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Biomedical subjects

G Delespesse

Publications and source records attributed to G Delespesse.

At least 19 recordsLinked to original sources
PubMed1979

Distribution of beta-adrenergic receptors on human lymphocyte subpopulations.

A technique is described allowing the quantification and the characterization of specific beta-adrenergic receptors in intact living human lymphocytes. 125I-Iodohydroxybenzylpindolol, a potent beta-adrenergic antagonist was used to label specific binding sites on unfractionated lymphoid cells and on purified subpopulations of T (F1 and F2) and B cells. F1 and F2 were obtained by filtration through nylon wool column as previously described (Delespesse et al., 1976), they differ in their response to mitogens, and in their interactions with adherent cells and B cells. 125I-HYP binding to unfractionated lymphocytes was a saturable, stereospecific and rapid process with a dissociation constant of 2.5 10(-10) M and a binding capacity of 400--600 sites/cell. Bindings on unfractionated lymphocytes, purified B cells and T cells of the F2 fraction were similar. No detectable binding was noted on T cells from the F1 fraction. Enriched T cells obtained by a rosetting technique displayed 200 receptors/cell.

Adrenergic beta-Agonists↗
PubMed1979

Circulating immune complexes in various thyroid diseases.

In a study of 171 patients with various thyroid diseases, circulating immune complexes (CIC), measured by a C1q solid phase radioassay, were detected in 26% of the patients as compared to 8% of the control subjects. CIC were found in 33--55% of the patients with a well defined thyroid autoimmune disorder (Hashimoto's goitre, asymptomatic thyroiditis, spontaneous myxoedema and Graves' disease) and also in the same proportion of patients with diffuse goitre. CIC were correlated to the presence of serum antibodies to microsomal thyroid antigen but not to their titre. No relationship was observed between CIC and the age or sex of the patients and the presence of exophthalmos, or between CIC and the different thyroid function tests or serum anti-thyroglobulin antibodies. CIC were found in untreated patients as well as in those treated with prednisone, methimazole or thyroxine.

Adolescent↗
PubMed1979

[Incidence of antispermatozoidal antibodies in the serum of fertile women (author's transl)].

The authors describe a simple and reproducible-microagglutination technique for the detection of antispermatozoa antibodies. A positive reaction is detected in 2 p. 100 of women with a normal fertility (12/516), in 10 p. 100 of pregnant women (4/40) and in 32.5 p. 100 of patients with recurrent abortions. There is no relation between anti-HLA antibodies and the sperm agglutinating activity of a serum.

Abortion, Habitual↗
PubMed1978

Influence of ageing on IgE-mediated reactions in allergic patients.

The influence of age and sex has been evaluated in 326 allergic patients on the following parameters: total serum IgE concentration, serum concentration of specific IgE antibodies against seventeen common allergens, mean number of positive allergens, absolute numbers of circulating eosinophils, corticosteroid dependence and response to sodium cromoglycate (DSCG). The results indicate that age and sex significantly influence the IgE antibody production in these patients. The data strongly suggests the appearance during ageing of a progressive natural desensitization. This contrasts with the increased frequency of corticosteroid-dependent cases during ageing. There is an inverse relation between age and response to DSCG therapy.

Adolescent↗
PubMed1978

Methylmethacrylate hypersensitivity in orthopaedic surgery.

The sensitivity to methylmethacrylate monomer in 25 patients undergoing orthopaedic surgery was studied. No relation could be found between the cardiovascular reactions observed during the cementation of the femoral prosthesis and the complement system, investigated by measuring the serum concentration of the haemolytic complement, components 3 and 4.

Aged↗
PubMed1977

Treatment of chronic urticaria with a beta2-adrenergic stimulant.

Twenty-four patients with chronic utricaria resistant to conventional therapy were treated orally with terbutaline, a specific beta2 stimulant. This therapy was much more effective than antihistamines. At the dose of 1-25 mg three times a day, there were few side effects. The efficacy was independent of the IgE levels; it seemed greater in idiopathic cases than in cholinergic or pressure induced urticaria.

Adolescent↗
PubMed1977

IgE mediated hypersensitivity in ageing.

The serum IgE concentration is significantly lower in aged as compared with young people. This is more pronounced in males than in females (P less than 0-05). In fifteen aged (greater than 70 years) compared with fifteen young subjects (less than 40) the specific IgE antibody response to an injection of diphtheria toxoid was significantly reduced (P less than 0-01). The minimum concentration of anti-IgE antibody able to induce a weal and flare reaction when injected intradermally, was comparable in the two age groups. However, the diameter of the weal reaction was significantly reduced in the aged. It is concluded that the reduced incidence of atopic symptoms in old people might be secondary to an anomaly of both the immune system and the mast cells.

Adult↗
PubMed1977

Influence of levamisole on the immune response of old people.

Fifteen healthy people, over 70 years old, have been treated during 1 month with Levamisole at the dose of 150 mg a day, twice a week. They have been compared to 15 controls matched for age and sex. At the start of the study, each subject was injected with 0.2 ml of a diphtheria-tetanus vaccine. Levamisole had no effect on the following immune parameters known to be altered in old age: serum IgE concentration, specific IgE and IgG antibody response to diphtheria toxin, lymphocyte response to phytohemagglutinin (PHA). There was a slight and transitory effect of Levamisole on the isohemagglutinin titer. In vitro, Levamisole was weakly mitogenic for the peripheral blood lymphocytes from old and young control subjects but there was no influence on the response to PHA.

Aged↗
PubMed1976

In vitro response of subpopulations of human tonsil lymphocytes. I. Cellular collaboration in the proliferative response to PHA and Con A.

Human tonsil lymphocytes have been separated into three subpopulations of cells: purified B cells and two subsets of purified T cells (F1 and F2). B cells were obtained by rosetting with neuraminidase treated SRBC. F1 and F2 were separated by filtration on a nylon wool column using different speeds of elution. Purified B cells contained less than 5% T cells, the T cells preparations contained less than 5% B cells for F1 and 10 to 15% for F2, respectively. A significant contamination in cells not identified by any B or T marker was observed in purified B cells and in F1. Adherent cells enhanced the response of each lymphochte population to PHA and Con A. This explained the paradoxically low responsiveness of the purified T cells. Purified B cells did not respond to these mitogens in different culture conditions. However, a small B cell response was observed when they were cultured in the presence of mitomycin-treated T cells. Striking was the enhancing effect of B cells on the T cell response to PHA and Con A. This enhancing effect was observed even when B cells were treated with mitomycin or depleted in adherent cells. The comparison of the F1 and F2 response suggested that they contained distinct types of T cells.

B-Lymphocytes↗