PubMed Health⌕ Search

Biomedical subjects

G Drewa

Publications and source records attributed to G Drewa.

32 records · Page 2Linked to original sources
PubMed1977

The effect of trasylol (Trascolan "Polfa") and p-aminomethylbenzoic acid (Pamba "Germed") on the experimental metastases of transplantable melanotic melanoma in golden hamster (Mesocricetus auratus, Waterhouse)

The influence of two proteinase inhibitors -- Trasylol (Trascolan "Polfa") and PAMBA ("Germed") -- on experimental metastases of melanotic melanoma has been studied in golden hamsters. Animals were injected intravenously with tumour cells (5x10(5) or 10(6) in 0.2 ml of saline). They obtained protease inhibitor intraperitoneally one hour prior, or one hour after melanoma cells injection. One group of hamsters were also inoculated with tumour cells preincubated 10 min. in saline containing Trasylol or PAMBA. A diminished number of experimental metastases to the lungs in each group has been observed.

4-Aminobenzoic Acid↗
PubMedDate not supplied

Enzymatic efficiency of erythrocyte antioxidant barrier and lipid peroxidation in children from families with high risk of early atherosclerosis.

The most important risk factors contributing to the development of atherosclerosis include lipid disorders and the predisposition to early ischaemic heart disease in the family. Atherosclerotic process proceeds with age and it develops as a result of oxide LDL modification at the level of vascular wall. Oxygen-free radicals take part in this process, which may probably be opposed by the antioxidant system of the body. The aim of this study was to compare the intensity of lipid peroxidation and the activity of antioxidant enzymes in children from the families with the risk of early atherosclerosis and in children without such predisposition. The activity of katalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined and the concentration of malonic dialdehyde--a lipid peroxidation marker was established. The study was conducted on 76 children aged 4-17 years, mean age 12 +/- 0.6 years. The risk group consisted of 56 patients with the history of hypercholesterolaemia and early atherosclerosis in the members of their families up to 45 years of age. Control group was formed of 20 subjects without such history. MDA concentration as well as the activity of antioxidant enzymes were determined with the use of adequate methods of spectrophotometry. The results obtained were subject to statistical analysis. The activity of antioxidant enzymes displayed considerable fluctuations in both groups of children, but these differences remained statistically insignificant in all the cases. Higher MDA concentrations in serum and in erythrocytes were observed in the risk group. These differences proved statistically significant (alpha < 0.05). On the basis of the present study and the analysis performed, it was found that the activity of antioxidant enzymes (CAT, SOD, GSH-Px) cannot serve as a parameter differentiating between children from the families with the risk of early atherosclerosis and children without such predisposition. Children with positive family history of hypercholesterolaemia and early atherosclerosis may demonstrate intensive lipid peroxidation, but this hypothesis requires further investigations.

Adolescent↗
PubMedDate not supplied

Antioxidant defense in children with chronic viral hepatitis B and C.

INTRODUCTION: The aim of the study was estimation of antioxidant defence state in children with chronic viral hepatitis. MATERIAL AND METHODS: We examined 100 children with chronic viral hepatitis who have serological and molecular markers of HBV or HCV infection. In all patients catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in erythrocytes were assayed. RESULTS: We observed statistically significant decrease of CAT and SOD activities and increase of GSH-Px activity in children with chronic hepatitis B and C. CONCLUSIONS: Our data suggest the possibility of insufficiency of antioxidant barrier in children with chronic viral hepatitis.

Adolescent↗
PubMedDate not supplied

Mitochondrial diseases.

Prevalence of mitochondrial diseases equals 1:10,000 of life-born infants. Mutations of mitochondrial DNA are their most frequent cause. The study presents short description of some of these diseases. The diseases often result in encephalomyopathy, cardiomyopathy, vision disorders, dysacusis and metabolic disorders. Despite numerous studies problems associated with mitochondrial diseases have not been completely solved yet.

Adult↗
PubMedDate not supplied

Effect of novel platinum complexes on survival rate of B16 and Cl S91 melanoma cells and oxidation stress in vitro.

BACKGROUND: The aim of study was the assessment of two novel platinum complexes and their cytotoxic effect on murine melanoma cells. The evaluation of possible oxidation stress in neoplastic cells coexistent with the effect of these cytostatic agents. MATERIAL AND METHODS: Two lines of murine metastatic melanomas: B16 and Cl S91 were selected for the experiments. Two platinum compounds were tested: cis-dichloro(dimethylsulphoxide)(1-beta-D-ribofuranosyl-1,2,4-triazol-3-carboxyamide)platinum (II) called Pt-complex 1 and chloro(dimethylsulphoxide)(1-beta-D-ribofuranosyl-1,2,4-triazol-3-carboxyamide)platinum (II) called Pt-complex 2. Melanoma cells were incubated for 24 hours with the platinum complexes mentioned above and culture medium at the concentration of 0.01, 0.05 and 0.5 mg/ml. Survival rate of melanoma cells, superoxide dismutase (SOD) activity, the concentration of thiobarbituric acid reactive substances (TBARS) and the content of conjugated dienes were estimated. RESULTS: A number of statistically significant relationships between analysed parameters were observed. Negative correlation ratios were found between increasing levels of analysed platinum complexes and the survival rate of the cells of both melanoma lines as well as between the survival rate of Cl S91 melanoma cells and the level of lipid peroxidation products and SOD activity. On the other hand, there was a positive correlation between increasing platinum complexes and the level of lipid peroxidation products as well as between the survival rate of B16 melanoma cells and SOD activity and the content of CD. CONCLUSIONS: Oxygen free radicals may play an important role in the development of melanoma and cytostatic agents disturb the balance between generation and removal of reactive oxygen forms in melanoma cells.

Animals↗