PubMed HealthSearch

Biomedical subjects

G Drexler

Publications and source records attributed to G Drexler.

At least 19 recordsLinked to original sources

An analysis of the equivalent dose calculation for the remainder tissues.

In the 1990 Recommendations of the International Commission on Radiological Protection, the risk-weighted quantity "effective dose equivalent" was replaced by a similar quantity, "effective dose." Among other alterations, the selection of the organs and tissues contributing to the risk-weighted quantity and their respective weighting factors were changed, including a modified definition of the so-called "remainder." Close consideration of this latter definition shows that it causes certain ambiguities and unexpected effects which are dealt with in the following. For several geometries of external photon irradiation, the numerical differences of two possible methods of evaluating the remainder dose from the doses to ten single organs, namely as arithmetic mean or as mass weighted average, are assessed. It is shown that deviation from these averaging procedures, as prescribed for those cases where a remainder organ receives a higher dose than an organ with a specified weighting factor, causes discontinuities in the energy dependence of the remainder dose and, consequently, also non-additivity of this quantity. These problems are discussed, and it is shown that, although the numerical consequences for the calculation of the effective dose are small, this unsatisfactory situation needs clarification. One approach might be to abolish some of the ICRP guidance relating to the appropriate tissue weighting factors for the remainder tissues and organs and to make other guidance more precise.

Adult

Effective dose--how effective for patients?

The question discussed in this paper is whether effective dose can reflect the risk to patients from radiological procedures and can be used, for example, to optimise procedures and compare risks of various methods, to define dose constraints, and to estimate the risks to individuals or populations attributed to medical exposures. This report demonstrates that the use of effective dose for patients could be misleading or even wrong due to inappropriate simplifications of the underlying biological mechanisms and inappropriateness of the weighting factors connected with the definition of effective dose for a given patient population. We show that the choice of the most meaningful quantities to express patient exposure depends strongly on the respective situation.

Bone Marrow

Effective dose and effective dose equivalent--the impact of the new ICRP definition for external photon irradiation.

In a recent recommendation, the International Commission on Radiological Protection substituted the effective dose equivalent, HE, with a similar quantity--the "effective dose," E--changing both the set of organs considered and the respective weighting factors. To quantify the impact of these changes, calculations of E and HE were performed for various photon energies and external irradiation geometries using a Monte Carlo code and mathematical anthropomorphic phantoms to which an esophagus was introduced for this purpose. For energies greater than 15 keV, E less than HE, the difference depending on photon energy and exposure geometry.

Female

The construction of computer tomographic phantoms and their application in radiology and radiation protection.

In order to assess human organ doses for risk estimates under natural and man made radiation exposure conditions, human phantoms have to be used. As an improvement to the mathematical anthropomorphic phantoms, a new family of phantoms is proposed, constructed from computer tomographic (CT) data. A technique is developed which allows any physical phantom to be converted into computer files to be used for several applications. The new human phantoms present advantages towards the location and shape of the organs, in particular the hard bone and bone marrow. The CT phantoms were used to construct three dimensional images of high resolution; some examples are given and their potential is discussed. The use of CT phantoms is also demonstrated to assess accurately the proportion of bone marrow in the skeleton. Finally, the use of CT phantoms for Monte Carlo (MC) calculations of doses resulting from various photon exposures in radiology and radiation protection is discussed.

Bone Marrow

[A field study to determine dosages in computed tomography].

122 CT scanners were studied in order to determine close values free in air on the axis of rotation during the most common types of examination. In addition, other exposure parameters were collected, which may be important in relation to dose to patients. The results showed a surprising variation in the dose values of CT examinations. It is concluded that it is not possible to estimate patient exposure reliably by using the information on exposure parameters supplied by the operator.

Humans

Calibration of a NaI spectrometer in dose equivalent quantities.

A sodium iodide spectrometer was calibrated in terms of dose equivalent quantities. The detector was irradiated with a known dose (dose equivalent) at 12 ISO x-ray qualities between 33 keV and 248 keV. The method of calibration is based on the relationship between the considered dose equivalent delivered by each x-ray quality and the linear combination of the number of counts in a set of six fixed energy ranges. This procedure allows for the evaluation of the effective dose equivalent, the whole-body dose equivalent and the testes dose equivalent for parallel irradiation with a maximum and minimum relative deviation from the reference values of +11% and -21%, respectively.

Calibration

Improved radioimmunoassay of melatonin in serum.

Melatonin was extracted from serum by using Baker reversed-phase C-18 columns. More than 99% of the applied melatonin was retained by the columns, and more than 97% was eluted from the columns in 300 microL of methanol. We then determined melatonin in the serum extract by a modification of a standard radioimmunoassay, using filtration instead of centrifugation to collect the [3H]melatonin-antibody complex precipitated by saturated ammonium sulfate. These modifications allow more rapid, accurate, and reproducible determination of melatonin than do previously published procedures.

Circadian Rhythm

A rapid and simple method for efficient coating of microtiter plates using low amounts of antigen in the presence of detergent.

Bio-Beads SM-2 have previously been used for the removal of non-ionic detergents from protein solutions. Addition of Bio-Beads SM-2 to detergent solubilized antigen significantly enhanced the immobilization of antigen to microtiter wells. Depending on the incubation time used 35-45% of the applied antigen could be immobilized to the microtiter wells. Using this method and a subsequent ELISA procedure it was possible to detect monoclonal antibodies in hybridoma supernatants after coating microtiter wells with 100 microliters of a solution containing 16 ng antigen/ml in the presence of 0.01% Triton X-100.

Allergy and Immunology

Experimental determination of conversion factors between organ doses and measured quantities for external photon irradiation.

For external photon radiation, conversion factors between organ doses and the quantities normally measured in radiation protection were determined experimentally using an anthropomorphic phantom and thermoluminescent dosimeters. The measurements were made for different energies and irradiation geometries. As normalization quantities, the exposure measured in free air and the dose equivalent in soft tissue measured at a personal dosimeter site were used. The results are compared with conversion factors calculated by other authors.

Female

[35S]tert.-butylbicyclophosphorothionate and avermectin bind to different sites associated with the gamma- aminobutyric acid-benzodiazepine receptor complex.

Low concentrations of avermectin B1a (AVM) stimulated the specific high affinity binding of [35S]tert.-butylbicyclophosphorothionate [( 35S]TBPT) to membranes from rat cerebral cortex in the absence or presence of chloride or bromide ions. In contrast, TBPT either weakly stimulaes or does not significantly influence the specific high affinity binding of [3H]AVM to the same membranes in the absence or presence of chloride ions, respectively. These results indicate that [3H]AVM and [35S]TBPT bind to different but closely associated binding sites.

Animals

Evidence for association of a high affinity avermectin binding site with the benzodiazepine receptor.

Avermectin B1a concentrations corresponding to the KD value of a specific high affinity binding site for [3H]avermectin B1a are sufficient to stimulate the specific high affinity binding of [3H]flunitrazepam to its receptors. This stimulation of [3H]flunitrazepam binding by low concentrations of avermectin B1a was enhanced by chloride ions and picrotoxinin and reduced by the GABA agonists THIP and PSA. The similar modulation by chloride ions, GABA agonists and picrotoxinin and the resistance against washing of membranes of avermectin B1a bound to its specific high affinity binding site or to the site modulating [3H]flunitrazepam binding indicate a close association of the specific high affinity binding site for [3H]avermectin B1a with the GABA-benzodiazepine receptor complex.

Animals

Properties of a high affinity binding site for [3H]avermectin B1a.

The specific high affinity binding of [3H]avermectin B1a was investigated in membranes from several rat brain regions. Binding occurred rapidly, was reversible and partially dependent on the presence of chloride ions in the incubation medium. Specific high affinity binding of [3H]avermectin B1a was partially inhibited by GABA receptor agonists and this effect was blocked by GABA receptor antagonists. Pentobarbital and etazolate inhibited, and picrotoxin, picrotoxinin and IPTBO stimulated high affinity binding of [3H]avermectin B1a. All these effects were influenced by the presence of chloride ions in the incubation medium. The results indicate that the high affinity binding site of [3H]avermectin B1a is associated with the GABA-benzodiazepine receptor-chloride ion channel complex.

Animals

Properties of [3H]flunitrazepam binding to different benzodiazepine binding proteins.

Membranes from cerebellum or hippocampus were incubated with various concentrations of [3H]flunitrazepam in the absence or presence of diazepam, Cl 218 872 or ethyl-beta-carboline-3-carboxylate (beta-CCE). After binding equilibrium of [3H]flunitrazepam had been established, the membranes were either filtered for determination of reversible binding or were irradiated with UV light for determination of irreversible binding. Irradiated membranes were then subjected to SDS-polyacrylamide gel electrophoresis and fluorography. Individual photolabeled proteins were identified, appropriate sections cut out of the gel, and the radioactivity in the gel pieces measured. The results indicate that [3H]flunitrazepam binding to individual benzodiazepine binding proteins and its inhibition by various drugs can be measured by the present technique and support previous evidence for the independent existence of various proteins irreversibly labeled by [3H]flunitrazepam and their possible association with different benzodiazepine receptors.

Animals

Irreversible binding of [3H]flunitrazepam to different proteins in various brain regions.

Irreversible photolabeling by [3H]flunitrazepam of four proteins with apparent molecular weights 51,000 (P51), 53,000 (P53), 55,000 (P55), and 59,000 (P59) was investigated in various rat brain regions by SDS-polyacrylamide gel electrophoresis, fluorography, and quantitative determination of radioactivity bound to proteins. On maximal labeling of these proteins, only 15-25% of [3H]flunitrazepam reversibly bound to membranes becomes irreversibly attached to proteins. Results presented indicate that for every [3H]flunitrazepam molecule irreversibly bound to membranes, three molecules dissociate from reversible benzodiazepine binding sites. This seems to indicate that these proteins are either closely associated or identical with reversible benzodiazepine binding sites, and supports the hypothesis that four benzodiazepine binding sites are associated with one benzodiazepine receptor. When irreversible labeling profiles of proteins P51, P53, P55, and P59 were compared in different brain regions, it was found that labeling of individual proteins varied independently, supporting previous evidence that these proteins are associated with distinct benzodiazepine receptors.

Animals