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Biomedical subjects

G C Lopes

Publications and source records attributed to G C Lopes.

5 recordsLinked to original sources

A 2-year evaluation of moisture on microtensile bond strength and nanoleakage.

OBJECTIVES: This study evaluated the effect of moisture on the resin-dentin mu-bond strength (BS) and silver nitrate uptake (SNU) of three adhesive systems (Single Bond, One-Step and Syntac Single Component) soon after bonding (IM) and after 2 years of water storage (2Y). METHODS: Dentin surfaces were bonded on a dry (D), moist (W) or over-wet surfaces (OW). After restorations were constructed, specimens were stored in water (37 degrees C/24h). Resin-dentin sticks were prepared (0.8mm(2)) and they were divided for immediate (IM) and 2-year storage (2Y) testing. Half of the specimens from each period of time were tested in tension at 0.5mm/min and the other half was immersed in silver nitrate and examined by SEM-EDX. The data was analyzed by three-way repeated measures ANOVA and Tukey's tests (alpha=0.05). RESULTS: The overall BS (MPa) in the IM group under W condition was higher than in D and OW groups. After 2Y, the BS in W was lower than in the IM group, however higher than in the D and OW for OS and SB. The overall silver nitrate deposition (%) in the IM group under D, W and OW were similar. In the 2Y groups, the nanoleakage was higher than IM groups, however the increase was less pronounced in the W condition. SIGNIFICANCE: Higher BS and a significantly lower nitrate uptake were observed for IM groups, for OS and SB. Under W conditions, the BS reduction over time was less pronounced and less nitrate uptake occurred.

Absorption↗

All-ceramic post core, and crown: technique and case report.

UNLABELLED: The functional and esthetic restoration of severely compromised anterior teeth is a challenge to clinicians in their daily practice. The duplication of the optical characteristics of an intact tooth, including shade, translucency, and fluorescence, is often made difficult by the use of metal infrastructures, such as post-and-cores and copings. The development of reinforced ceramics and non-metallic post systems made possible the generation of metal-free ceramic restorations in severely compromised anterior and posterior teeth. This article discusses one available technique for the generation of a single all-ceramic restoration involving the use of a zirconia- or leucite-reinforced post-and-core system and of an all-ceramic crown system. A representative clinical case is presented illustrating the potential of the technique. CLINICAL SIGNIFICANCE: Modern ceramic restorative systems offer the possibility to closely match the natural optical characteristics of lost tooth structure. Posts, cores, and copings fabricated in ceramic materials represent a promising alternative to metals in the restoration of severely compromised anterior teeth.

Adult↗

Effect of a sodium hypochlorite gel on dentin bonding.

UNLABELLED: It has been suggested that the hybrid layer (HL) does not play any important role in the mechanism of adhesion to dentin. To substantiate this hypothetical insignificance of the HL, sodium hypochlorite (NaOCl) has been used to remove collagen from etched dentin prior to bonding. OBJECTIVES: The present study was conducted to determine the effect of a commercial 10% NaOCl gel on the dentin shear bond strengths and HL ultra-morphology of two simplified dentin adhesives. The null hypothesis tested was that treatment of etched dentin collagen with NaOCl would not compromise dentin bonding. METHODS: The labial surface of eighty bovine incisors was polished to expose middle dentin. The specimens were randomly assigned to two total-etch adhesive systems (N = 40): Prime&Bond NT (Dentsply Caulk); and Single Bond (3M Dental Products Division). After rinsing off the etchant, one drop of 10% NaOCl (AD Gel, Kuraray Ltd.) was applied to the etched dentin surface and left for 0 (control), 15, 30, or 60 s. The gel was rinsed off with water and the dentin surface kept visibly moist prior to the application of the adhesive as per manufacturer's instructions. The respective composite resin was subsequently applied and light-cured. After 24 h in water at 37 degrees C, the specimens were thermocycled for 500 cycles in baths kept at 5 and 55 degrees C and the shear bond strengths measured. The data were analyzed with two-way ANOVA. For TEM, sixteen dentin disks were taken from middle dentin of extracted human third molars, assigned to the eight treatment sequences, and observed. RESULTS: The increase in the NaOCl application time resulted in a progressive decrease in shear bond strengths for both dentin adhesives. For Single Bond, the application of AD Gel for 60 s resulted in a reduction of bond strengths to 38% of that obtained for the control. For Prime&Bond NT, the mean bond strength obtained when AD Gel was applied for 60 s was 31% of that obtained for the control. The application of AD Gel resulted in distinct morphology for each one of the two adhesives tested. For Single Bond, the general morphology of the collagen network was maintained, regardless of the deproteinization time. The interfibrillar space within the collagen network increased with increasing deproteinization times. For Prime&Bond NT, the general appearance of the HL was maintained for deproteinization times of 15 and 30 s. When the NaOCl gel was applied for 60 s, the morphological appearance of the HL lost its fibrillar arrangement. While remnants of the collagen fibers were observed in one of the dentin disks, the other specimen showed an amorphous structure without any discernible HL morphological features. SIGNIFICANCE: The integrity of the collagen fibrils left exposed upon acid-etching plays a major role in the mechanism of adhesion of the specific adhesive systems tested in this study. The intermingling of the adhesive monomers with the filigree of collagen fibers or HL should still be considered the paramount dentin bonding mechanism.

Acid Etching, Dental↗

Effects of repeated use on bond strengths of one-bottle adhesives.

OBJECTIVE: Most one-bottle adhesives contain organic solvents (acetone or ethanol) that displace the water entrapped within the network of collagen fibers in etched dentin. Acetone and ethanol are volatile substances that could easily evaporate from bottles during use. The objective of this in vitro study was to assess the effects of repeated opening of bottles on dentin shear bond strengths of 4 adhesives. METHOD AND MATERIALS: Eighty freshly extracted bovine incisors were mounted, polished to 600 grit, and randomly assigned to 8 groups (n = 10). After the application of the materials to 40 specimens at baseline, adhesive use was simulated by opening bottles for 1 minute, 2 times a day, for 3 weeks, except during weekends, when the bottles were kept refrigerated. After 3 weeks of simulated use, the same adhesives were applied to 40 additional specimens. Resin composite was applied to dentin in a No. 5 gelatin capsule and was light-cured. After 500 thermal cycles (5 degrees C to 55 degrees C), shear bond strength testing was performed with a universal testing machine at 0.5 cm/min. RESULTS: Adhesives containing ethanol or water had similar mean bond strengths at baseline and at 3 weeks. The acetone-based material had a significantly lower mean bond strength at 3 weeks than at baseline. CONCLUSION: Acetone-based adhesives may have a shorter useful life than ethanol- and water-based adhesives.

Acetone↗

Bond strengths of new simplified dentin-enamel adhesives.

PURPOSE: To compare the in vitro shear bond strengths (SBS) of five simplified dentin adhesives. The tested hypothesis was that the recently introduced simplified adhesive systems would have similar or higher SBS than an existing simplified acetone-based adhesive used as a control. MATERIALS AND METHODS: 100 flat bonding sites were polished to 600-grit on the labial surface of bovine incisors mounted in acrylic resin. 50 teeth were ground to expose enamel, while the remaining 50 specimens were prepared to expose middle dentin. The specimens were randomly divided into five equal groups to be treated with simplified dentin adhesives: Dentastic Uno, EasyBond, Gluma One Bond, One Coat Bond, and One-Step (control). A composite post was bonded to each treatment area. After thermo-cycling, enamel and dentin shear bond strengths were determined using an Instron testing machine and the data were submitted to statistical analyses. RESULTS: Mean enamel bond strengths ranged from 14.6-28.4 MPa. One Coat Bond had the highest mean enamel SBS, but it was not significantly higher than those of Gluma One Bond and Dentastic Uno. EasyBond and One-Step had statistically similar mean enamel SBS and these were significantly lower than the mean enamel SBS of the other three adhesives. For dentin, mean SBS ranged from 14.8-21.7 MPa. Dentastic Uno had the highest mean dentin SBS, but it was not significantly greater than those of One Coat Bond and Gluma One Bond. Although One Step had the lowest mean dentin SBS, it was not significantly different from those of either EasyBond or Gluma One Bond.

Analysis of Variance↗