DMSO-enhanced whole cell yeast transformation.
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Biomedical subjects
Publications and source records attributed to G Donald.
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Spiral fractures of the distal third of the tibial shaft can have problems of malunion or non-union that may be related to inability to assess the quality of the initial reduction of the fracture. We created typical two-fragment torsional tibial fractures in cadaver bones. The fracture fragments were mounted in a specially constructed jig that allowed simulation of various malreduction positions including varus and valgus angulation, antecurvatum, recurvatum, malrotation, and shortening. Anteroposterior and lateral radiographs and computed tomographic scans of the specimens were made. Measurements of the fracture gap were made at defined distances along the fractures, directly on the specimens as well as on the radiographs and computed tomographic scans. Excellent correlation was obtained between measurements of the gap on the specimens and on the computed tomographic scans, but plain radiographic measurements in thirty of thirty-four instances underestimated the true width of the gap. When shortening was introduced larger maximum gaps (of as much as eighteen millimeters) as well as greater discrepancies between measurements on the scans and plain radiographs were seen. The sizes of the fracture gaps were also greater for a given degree of shortening when the pitch of the spiral fracture was greater.
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Intramedullary nailing of the tibia is a controversial procedure for fractures of the tibial shaft. Experience with 50 fractures (58% acute, 8% pathologic, and 34% unsuccessfully treated previously by other methods) with an average follow-up period of one year proved that tibial Küntscher nailing is effective. Mean time to clinical union, with patients independently ambulatory and resuming all work activity, was 29 weeks. Union was demonstrated radiographically at 13 weeks. The major complications were delayed union (2 patients), nail protrusion at the knee (2 patients), osteitis (1 patient), and thrombophlebitis (1 patient). Antirotation wires were used in addition to a Küntscher nail for fractures with inherent rotational instability. Blocking screws were used for fractures predisposed to bending. Technical problems arose most often at the nail entrance site, in negotiation of Herzog's curve, and in selection of proper nail length and caliber. With experience and technical skill, even highly complex tibial fractures can be successfully treated by this method.
This report describes the clinical course of a 16-year-old Canadian-born Chinese boy who has progressive rubella panencephalitis. The progression, the lack of response to 'Isoprenosine', and the additional finding of myopathy are discussed. The clinical and pathological features of this rare, progressive neurological disorder are also summarized. In view of the last major rubella pandemic occuring in the mid-60s, it is likely that during the next few years physicians will diagnose an increased number of patients with this distinct neurological entity.
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