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Biomedical subjects

G E Bass

Publications and source records attributed to G E Bass.

15 recordsLinked to original sources

Bar-code technology for documenting administration of large-volume intravenous solutions.

The failure to properly document dispensing, administration, charging, and crediting of large-volume plain i.v. solutions in a hospital, along with the potential for using bar-code technology to reduce documentation discrepancies, was investigated. Portable bar-code scanners and preprinted bar-code labels were employed to identify large-volume plain i.v. solutions administered on two selected nursing units of a 1000-bed, private, not-for-profit hospital. Inservice training sessions were conducted to instruct hospital personnel in the use of the scanning equipment. Comparisons of patient statements and medication administration records for large-volume plain i.v. solutions established the level of documentation errors in the study hospital. The causes of these errors were traced to three primary sources: (1) failure to document administration of a solution to a patient (38%), (2) failure to credit patients for i.v. solutions returned to the pharmacy (37%), and (3) administration of a solution to a patient other than the patient for whom the solution was dispensed (25%). Accountability for large-volume plain i.v. solution charges to patients was improved by 19% using bar-code technology. The pharmacy manager desiring to employ bar-code technology should determine convenient methods for applying bar-code labels to solutions and for scanning the bar codes, as well as provide programming that can compensate for erroneous scans.

Documentation

Substrate irradiation stimulation of the in vitro lactate-pyruvate interconversion reactions mediated by lactic dehydrogenase.

Experimental studies of the Comorosan effect are presented for the LDH-mediated interconversions of lactate and pyruvate. Consistent with the findings of the Comorosan group, the rate of the lactate/LDH reaction was found increased for crystalline lithium lactate irradiated with green light for times t comprising the manifold t = 15+30n sec, n = 0,1,2... However, no upper limit to the number of activating times was encountered although the Comorosan group has always obtained only six such activations. The pyruvate/LDH reaction rate was found enhanced for crystalline sodium pyruvate irradiated t = 5+30n sec. Sharpness of activations for 5-sec and 65-sec irradiated samples was investigated and found to occur only within approximately +/- 0.5 sec of 5.0 and 65.0 sec, slightly broader than the +/- 0.15-sec peak reported by Comorosan for the 5-sec signal. The data contribute to the credibility of the phenomenon but reveal sensitivity of some properties to individual laboratory or procedural factors. The first support is provided for the "discriminating function" component of Comorosan's metabolic control hypothesis.

L-Lactate Dehydrogenase

Inhibition of Streptococcus faecalis by long chain aliphatic monoamines: quantitative structure-activity studies.

Primary, secondary, and tertiary long chain aliphatic amines were synthesized, and their activity against Streptococcus faecalis was determined. Quantitative structure-activity analyses were carried out based on the Hansch extrathermodynamic model, using partition coefficients, CMC's, quantum mechanical charges on the amine nitrogen, and the Taft steric parameter. The best correlations were obtained with the CMC. Steric properties of the ammonium head become important for tertiary amines. The structural feature of these compounds that dominates biological activity is the length of the alkyl tail. Ammonium head substituents are of only secondary importance.

Amines

The Comorosan effect: single- and double-blind studies on the urea/urease system.

Characteristic alterations of enzymatic reaction rates by irradiation of the crystalline substrates for fixed periods with an Hg vapor lamp were reported by Comorosan in 1969. Results of single- and double-blind studies reported here support the validity of the key features of this striking and potentially important phenomenon.

Ammonia

Synthesis and CMC determination of a series of aliphatic diamines.

Fifteen aliphatic diamines substituted with one long alkyl chain (C10-C18), a varying number of methylene groups separating the nitrogens (two to six), and various aliphatic substituents were synthesized and their CMC's were determined. While the length of the long alkyl chain plays a dominant role in influencing the CMC, the other N-alkyl substituents as well as the length of the methylene chain separating the two nitrogens of the diamine are significant factors.

Chemical Phenomena

Quantitative studies of in vitro inhibition of Streptococcus mutans plaque formation by organic amines.

The abilities of five, long-chain aliphatic monamines to inhibit the deposition of plaque formed by Streptococcus mutans on stainless-steel wires were determined. The compounds along with their relative inhibitory potencies that were studied are N-methyltetradecylamine, hexadecylamine, dodecylamine, N-ethyldodecylamine, and octylamine. Activities are compared with critical micelle concentrations, partition coefficients, and quantum mechanical charges on the amine nitrogens.

Amines

Structure-activity studies on inhibition of Streptococcus mutans by long-chain aliphatic diamines.

A series of long-chain aliphatic diamines, R1R2N(CH2)mNR3-CnH2n+1' was tested for in vitro inhibition of Streptococcus mutans. In general, high activity was found for all analogs with alkyl chains containing 14 to 18 carbons. The nature of the substituents on the remainder of the alkylenediamine were of secondary importance. N-hydroxyethyl substituents tend to decrease activity. Good correlations of activity with quadratic functions of diamine critical micelle concentrations were obtained.

Chemical Phenomena