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Biomedical subjects

G Burnett

Publications and source records attributed to G Burnett.

8 recordsLinked to original sources

Pregnancy discovery and acceptance among low-income primiparous women: a multicultural exploration.

OBJECTIVES: As part of a larger study exploring psychosocial factors that influence self-care and use of health care services during pregnancy, we investigated the process of pregnancy discovery and acceptance among a culturally diverse group of women who had given birth to their first child in the year preceding data collection. METHODS: Eighty-seven low-income women from four cultural groups (African American, Mexican, Puerto Rican, and white) participated in eight focus groups held in their communities. The focus groups were ethnically homogenous and stratified by early and late entry into prenatal care. A social influence model guided the development of focus group questions, and the study followed a participatory action research model, with community members involved in all phases of the research. RESULTS: Issues that emerged from the focus groups as possible influences on timing of pregnancy recognition include the role of pregnancy signs and symptoms and pregnancy risk perception in the discovery process, the role of social network members in labeling and affirming the pregnancy, concerns about disclosure, "planning" status of the pregnancy, and perceived availability of choices for resolving an unintended pregnancy. CONCLUSIONS: The pregnancy discovery process is complex, and when protracted, can potentially result in delayed initiation of both prenatal care and healthful pregnancy behaviors. Enhancing our understanding of pregnancy discovery and acceptance has clear implications for primary and secondary prevention. Future research is needed to further explain the trajectory of pregnancy discovery and acceptance and its influence on health behaviors and pregnancy outcome.

Adolescent↗

Remote communication from a mobile terminal: an adjunct for a computerized intensive care unit order management system.

OBJECTIVES: To develop and implement a fully mobile computer terminal that interfaces with our computerized intensive care unit (ICU) local area network and order management system. This system can provide access to the entire network and to order review and entry and during ICU bedside rounds. DESIGN: Descriptive report. SETTING: Surgical ICU in Department of Veterans Affairs Medical Center. SYSTEM CONFIGURATION: A parallel local area network was configured for the remote mobile computer system. A proprietary remote transmission system (Altair II, Motorola) was used. This high-throughput system minimizes interference and errors by using licensed, nonshared, radiofrequency spectra. CONCLUSION: The resulting mobile system is an economical and time-efficient adjunct to an established ICU computerized network and order management system. Clinical working bedside rounds are now routinely conducted with the mobile terminal, providing immediate access to full network resources.

Computer Terminals↗

Food preference following acute or chronic chlordiazepoxide administration: tolerance to an antineophobic action.

Chlordiazepoxide (CDP) at 15 mg/kg produced two distinct actions in a food preference test, firstly a general appetite-enhancing effect, and secondly an anti-neophobic effect. Following acute injection of CDP the rats changed from eating predominantly familiar food to a novel food. This may signify an anti-neophobic effect of CDP. However, following 10 days of treatment with CDP, the anti-neophobic effect was abolished and the choice of familiar food was enhanced. This could be an indication of a more general appetite-enhancing effect. Hence some form of tolerance may develop to CDP's effects over 10 days of treatment which selectively abolishes anti-neophobic action whilst leaving the appetite effect further enhanced. There were no indications of tolerance developing to the actions of CDP in animals familiarized with all the test foods before the preference test was run. Hence the presence of food novelty may be critical to the observation of some form of selective tolerance.

Animals↗

Mechanism-based inactivation of pig heart L-alanine transaminase by L-propargylglycine. Half-site reactivity.

L-Alanine transaminase (EC 2.6.1.2) from pig heart was found to be a dimer, with a subunit molecular weight of 55,000 and one pyridoxal phosphate bound/subunit. Seven free sulfhydryl groups/subunit were detected. Isoelectric focusing revealed three species (native pI values, 5.3 to 5.5). L-Propargylglycine was found to inactivate the enzyme at 37 degrees C with a KI = 3.9 mM and an observed maximal first order rate constant, kinact = 0.26 min-1. Incorporation of 1 [14C]propargylglycine molecule/dimer leads to greater than 97% inactivation, suggesting half-site reactivity, while the unalkylated subunit is still apparently capable of processing L-alanine, L-propargylglycine, and beta-chloro-L-alanine. The minimal stoichiometric ratio necessary for inactivation was determined to be 2.7 L-propargylglycine molecules/enzyme subunit, 2.2 molecules/subunit undergoing transamination before inactivation ensues. A deuterium kinetic isotope effect of 3.5 was observed for inactivation with DL-[2-2H]propargylglycine.

Alanine Transaminase↗

Studies on the kinetics and stoichiometry of inactivation of Pseudomonas omega-amino acid:pyruvate transaminase by gabaculine.

A homogeneous pyruvate-requiring omega-amino acid transaminase from Pseudomonas species F-126 has been examined for its behavior with gamma-aminobutyrate (GABA) as omega-amino acid substrate and for its susceptibility to the cyclic dihydroaromatic GABA analogue, gabaculine, a known suicide substrate for alpha-ketoglutarate-requiring GABA transaminases (Biochemistry 16, 4604-4610, 1977). One isomer of DL-gabaculine serves as a completely efficient titrant (no product molecules released) for this omega-amino acid transaminase by the anticipated mechanism of bound pyridoxal 5'-phosphate (PLP) derivitization. Stoichiometric titration with [2-3H]gabaculine reveals full inactivation at 0.45 labels/enzyme tetramer (see below), consistent with the subsequent demonstration that there is only 0.45 PLP molecule, bound as phenylhydrazine-titratable aldehyde form, per tetramer. Spectroscopic monitoring of inactivation also agrees with formation, on full inactivation, of 0.45 mol of m-anthranilyl-PNP adduct as the species quantitatively responsible for enzyme inactivation. Incubation of enzyme with excess PLP at 60 degrees C allows loading of enzyme with coenzyme to an average level of approximately 1 PLP/subunit, but even in this case activity is only increased up to 1.5-fold, and 1 to 1.5 molecules of gabaculine/tetramer cause complete inactivation. These data may indicate negative cooperativity between subunits.

Cyclohexanecarboxylic Acids↗

Timed ammonia release for steers.

Slow and rapid ruminal ammonia release rates were simulated by intermittent urea feeding with steers fed winter range grass. Four 360-kg, ruminally-cannulated steers were fed 208 g prairie hay (2.56% crude protein) hourly with the following daily dietary supplements: (1) Urea provided continuously (C) at a rate of 3.5 g/hr for 24 hr; (2) urea provided moderately (M) at a rate of 14.2 g/hr for 6 hr (3) urea offered rapidly (R) as 85 g in 1 hr, or (4) no supplemental urea (O). Ruminal ammonia remained stable with treatments C and O at 12.8 and 1.6 mg/dl, respectively. Treatments R and M peaked 1.5 and 6.5 hr after the start of urea feeding at 41.8 and 37.1 mg/dl, respectively. Nonammonia nitrogen concentrations in the rumen were increased by an average of 23% with urea supplementation. Dry matter digestibility was increased by 5% with addition of urea, regardless of the rate of urea administration. Daily nitrogen retentions were 12.4, 12.2, 11.4 and -7.1 g (P < .01) for C, M, R and O, respectively. Simulated slow ammonia release rates enhanced neither dry matter digestibility nor nitrogen retention. Metabolic use of ammonia in the rumen of steers fed forage was not improved by an attenuated release rate.

Ammonia↗