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Biomedical subjects

G Di Francesco

Publications and source records attributed to G Di Francesco.

At least 19 recordsLinked to original sources

Neosporosis in water buffalo (Bubalus bubalis) in Southern Italy.

A study was carried on 1377 water buffalo serum samples from 50 farms in southern Italy to test the presence of Neospora caninum antibodies by indirect fluorescence antibody test (IFAT). Rabbit anti-buffalo immunoglobulins conjugated to fluorescein were used in the test. Fluorescence in sera dilutions above 1:200 was considered as indicative of the presence of N. caninum antibodies. The overall prevalence of infection in the animals was 34.6%. The prevalence increased in relation to the age of subjects and most of the herds examined (82%) were found infected. In two farms abortions and neurological signs were reported. No suppurative inflammatory lesions were seen, but few protozoan-like cysts were observed on foetal tissues by histology.

Abortion, Veterinary↗

Detection of Mycoplasma mycoides subspecies mycoides in tissues from an outbreak of contagious bovine pleuropneumonia by culture, immunohistochemistry and polymerase chain reaction.

Postmortem observations of 37 cattle from an outbreak of contagious bovine pleuropneumonia (CBPP) in north Italy in 1993 were made at the abattoir, where samples of lung and tracheobronchial lymph node tissues were taken for culture and identification of Mycoplasma mycoides subspecies mycoides (MmmSC), immunohistochemistry with the peroxidase anti-peroxidase (PAP) system, and molecular detection by the polymerase chain reaction (PCR) amplification of specific DNA from MmmSC. Nasal swabs were also taken for testing by PCR Lung pathology typical of CBPP was observed in 38 per cent of the animals, and MmmSC was isolated from 19 per cent DNA of MmmSC was detected by PCR in 64 per cent of lung samples and 35 per cent of the nasal swabs. Staining of lung tissue and lymph node tissue by PAP was positive in 27 per cent and 30 per cent of cases, respectively, and was a useful back-up test. These results suggest that PCR amplification from lung tissue may be used as a rapid and accurate confirmatory test for cases with pathology resembling CBPP.

Animals↗

[Psychological adaptation to hysterectomy. Experience with 40 patients].

To assess how hysterectomy was psychologically overcome we refer to the results obtained from a questionnaire given to 40 patients. The questionnaire was divided into 3 parts: one statistic-epidemiologic, one to evaluate the patients' level of information, and a third aimed at studying post-operative psychological and sexual adaptability.

Adaptation, Psychological↗

Patterns of activity of perioral facial muscles during mastication in man.

The activity of buccinator, orbicularis oris superior and inferior, quadratus and triangularis muscles was recorded by surface and needle electrodes during spontaneous unilateral mastication of small or large boluses. Their activity was compared with that of the masticatory muscles masseter and digastric, and with the vertical movement of the jaw. The facial muscles were active during mastication, displaying both tonic and phasic activity. Although the cyclic activity was broadly linked to lowering of the jaw, there was no strict time correspondence between the activity of the various facial muscles themselves, or between the activity of the facial muscles and the digastric. The timing and amplitude of facial muscle activity were affected by the duration of the masticatory cycle, the side on which chewing took place, the size of the bolus, and whether or not lip to lip contact was made.

Adult↗

Inhibition of jaw-closing muscle activity by tactile air-jet stimulation of peri- and intra-oral sites in man.

There were two distinct periods of suppression in electromyograms of masseter and temporalis muscles in 8 subjects after light tactile stimulation (a puff of air) of the peri-oral zone; the latencies were 16 and 60 ms, respectively. These effects were similar to those induced by painful electrical stimulation. A single period of suppression with a latency of 50 ms, was evoked by tactile stimulation of the mucosa of the hard palate or of the tongue. Threshold for suppression was lower in mucosa than skin. Low-threshold and fast-adapting receptors are likely to be involved in this reflex inhibition.

Adult↗