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Biomedical subjects

G Chacón

Publications and source records attributed to G Chacón.

5 recordsLinked to original sources

Journey length and high temperatures: effects on rabbit welfare and meat quality.

The transport of domestic animals by road can increase levels of stress and decrease meat quality, especially in unfavourable climates. The purpose of this study was to investigate the effect of journey duration and vertical position on the transport truck on some physiological indicators of stress and on instrumental meat quality parameters in commercial rabbits. In the summer months (June and July, 2003), 78 rabbits were subjected to either long (7 h) (LJ) or short (1 h) (SJ) journeys (3 replicates each, n = 6) between the farm and an abattoir in northern Spain. The position (top, middle, or bottom) occupied by the rabbits on the Multi-Floor cage Rolling Stand (MFRS) of the transport truck was recorded. Blood samples were collected at sticking and meat pH was measured at 24 h post-mortem (pH24). At 48 h post-mortem, samples of the M. longissimus dorsi were used to determine water-holding capacity (WHC) and instrumental tenderness using an INSTRON machine. The levels of corticosterone, glucose, lactate, and creatine kinase were slightly higher in LJ than in SJ samples, but the difference was not statistically significant (p < or = 0.10). Independent of journey length, rabbits in the middle and bottom of the MFRS showed higher levels (p < or = 0.05) of glucose and creatine kinase (middle), and corticosterone (bottom) than the rabbits located at the top floor. The pH 24 and WHC values of the SJ and LJ rabbits did not differ significantly. Journey length had a significant effect (p < or = 0.01) on meat tenderness. The meat from rabbits subjected to LJ had higher values of maximum stress and total toughness than did the SJ rabbits (p < or = 0.05). There were similar differences (p < or = 0.05) between LJ and SJ rabbits in their stress values of compression at 20% and 40% (measures of the tenderness of raw meat). In hot weather, the position on the transport truck appeared to have a greater effect on rabbit welfare than the duration of the journey.

Abattoirs↗

Scoring system for evaluating the stress to cattle of commercial loading and unloading.

The loading and unloading of cattle for road transport is stressful but the stress is difficult to evaluate in terms of the welfare of the cattle. Over one year, 40 normal commercial journeys in northern Spain were analysed in terms of time limits and behavioural events in order to design an objective method for assessing the stresses imposed on the animals, and a scoring method was developed for assessing the welfare of the animals during loading and unloading. Several definitions of time intervals were assessed to calculate a time score per animal, and easily observable behavioural events were scored and combined with the time score to obtain a total loading/unloading score. More than half of the loadings and unloadings involved turns, slips and vocalisations. Mounts and bouts of fighting were infrequent and balks and falls were significantly more frequent during loading than unloading. The plasma concentrations of cortisol, glucose and lactate, the activity of creatine kinase and the pH of the meat 24 hours after the animals were slaughtered were also measured. The results indicated that loading was more stressful than unloading and that higher scores implied significantly higher levels of stress.

Abattoirs↗

Effect of commercial transport in Spain on cattle welfare and meat quality.

The purpose of this work was to determine the effect of transport of cattle from the farm to the abattoir on the animal's welfare and post mortem meat quality (instrumental and sensorial). Thirty-nine commercial journeys were followed in Spain from July 2000 to July 2001 at varying distances from a local abattoir in the city of Zaragoza. Blood samples were taken at the farm and at sticking and meat pH24 was measured at 24 hours post-mortem. Samples from the M. longissimus dorsi thoracis were taken from 48 slaughter bulls and analysed in terms of instrumental and sensorial qualities. Cortisol, glucose, CPK, lactate and N:L ratio increased during transport compared to control values. Cortisol was higher after 1-2 h transport compared to journeys that were less than 1 h or more than 2 h long. In general the parameters indicated slightly more stressful conditions under winter conditions than in the summer. Meat tenderness measured by a Warner-Bratzler device demonstrated that there was no significant effect of either transport time or season. There was no significant change in pH24 or meat colour at 24 h with transport time. Transport time had a significant effect (p < 0.05) on overall liking and animals transported in summer had slightly more tender meat and higher overall liking.

Abattoirs↗

Tuberculous meningitis. Short course of chemotherapy.

In March 1986, we began a 6-month short course trial of therapy for tuberculous meningitis, in which 28 patients were analyzed. The diagnosis was based on the following cerebrospinal fluid test results: in 53.5% of the cases, Mycobacterium tuberculosis was identified by direct smear; in 57%, culture in Löwenstein-Jensen medium was positive; in 83.3%, the detection of anti-bacille Calmette-Guérin (BCG) antibodies by enzyme-linked immunosorbent assay was positive; and in 74%, the dosification of adenosine deaminase activity was positive. In addition, in 21.4% of the cases, the diagnosis was established by means of autopsy findings. Moreover, the diagnosis was supported by bacteriological analyses from another tissue or body fluids. Despite the administration of an antituberculous therapy, 32.4% of the patients died: all of the decreased had reached the last stage of the disease by the beginning of treatment. Sixteen percent of the patients who survived after more than 18 months of follow-up after therapy had ended suffered neurological sequelae. With the 6-month therapeutic regimen, the morbidity/mortality is similar to that found in the longer-course therapies. The latter regimen is therefore thought to be a good and acceptable therapeutic option for the treatment of tuberculous meningitis.

Adolescent↗

[The type-A behavior pattern and cardiovascular reactivity in executive managers].

With the purpose of studying the relationship between type A behaviour pattern (TABP) and cardiovascular reactivity in the natural work environment, the presence of type A, heart rate (HR) and systolic and diastolic blood pressure (SBP, DBP) during two cognitive tasks were evaluated in a group of business managers (46 men and six women) of a large organization. Habits associated with cardiovascular risk (sedentarism, smoking, alcohol consumption and obesity) as well as other biological risk factors (familiar and personal history of cardiovascular problems, cholesterol and triglyceride level) were also assessed. On results showed that when solving cognitive tasks--abstract reasoning (AR) and mental arithmetic (MA)--within the work environment, these stimuli produced a significant reactivity in heart rate, SBP and DBP and also in a subjective measure of stress. The majority of participants reached peak values of HR and SBP during the MA task, whereas DBP was mostly activated during the AR task. SBP was significantly higher at the beginning of the experimental session than during the resting periods or between tasks, and HR at the beginning of the session, was associated with the level of performance. Type A's and B's differed in their SBP taken just before leaving the session. These results indicate the importance of taking into account, aside from the resting measures, the entering and leaving periods of an experimental session. A very high proportion of the subjects studied were classified on type A behaviour (85%). Sedentarism, alcohol consumption and family history were also more frequent in this group. A very high proportion of participants had more than two biological risk factors or habits associated with cardiovascular risk. A's and B's were significantly different in personal history and hypertension, being A's at higher risk. The reduced number of type B's in this group did not allow further conclusions.

Adult↗