[Studies of the antibiotic action of a strain of Staphylococcus on Mycobacteria].
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Mycobacterium↗
Biomedical subjects
G Coman
Publications and source records attributed to G Coman.
[Human infection with Campylobacter jejuni/coli].
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Animals↗
[Preliminary considerations in a semiquantitative microbiological study in catheter-related infection].
In the interval September 1993-April 1994, 38 catheters from children admitted to the Children's Hospital of Iaşi were examined microscopically and by semiquantitative culture, in the children with sepsis and/or positive catheters hemocultures being performed concurrently. Twenty eight of these catheters proved to be sterile, the other 10 being positive to coagulase-negative staphylococci, gram-negative bacilli from Klebsiella-Enterobacter group, Candida species, the number of colonies/plate varying generally in concordance with the microscopic positive score. Of the 6 cases with > 50 colonies/plate, 3 progressed to septicemia, their hemocultures being positive to the same bacterial species demonstrating an identical sensitivity spectrum to antibiotics (2 cases with Enterobacter cloacae, 1 case with Klebsiella oxytoca). These results correlated with a bacterioscopic score > +1, in a case, which concomitantly presented local phenomena of phlebitis, a PNC score +3 being recorded. To conclude with, the results of microscopic examinations and semiquantitative culture can differentiate the contaminated catheters from those at bacteremic risk. For defining the cutoff level of the significant number of colonies/plate, more positive samples are needed.
Adolescent↗
Neurosurgical stress and immune function.
The present study was carried out in 31 neurosurgical patients with unaffected consciousness and concerned by the severity of their condition. The severity of stress was established by the comparison between the pre- and postoperative peripheral blood lymphocyte populations and subpopulations (B, T, Th lymphocytes, serum complement and circulating immune complexes). The peripheral blood lymphocyte populations and subpopulations were determined by the technique of sheep red blood cell rosette. Circulating immune complexes were determined by precipitation of polyethylene glycol (PEG, M = 6000) whereas the serum complement by the usual 50% hemolytic method. Our data show that the decrease in Th cells and the consecutive diminution of antibody production is a cause of lower resistance against cancer and infections.
Adult↗
[Action in vitro of associations of tuberculostatic drugs on strains of mycobacteria with multiple drug resistance].
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Antitubercular Agents↗
Tumoral infiltrate after local treatment with interferon in squamous cell carcinoma.
Using monoclonal antibodies UCHL-1 (T lymphocytes), MT-1 (pan T) and L-26 (B lymphocytes) in the study of the tumoral infiltrate after local treatment with alpha interferon (Roferon) in patients with squamous cell carcinoma of the lower lip, it was observed that: the proportion of UCHL-1 positive cells was between 30% and 80%, the proportion of MT-1 positive cells was of 85% and that of the L-26 positive cells was of 30% of all the cells in the infiltrate. In the area in which after treatment with interferon the tumoral structures had disappeared, the proportion of T lymphocytes was smaller than in the areas in which the tumoral structures were still present. The therapeutic effect of interferon is due both to the direct effects on the tumoral cell and also to the indirect effects, namely the activation of the cytotoxic T lymphocytes and of other cells in the tumoral infiltrate.
Adult↗
[The measurement of plasma viral loads in HIV-infected children. Their possible role in parenteral transmission].
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AIDS-Related Complex↗
[Action of some antiseptic substances on the incidence of post-extraction bacteremia].
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Anti-Infective Agents, Local↗
[The isolation of Arcanobacterium haemolyticum from the pharyngeal exudate of children].
A. haemolyticum is involved in acute pharingotonsillitis in present, reaching a maximal incidence between 10 and 30 years of age. The authors examined 7402 throats swabs obtained from children between March 1995 and February 1996. The swabs were plated on a selective culture medium: 5% sheep blood agar added with 3.5% NaCl; the plates were incubated for 48 h in aerobic conditions. The colonies of A. haemolyticum have been suspected on the basis of a) the growing time interval (48 h); reduced size (diameter less than 1 mm); c) the presence of a narrow zone of incomplete beta-haemolysis and d) the typical pitting of the culture medium beneath the colony. The definitive identification was realised on the basis of the microscopical appearance (diphtheroid gram-positive bacilli), negative catalase test and inverse CAMP test (narrowing of the haemolytic zone produced by staphylococcal beta-lysine). The frequency of A. haemolyticum in the throat swabs investigated was 0.07% (5 cases), comparing with S. pyogenes which was found in 8.21% of cases.
Actinomycetaceae↗
Antibiotic resistance study of invasive S.pneumoniae.
121 invasive pneumococci isolated from meningitis, septicemia, pneumopathies and other clinical infections (osteo-arthritis, peritonitis, sinusitis, otilis, conjunctivitis, plagues) were screened for susceptibility to penicillin G (P), erythromycim (E), chloramphenicol (Cl), tetracycline (T), ceftriaxone (Cro), and sulfametoxazole trimethoprim (SxT) by diffusimetric method, by MICs to P and Cro by agar dilutions and E-test. 48.8% were penicillin resistant (MICs > 0.12 microgram/ml), 22.7% being highly resistant (MICs 3-8 micrograms/ml). Any strain was resistant to P only, but patterns with P included were frequent (55.8%). Resistance to SxT was highest (monoresistance 25.6% as well as polyresistance 69.8%). 72.9% were resistant strains to > or = 1 antibiotic with 12 patterns of resistance (1-5 antibiotics). All of the strains were susceptible to Cro (MICs-0.003-0.5 microgram/ml). Resistance was closely correlated to serotypes 6,9,14,19,23 to site of isolation and diagnosis. Pneumococci from meningitis were 2-3 times more susceptible to P,E,Cl,T and SxT than the isolates from pneumopathies or other infections.
Ceftriaxone↗
[Pyogenic infections with different locations caused by Streptococcus anginosus alone or in association with anaerobic bacteria].
Isolation and identification of S. anginosus from pathologic products are possible to be realised in informed clinical laboratory, allowing so a better knowledge of these infections' incidences and on adequate treatment. The authors' report 11 cases of pyogenic infection caused by S. anginosus. Five out of these 11 cases evaluated as mixed infections, S. anginosus being associated with anaerobic bacteria. 10 children hospitalised in surgery Department of Children Hospital had infections with different localisations; necrotizing fasciitis, preknee cap abscess, generalised peritonitis, abscess postappendectomy, pleurisy and acute mediastinitis, knee arthritis, acute osteomyelitis of mandible and an infection of the fracture's focus in upper 1/3 of the thigh bone. For all these patients the favoring factor was represented by a traumatic or surgical lesion of the skin or diverse mucosa; oral, oesophageal, intestinal, allowing the access of the normal flora of these covers to normally sterile sites. The eleventh case was an adult with a lung abscess and pleurisy, as a complication of an aspiration pneumonia. The treatment of S. anginosus infections consisted especially in penicillin or ampicillin, associated with metronidazol when anaerobic bacteria were present.
Adolescent↗
[HIV infection in a seronegative child diagnosed by isolation of the virus. The significant immunological and epidemiological signs].
HIV-1, subtype F was isolated from a seronegative child aged 2.5 yr. ELISA tests (Behring HIV-1 + 2, Abbott HIV-1 + 2, Wellcozyme HIV-1 Recombinant, Clonatec HIV-1 + 2, Genelavia Mixt), and also rapid tests (Abbott Pack, Serodia) were all negative, although some of them presented borderline reactivities. Western Blot (Cambridge Biotech) revealed an undetermined profile (traces of anti-gp160 plus anti-p24). A new WB test (Sanofi Dg. Pasteur) performed at a higher serum concentration (1/25) revealed a complete antibody profile, despite the very low intensity of bands. A new serum sample prelevated 6 month later was completely negative on all tests used. Both samples, were negatives in WB for HIV2 and HTLVs. A heparinised blood sample was used for the co-cultivation of PBMC and was proven to be positive in the 14th day of culture. The isolated DNA from end-culture cells was subjected to PCR amplifications for Heteroduplex Mobility Assay direct subtyping (primers ES7 and ES8) and for the investigation of genotypic sensitivity to AZT (primers A/NE1). Lymphocyte populations phenotyping revealed leukocytosis (> 15,000/mL) with a predominance of the CD8+ subset CD4/CD8 ratio was < 1. Plasmatic HIV-1 load (measured by bDNA--Chiron) did not reached detectable levels of HIV-1 RNA. p24 Ag assay (EIA-Coulter) revealed a detectable p24 antigenemia only in the first serum sample and only after acid dissociation. So, this patient may present an integrated HIV-1 infection until now "silent".
AIDS Serodiagnosis↗
[Helicobacter heilmanii gastritis: the bacteriological, endoscopic and histopathological considerations in 18 cases of infection in children].
The investigations on the presence of Helicobacter pylori in gastric mucosa biopsy specimens have evidenced another morphologically distinct spiral bacterium, Helicobacter heilmanii, which is associated with histopathological lesions of antral gastritis. The authors found 18 cases of such infection in a number of 1508 children with dyspeptic manifestations examined endoscopically. While H. pylori-associated infection was detected in 48.7% of the cases, H. heilmanii-associated gastritis had a much lower prevalence, 1.1%. The diagnosis was made by microscopic examination of the gram fuchsin-stained smear from a biopsy fragment, the urease test being more commonly erratic or late positive. In 10 cases, the endoscopic examination of gastric mucosa revealed nodularity of antral mucosa, in one of these cases a ulcer lesion at the level of the great curvature being also associated. Histopathological changers of active chronic gastritis have been found but in 4 out of children, the examined fragments of gastric mucosa being collected in most cases from the fundic area: in the child with gastric ulceration the histopathological lesions were of antral acute gastritis. Clinical, bacteriological, endoscopic and histological cure of H. heilmanii gastritis is possible by therapy with bismuth compounds alone.
Adolescent↗
[The isolation and subtyping of the human immunodeficiency virus (HIV) from children in Moldova. The evidence for subtype F].
HIV epidemic related to nosocomial transmission in Romanian nursed children represents up to now more than 50% of paediatric AIDS cases in Europe. Although the sources of this epidemic are obscure, HIV-1 subtype F, a minor form in Brasil and Africa, realised a founder effect in this risk group, thus becoming the major form in Romanian epidemic. This study presents the results of the virus isolation from HIV-infected children in Northeast Romania. Heteroduplex mobility assay (HMA) was performed in order to establish HIV-1 viral subtype for 6 isolates. All tested HIV-1 strains were proved to belong to F subtype. So, our study confirm that HIV-1 subtype F is responsible for the epidemic in the risk group of Romanian nosocomially infected children.
Acquired Immunodeficiency Syndrome↗
[The frequency of Streptococcus anginosus in the pharyngeal exudate from children and its differentiation from other beta-hemolytic streptococci].
UNLABELLED: S. anginosus is a commensal of the oro-pharyngeal mucous membrane without any signification for the local pathology. Ignoring the existence of beta-haemolytic colonies of this species, the risk to report the presence of some beta-haemolytic streptococci that actually belong to the normal flora exists. The antigenic identifications of beta-haemolytic streptococci maintain the confusion either, S. anginosus being able to react with specific sera anti group G, C or A. In our study, two identification criteria out of those available demonstrated a high value: the small or very small colonies' size, especially in secondary cultures and the production of acetoin (Voges-Proskauer test). S. anginosus was isolated with a quite great frequency in pharyngeal exudate from children: 41 strains out of 90 strains of beta-haemolytic non-group A streptococci were S. anginosus. Antigenically they belonged, in numerical order to groups C, G, F or they were ungroupable. IN CONCLUSION: The microbiologist has to identify, but not to report the presence of S. anginosus in pharyngeal exudate, it being a normal component of the oro-pharyngeal flora. Doing so, a better evaluation of the clinical signification of the other beta haemolytic streptococci's non group A will be possible.
Antigens, Bacterial↗
Serological level of ICAM and ELAM adhesion molecules in allergic vascularitis.
A 24-patient lot with hypersensitivity vasculitis was investigated for serological determinations of ICAM and ELAM adhesion molecules. Determinations were made in attack and in remission. Over two thirds of the cases presented elevated serological levels of ICAM and ELAM in attack, with twofold higher values than normal. In remission, in the absence of clinical signs, ICAM and ELAM values were normal in 19 cases (ICAM) and 22 cases (ELAM). Serological level of ICAM and ELAM was concordant with serological level of IL-2, IL-6, circulating immune complexes and clinical status. The increased values of ICAM and ELAM are due to the expression of these molecules both on the surface of endothelial cells and on immune cells. The adherence of leukocytes on the endothelial cells, by adhesion molecules involvement, followed by their extravasation represents an important event in the vascular lesion pathogeny of the hypersensitivity vasculitis.
Adult↗
[Corynebacterium urealyticum in urinary infections in children].
The urinary infection caused by C. urealyticum is a rare circumstance, especially in children. Authors present the etiology of these infections, in children admitted in the Pediatric Clinical Hospital "Sf. Maria", Iaşi, during 1997. This recently recognised uropathogen was isolated from three cases. Compared to other etiological agents, the frequency was 0.75%. This study presents the diagnosis and identification criteria for C. urealyticum and therapeutic challenges of this infection.
Child↗
Photodynamic treatment of basal cell carcinoma and squamous cell carcinoma with hypericin.
Hypericin displays antiproliferative and cytotoxic effects on tumor cells. This effect depends on photodynamic activation with visible light and oxygen. Hence, we explored its potential use in treating skin cancer. Eight patients with squamous cell carcinoma (SCC) and eleven patients with basal cell carcinoma (BCC) were treated topically with hypericin. After intralesional injection, the hypericin was irradiated with visible light. Patients with SCC were given 40-100 micrograms hypericin intralesionally, 3-5 times per week for 2-4 weeks; patients with BCC 40-200 micrograms hypericin 3-5 times per week for 2-6 weeks. Hypericin displayed selective tumor-targeting: penetration in the surrounding tissues did not induce necrosis or cell loss and even the generation of a new epithelium at the surface of the malignancy was noticed. The effectiveness of the therapy depends on the concentration and total dose of hypericin, the frequency and duration of the therapy; clinical remissions can be expected after 6-8 weeks.
Adult↗