PubMed Health⌕ Search

Biomedical subjects

G D Kaminskiĭ

Publications and source records attributed to G D Kaminskiĭ.

At least 19 recordsLinked to original sources
PubMed1995

[Validity of epidemiological classification: construction of a discriminant criterion].

A new criterion has been proposed to assess the validity of group division differences obtained by preliminary cluster analysis. The method was used to provide evidence for the validity of differences between the groups as to epidemiological classifications. The paper shows it expedient to use the method in the epidemiological analysis.

Cluster Analysis↗
PubMed1989

[Dissociation and ultrastructure of the cells of Shigella sonnei isolated from gnotobiotes and obtained in vitro].

The dissociation variants of S. sonnei in phase I, isolated from germ-free rats and obtained in vitro, have been studied. Such dissociation variants have been found to form colonies with classical and atypical morphology. The electronmicroscopic study has revealed that different dissociation variants include small dense cells with the markedly thickened cell wall and pronounced microcapsule and spheroplasts with the damaged cell wall and less pronounced microcapsule. The formation of these cells is supposed to be the way of the adaptation of S. sonnei in the course of the infectious process and linked with changes in their virulence in the population cycle.

Animals↗
PubMed1989

[The plasmid heterogeneity of Shigella sonnei phase-I and -II strains].

The plasmid composition of S. sonnei standard strains has been studied by the method of electron microscopy of the preparations of plasmid DNA. In S. sonnei cells I-941-HP, phase I, plasmids of 2,500; 5,000; 5,600; 6,100 and 6,800 base pairs, as well as plasmids of 85,000-117,000 and 170,000-235,000 base pairs have been detected. In S. sonnei cells, phase II, plasmids of 2,500; 4,900 and 6,100 base pairs, as well as plasmids of 85,000-109,000 base pairs, have been found. Thus, virulent S. sonnei in phase I contain additional plasmids of 5,600; 6,800; 110,000-117,000 and 170,000-237,000 base pairs. The range of plasmid lengths between 85,000-117,000 and 170,000-237,000 base pairs exceeds the usual background of electron-microscopic studies, which makes it possible to come to the conclusion on the intrastrain heterogeneity of these classes of plasmids. The suggestion has been made that the transition of S. sonnei from phase I to phase II is linked with the loss of fragments of the genetic material, limited by inverted DNA repetitions.

Base Composition↗
PubMed1989

[Fast and slow genome rearrangements: molecular and genetic mechanisms of changes in virulence].

Different mechanisms of virulence changes at the molecular and populational levels are discussed. The methodology and mechanisms of Streptococcus pyogenes variability are analysed. The views of the adaptive role played by insertion sequences are developed, and the design of an epidemiologico-genetic experiment is proposed, which consists in obtaining highly virulent insertion mutants and testing their properties.

Enterobacteriaceae↗
PubMed1986

[Phenomenology and molecular mechanisms of Shigella sonnei dissociation: its variability in model in vivo and in vitro systems].

The dynamics of the accumulation of phase II S. sonnei in the infectious process in germ-free rats, during cultivation and in subcultures was studied. The in vivo variability of S. sonnei showed a sharply defined phasic character of the process with the periods of the apparent absence of phase II, the increase of its occurrence and stabilization with the preservation of S-forms in minor amounts. Kanamycin-resistant phase II accumulated in vivo more rapidly than in vitro. Cultivation with sodium desoxycholate was found to accelerate the process of disassociation. The characteristic feature of all dissociants detected in all systems was their plasmid profile: in phase I, plasmids of 120 and 60 Md, as well as small plasmids, were detected; in phase II disassociants, plasmid with a molecular weight of 120 Md was absent. The restriction analysis of total plasmid DNA by means of restrictase EcoR1 showed the absence of 3 fragments in phase II while other 13 fragments were retained. These results open prospects for the molecular cloning of the gene antigen of phase II.

Animals↗
PubMed1986

[Controllable infections and the self-regulation of parasitic systems].

The control of the epidemic process is based on the knowledge of the mechanisms of self-regulation, developed in the process of evolution, in the relationship of the host and parasite populations under the changing conditions of the social and natural environment. The problem of the global and regional liquidation of infections controllable by means of immunoprophylactic measures is considered.

Animals↗