Listeria SPP. and enteric pathogens in raw meat: a survey in the Ferrara area.

The Authors report the results of a study performed on 113 samples of raw meat of 6 different animal species in order to evaluate the extent of contamination by some organisms responsible for food-borne infections. A total of 2 strains of Salmonella (1.7%), 36 of Yersinia (31.8%), 35 of Campylobacter (30.9%) and 13 of Listeria (11.5%) were isolated. The possible role of contaminated meat in cases of human listeriosis is pointed out.

Animals↗
PubMed1989

Fine structure of bat deep posterior lingual glands (von Ebner's)

We studied the morphology and ultrastructure of the bat (Pipistrellus k.k. and Rhinolophus f.e.) deep posterior lingual glands (Ebner's glands) during hibernation, summer and after stimulation with pilocarpine. Ebner's glands are formed by serous tubulo-alveolar adenomeres and by an excretory system organized in intercalated ducts, long excretory ducts and a main excretory duct. The latter opens in the vallum which surrounds the circumvallate papillae and in the groove of the foliate papillae. The secretory cells, which lack basal folds, show abundant and dense granules (PAS+, Alcian blue -), microvilli (scarce during hibernation), a Golgi apparatus (well developed during summer and after stimulation with pilocarpine), a large nucleus and RER cisternae stacked at the basal pole. Centrioles, lipid droplets, heterogeneous bodies (in content and density, probably lipofuscin bodies), lysosomal multivesicular bodies and large, dense granules with a microcrystalline structure were also encountered. The lateral membranes of adjacent cells are joined by desmosomes; their interdigitations are neither numerous nor prominent during summer. Microfilaments, often gathered in small bundles, lie in the lateral, peripheral cytoplasm without any relation with desmosomes. In summer and particularly after stimulation with pilocarpine, the apical pole of the secretory cells is characterized by many long microvilli, pedunculated hyaloplasmic protrusions and secretory granules. During hibernation the lumen is filled with secretory material. Myoepithelial cells are arranged among secretory cells or between them and the basal lamina. The short intercalated ducts show similarities with the analogous ducts of the parotid gland. Striated ducts are absent. Excretory ducts are endowed with: a) an inner layer of cuboidal cells characterized by poorly developed cytoplasmic organelles, rare dense granules and a few small microvilli; b) an outer layer of basal cells lying on the basal lamina. Myoepithelial cells are absent. The main excretory duct is lined by a stratified epithelium with an inner layer of conical-pyramidal cells surrounded by two-three rows of basal cells. The conical-pyramidal cells show poorly developed organelles, an apical border with small short microvilli and a prominent terminal web.

Animals↗
PubMed1988

Ecological survey of Listeria in the Ferrara area (northern Italy).

A bacteriological survey was done on human and environmental specimens from the province of Ferrara in Northern Italy to determine the prevalence of Listeria spp. because no cases of listeriosis so far had been reported. Ten strains of Listeria spp. (1.9%) (7 L. monocytogenes, 3 L. innocua) were detected in 513 faecal specimens from asymptomatic humans, whereas none was recovered from 505 vaginal swabs of fertile women with discharges. Thirteen strains (9 L. monocytogenes, 4 L. innocua) were detected in 113 meat samples from retail outlets (11.5%), 4 strains (2 L. monocytogenes, 2 L. innocua) from 75 samples of frozen food (5.3%). Eleven Listeria strains (1 L. monocytogenes, 2 L. seeligeri, 1 L. welshimeri, 7 L. innocua) were isolated from 50 water samples of the river Po (22.0%); 15 strains (4 L. monocytogenes, 11 L. innocua) from 80 samples of other surface waters (18.8%); 1 strain (L. innocua) from 98 samples of groundwaters (1.0%); 14 strains (8 L. monocytogenes, 5 L. innocua, 1 L. seeliger) from 33 samples of urban sewage (42.4%). Only 35 (51.5%) of the Listeria isolates could be phage-typed. Analogies were observed between the distribution of Listeria and that of Salmonella, Yersinia and thermophilic Campylobacter in some materials in which these organisms were sought simultaneously.

Animals↗
PubMed1988

The ultrastructure of the thyroid parafollicular cells of the marmot (Marmota marmota). Immunocytochemical localization of calcitonin and somatostatin.

We studied the topographical and ultrastructural characteristics of the parafollicular cells in the Marmota marmota and their seasonal changes. The parafollicular cells, together with the follicular cells, form the follicular epithelium occupying a peculiar basal position. Furthermore these cells are characterized by a clear cytoplasmic matrix and well developed cytoplasmic organelles. The latter synthesize and condense the secretory product which ultrastructurally is organized in secretory granule-vesicles with a scarcely electron-dense cottony content. Histochemically the secretion consists in a proteic complex (rich in alpha-amino acids and sulphonate proteins) with a minor glucidic component. The above metabolic activity closely relates with the seasonal metabolic changes and namely with calcium metabolism. The immunocytochemical reactions revealed the presence of calcitonin and, to a lesser extent, somatostatin. The morphological findings were compared with those observed in other hibernating mammalians and related to the calcium homeostasis.

Animals↗
PubMed1988

[The hypothalamic-hypophyseal neurosecretory system of the Alosa fallax nilotica. A histological and ultrastructural study].

We studied the morphological, histochemical and ultrastructural aspects of the hypothalamic-pituitary system in the Alosa fallax nilotica. The secretory activity of the preoptic nucleus and of the lateral tuberal nucleus was also investigated with regard to the gonadal growth and development during the reproductive sexual cycle and the changing migrational environmental conditions (anadromous and catadromous phases). The hypothalamic-pituitary system is formed by: centers of neurosecretion synthesis (parvo and magnocellular preoptic nucleus and lateral tuberal nucleus), a conduit system (preoptic infundibular-neurohypophyseal tract) and a collecting center (neurohypophysis intermediate lobe). The neurosecretory cells show two distinct types of secretory granules with different ultrastructural features probably representing the morphological expression of two distinct hormones. These cells show also characterizations of cytoplasmic areas related to peculiar cyclic functional stages concerned with the reproductive sexual cycle. We observed significant quantitative and qualitative changes of the neurons both in the parvo- and magnocellular preoptic nucleus and in the lateral tuberal nucleus during anadromous migration and during spawning and fertilization. Almost insignificant changes of the neurosecretory material were observed in the preoptic-pituitary tract and in the intermediate-neurohypophyseal lobe. The results show that the hypothalamic-pituitary neurosecretory system play an important role in the osmoregulation (magnocellular preoptic nucleus) and on the reproductive cycle (lateral tuberal nucleus). The system probably also controls some other metabolic activities (utilization of the stored material) linked to peculiar environmental and feeding conditions.

Animals↗
PubMed1988

[Isolation in the Ferrara province of Campylobacter thermophile from man, animals, food and the environment during the 2-year period 1984-1985].

During a two-years period (1984-1985), thermophilic campylobacters were sought in materials of various origin: isolation rates were as follows: from 1680 stool specimens of enteropathic patients, 6.0%; from 1920 stool specimens of asymptomatic subjects, 0.5%; from 325 stool specimens of healthy animals, belonging to 5 different species, 51.7%; from 106 meat samples of 6 different species, 32.1%; from 194 samples of raw milk, 0.5%; from 43 samples of surface waters, 37.2%; from 150 samples of waste water, 4.0%. 286 Campylobacter strains were typed according to the Skirrow's scheme: C. jejuni biot. 1 represented 64.0% of isolates, followed by C. coli (29.7%), unclassified Campylobacters (5.6%), C. fetus (0.3%) and C. jejuni biot. 2 (0.3%).

Animals↗
PubMed1988

Topography and ultrastructure of kidney lymphatics in some hibernating bats.

The kidney lymphatic system of some bats consists of intraparenchymal (interlobar, arcuate, and interlobular) and extraparenchymal vessels (capsular and prehilar connective). These vessels drain lymph via precollecting and prenodal collecting lymphatics into a hilar lymph node. There are no lymphatics in the renal medulla. The lymphatic vasculature (precollecting vessels excluded) is characterized by an endothelial wall lacking basal lamina and fenestrations. The endothelial cells, mostly rectangular in shape, are joined together by overlapping, end-to-end, and complex interdigitating junctions. Cytoplasmic expansions profile and thickness, intercellular junctions and particularly the different categories of uncoated vesicles (free or opened on luminal or abluminal surface) show qualitative and quantitative seasonal variations. Luminal and abluminal cytoplasmic processes appear (when analyzed in tridimensional reconstructions) as "intraendothelial channels." The increased number of these structures during summer characterizes them as dynamic elements and supports the concept of an active role played by them in transendothelial transport. Nevertheless, the main functional role is still ascribed (in addition to membrane transport mechanisms) to the vesicular system, also defined as the "vesicular route." We did not find any open intercellular junctions.

Animals↗
PubMed1986

[Isolation of Listeria from the water of the Po River].

Bacteria belonging to the genus Listeria were isolated from samples of water of the river Po, using the following methods: enrichment: 1000 ml of water were filtered through 0.45 micron membrane filters, which were in turn immersed in a tube containing 50 ml of tryptose phosphate broth and subsequently incubated at 4 degrees C up to 60 days. isolation: 1 ml from the above enrichment was subcultured fortnightly in 9 ml of nutrient broth supplemented with potassium thiocyanate (3.75% w/v) and nalidixic acid (100 mg/l). After a 48 h incubation at 37 degrees C, a loopful of this culture, diluted 1:10 with saline, was streaked on to a tryptose agar plate, which was then incubated at 37 degrees C for 48 h. Colonies showing a blue-green iridescence when observed with oblique transillumination were submitted to the usual identification tests. Organisms which proved to belong to the genus Listeria were sent to the Centre of Bacterial Ecology of the Pasteur Institute (Paris) and, in some cases, to the Institute of Hygiene, University of Würzburg, GFR, for further characterization (species, phagovar, serovar). This technique allowed us to isolate Listeria strains from 11 out of 50 water samples (22%). The organisms were distributed among the following species: L. monocytogenes (1 strain), L. innocua (7 strains), L. seeligeri (2 strains), L. welshimeri (1 strain). In spite of the limited number of isolates, the great variety of species identified has to be pointed out. Furthermore, one strain belonged to L. welshimeri, a new species which is, to our knowledge, rarely found in Europe, while it seems more common in U.S.A.

Listeria↗