Biomedical subjects
Publications and source records attributed to G E Busch.
The ability to directly measure and evaluate ultrafast processes with unprecedented time resolution and reliability has greatly extended our knowledge about the kinetics of primary processes in chemistry and allied physical and biological sciences. Improvements in the reliability and versatility of picosecond techniques should lead to an increase in the experimental information about basic interactions in atomic and molecular systems.
We have excited detergent-solubilized bovine rhodopsin at room temperature with 530-nm light pulses from a mode locked laser, and have observed the appearance and decay of a transient species that absorbs more strongly at 560 nm than does ground-state rhodopsin. Our data show that the absorbing intermediate appears in a time that is at least as short as the experimental resolution (about 6 psec) and decays with a life time of about 30 nsec. The extremely fast risetime supports the hypothesis that prelumirhodopsin is the product of the primary photoprocess.