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Biomedical subjects

G C Balboni

Publications and source records attributed to G C Balboni.

At least 37 records · Page 2Linked to original sources
PubMed1986

Erythropoietin levels in heterozygous alpha-thalassemia.

In guinea pig bone marrow cultures with heterozygous alpha-thalassemic serum, 59Fe uptake values are elevated above iron values of cultures with serum of normal subjects. These results show that erythropoietin (EP) activity values in heterozygous alpha-thalassemia are comparable to those previously observed by ourselves in heterozygous beta-thalassemia despite of the different Hb concentration in these thalassemic syndromes. This points to the existence of signals which regulate Ep synthesis independently of Hb levels.

Adolescent↗
PubMed1985

[Effect of laser irradiation on collagen production by fibroblasts in vitro].

The effects of the low power laser irradiation (Space Mix 5 Mid Laser) on the 3H-proline incorporation in the collagenic proteins produced by two lines of normal human fibroblasts in vitro were studied. The 3H-thymidine incorporation in cultures of control and irradiated fibroblasts of the same lines was also evaluated. The obtained results show that in the experimental conditions considered, laser irradiation may have a positive effect on the production of collagen by the fibroblasts in vitro. This effect is dependent on the time of exposure, the frequency of infra-red impulses and the age of subjects from which fibroblasts were obtained. The laser irradiation in the same experimental conditions does not affect the incorporation of 3H-thymidine in the fibroblasts in vitro. Therefore, the observed increase of collagen production is not dependent on an increase of cellular population.

Aged↗
PubMed1984

Erythropoietin levels in heterozygous beta-thalassemia.

In the present work the capacity of serum of beta-thalassemic heterozygous and normal subjects to stimulate 59Fe uptake into heme in guinea pig bone marrow cultures is analyzed. The results show that labelled iron uptake is higher in cultures with thalassemic serum than in cultures with normal serum. No correlation between labelled iron uptake and Hb level or sex has been found in either group.

Adult↗
PubMed1983

[Relaxin in ovarian follicles].

Since the first observations of HISAW, the knowledge on the chemistry and the biological properties of relaxin improved in an important way. It is now well known that relaxin is a peptide hormone, strictly related to insulin. The extraction and purification of porcine relaxin and the application of radio-immunological and immuno-histochemical methods have provided a lot of data concerning the activity and the sites of production of the hormone. Recently human relaxin was synthesized. Besides the well known effects on the pubic symphysis and the uterus further actions were detected in the ovary, in the mammary gland, in the seminal plasma etc... Relaxin is produced not only by the corpus luteum, as at first believed, but also by decidua, endometrial glands and, in the male, by the prostatic gland. Some findings suggest a possible production by follicular cells too. In fact, relaxin was detected in the follicular fluid and the production of the hormone by porcine granulosa cells was demonstrated in vitro. Our immuno-histochemical research has shown the presence of relaxin-like substances in the granulosa cells of cavitary follicles of the human and porcine ovary.

Animals↗
PubMed1982

[Dark cells of the rat olfactory epithelium].

Two types of basal cells can be identified in the olfactory epithelium: a) the clear basal cells, round in shape, which are true stem cells capable of giving rise to the olfactory and the supporting cells; b) the dark basal cells characterized by a very irregular shape and by the presence in the cytoplasm of numerous mitochondria and bundles of microfilaments. In the aim of interpreting the significance of these dark cells we studied them with the light and electron microscope in the olfactory epithelium of male and female prepubertal and postpubertal rats. The nature of the microfilaments was investigated with the indirect immunofluorescence method for prekeratin and actin. The results show that the basal cells modify their morphology in different endocrine conditions and that microfilaments are made up by actin. Therefore, in our opinion, these cells must be considered as a particular type of supporting cells, capable of directing the cellular movement and arrangement in the olfactory epithelium, when an active cellular turnover occurs.

Aging↗
PubMed1981

[Behaviour of thymus mast cells in the rat under various experimental conditions].

The distribution and the histochemical characteristics of the mastocytes of the thymus of postpuberal rats have been studied, in different experimental conditions (control, castrated, castrated and treated with testosterone propionate). The obtained results may be summarized as follows: 1) The mastocytes are located in the thymic capsule and in the connective septa. Among the parenchymal cells, mastocytes have been never observed. 2) In the thymus of castrated rats the total number of mastocytes is statistically significantly reduced. 3) In the thymus of the rats castrated and treated with testosterone, the total number of the mastocytes, in comparison to the castrated rats, increases in a statistically significant way. 4) No evidence has been found of a genesis of mastocytes from the parenchymal thymocytes.

Animals↗
PubMed1981

[Mast cells in rat lymph nodes].

The histochemical and ultrastructural features of mastocytes have been studied in rat parathymic, para-aortic, inguinal and mesenteric lymph nodes. 1) In the parathymic lymph nodes, as well as in the lymph nodes of other sites in the body, all the stages of evolution of mastocytes may be detected, related to a progressive sulfation of the granular mucopolysaccharides. 2) It can be suggested that in the rat lymph nodes a genesis of mastocytes, probably from undifferentiated mesenchymal elements, might occur. No evidence have been found of a possible origin of mastocytes from lymphocytes. 3) The number of mastocytes and the extent of process of maturation of these cells in quite variable in relation to the different functional conditions of the lymph nodes.

Animals↗
PubMed1981

On the granulosa cells of ovarian follicles. I. Morphometric research on the relationship between granulosa and theca interna in evolutive follicles.

In the aim of detecting some morphological relationship between granulosa and theca interna layers in evolutive follicles of different mammalian species, a morphometric research has been carried out. In the follicles, classified according to their size, the thickness of the thecal layer has been measured at the pole of the follicle corresponding to cumulus oophorus and at the opposite side. The obtained results have been statistically analyzed. At the level of the cumulus oophorus the thickness of the theca interna layer results to be higher than in the opposite side during all the life-span of the evolutive follicles.

Animals↗
PubMed1981

On the granulosa cells of ovarian follicles. II. Identification of different morphological patterns of granulosa cells in evolutive follicles.

An attempt has been made for identifying different types of granulosa cells in the wall of cavitary ovarian follicles. Human, porcine and rat ovaries have been examined at the light and electron microscopes. Some smears of granulosa cells as well as human foetal ovaries have been also studied. These preliminary results seem to confirm that in the granulosa layer of evolutive follicles the cells may present some different morphological and histochemical features.

Animals↗
PubMed1981

On the structural changes of granulosa cells cultured in vitro. Histochemical, ultrastructural and stereological observations.

Granulosa cells from porcine ovarian follicles were cultured in vitro in standard medium and in medium supplemented with HCG. After culture the cellular 3 beta HSD enzymatic activity was evaluated and the cells were studied by TEM and SEM. A stereological evaluation of the smooth and rough endoplasmic reticulum was carried out. The results indicate that the cultured cells undergo a luteinization process which is more evident in the presence of HCG. In fact, in comparison with the controls, in these cells the enzymatic activity is higher, the rough endoplasmic reticulum decreases and the smooth endoplasmic reticulum increases, the cytoplasm shows lipid droplets and vesicular mitochondria. The cell surface develops a number of microvillus-like evaginations.

Animals↗
PubMed1980

[Mastocytes in the rat thymus].

The distribution and the histochemical characteristics of the thymus mastocytes of postpuberal rats have been studied, in different experimental conditions (control, castrated, castrated and treated with testosterone, and fatigued rats). The results may be summarized as follows: 1) Mastocytes have been found only in the thymic capsule and connective tissue septa : they have never been observed among the parenchymal cells. 2) In the thymus of castrated rats the total number of mastocytes is significantly reduced. 3) In the thymus of the rats castrated and treated with testosterone, the total number of mastocytes, in comparison to the castrated rats, increases in a statistically significant way. A slight increase was observed also in comparison to the controls. 4) In the thymus of the fatigued rats a statistically significant increase of the number of mastocytes has been observed. 5) In the sinuses of the parathymic lymphnodes, numerous mastocytes and some cells that for their histochemical characteristics can be regarded as immature mastocytes, have been found.

Animals↗
PubMed1980

[Role of the mastocytes during functional activation of the thyroid].

The behaviour of biogenic amines in mastocytes has been studied in the thyroid glands of rats treated with 2 U.I. of TSH. The histochemical evaluation of histamine and 5-HT in mastocytes with the method of fluorochromization has been carried out at 20 minutes and 120 minutes after TSH administration. After 20 minutes, the thyroid glands do not present evident parenchymal changes, but the positivity for biogenic amines in mastocytes decreases in comparison to the controls, while this positivity begins to be evident in the interstitial tissue. After 120 minutes, the thyroid gland shows a microfollicular arrangement and the fluorescent biogenic amines appear to be released in the interstitium by degranulating mastocytes, nearly entirely negative to fluorochromization.

Animals↗
PubMed1978

[Action of HCG on isolated and cultivated sow ovarian granulosa cells].

Granulosa cells harvested from cavitary follicles of varying diameters have been cultivated in vitro in basic conditions and in presence of HCG, and then tested for the activity of steroid-3 beta-ol-dehydrogenase and observed with a scanning electron microscope. As concerns the enzymatic activity, the cells of the smaller follicles (5--6 mm) show a very low reactivity which increases in presence of HCG. In the cells of the larger antral follicles (8--9 mm) the reactivity is remarkably higher, particularly when HCG is added to the medium. With the scanning electron microscope the appearance of the granulosa cells of the larger follicles cultivated with HCG is very peculiar because of the presence on the cell surface of many cytoplasmic evaginations. These are more numerous and thinner than those normally found on the uncultured cells and on the cells cultivated in basic conditions.

3-Hydroxysteroid Dehydrogenases↗