Biomedical subjects

G Christoff

Publications and source records attributed to G Christoff.

3 recordsLinked to original sources

PubMed1979

Growth of human acute myeloblastic leukemic (aml) cells in vitro.

Human normal and granulocytic leukemic progenitor cells produce colonies and clusters when cultured in the plasma clot system in vitro. The number of colonies formed and their cellular composition was comparable to that reported for the system (soft agar) usually used for these studies. The plasma clot system has the advantage of permitting in situ morphologic and cytochemical characterizaion of the cells within the colonies. A comparison was also made of the growth of leukemic cells in suspension cultures placed within conventional and modified Marbrook flasks. These studies demonstrated no advantage for the Marbrook system in cultures incubated for up to 7 days.

Culture Techniques↗

PubMed1978

Friend leukemia cells: relationship between differentiation, clonogenicity and malignancy.

Friend leukemia cells were cultured in vitro in the presence or absence of agents which induce erythroid differentiation. The cultures were harvested and the degree of differentiation determined. Clonogenicity of the cells in vitro and malignancy in vivo were determined as well. There was an inverse exponential relationship between the degree of differentiation and the clonogenicity of the culture. Differentiation was also associated with a modest decline in malignancy. Of interest was the observation that bromodeoxyuridine inhibited the biochemical manifestations of erythroid differentiation, but did not prevent the decline in clonogenicity which accompanied differentiation.

Animals↗

PubMed1976

Cryoprotective agents as inducers of erythroleukemic cell differentiation in vitro.

The ability of families of compounds with known and potential cryoprotective properties to induce the differentiation of Friend leukemia cells in vitro was studied. For each agent, both the proportion of differentiated cells in the culture and the total amount of heme/10(7) cells were determined. Within each family of compounds there was a direct correlation between a compound's cryoprotective ability, its ability to donate electron pairs for hydrogen bonding (basicity), and its ability to induce differentiation. While individual agents differed with respect to the proportion of cells which were induced to differentiate, the biology of the process of differentiation appeared to be similar, regardless of the agent used. A cell line which was unresponsive to DMSO was responsive to other inducers, suggesting that this DMSO-resistant cell line differed from its parent DMSO-responsive cell line either in its metabolism of the inducers or in the ability of the inducers to enter the cell. Alternatively, there may be more than one mechanism involved in the chemical induction of differentiation.

Acetamides↗