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Biomedical subjects

G Engel

Publications and source records attributed to G Engel.

At least 109 records · Page 6Linked to original sources
PubMed1979

Acid phosphatase.

Acid phosphatase is a ubiquitous lysosomal enzyme that hydrolyses organic phosphates at an acid pH. Although the postpuberteral prostatic epithelial cell contains a uniquely high concentration of acid phosphatase, cellular components of bone, spleen, kidney, liver, intestine, and blood also contain this enzyme. The discovery that prostatic carcinoma cells often retain a high concentration of acid phosphatase characteristic of the normal postpubertal gland led to the recognition of the first clinically useful tumor marker. Recognition that the serum of patients with prostatic malignancy frequently contains an increased concentration of this enzyme has resulted in persistent efforts to identify the source, to accurately quantitate the level of serum acid phosphatase, and to determine the clinical significance of those levels. A variety of enzymatic and immunologic techniques have been employed to measure acid phosphatase. In the past, various substrates and inhibitors were utilized to increase specificity and sensitivity. Emphasis has now shifted to the development of radioimmunoassay and counterimmunoelectrophoresis in an attempt to enhance those parameters. Judgment of their efficacy awaits further testing and evaluation. The clinical significance of normal and abnormal serum acid phosphatase is constantly being reevaluated. In order to maximize the value of laboratory measurements, the clinical and pathologic status of the patient, the techniques employed in obtaining and storing the blood sample and the procedures used in analysis must be known and considered. Traditionally, the serum prostatic acid phosphatase has been thought to originate in the prostatic cancer cell and has been used to stage the disease. Until recently, elevated serum values have been accepted as an indication of extraprostatic disease, and were thought to rule out lesions confined to the prostate. The elevation of acid phosphatase levels in patients with disseminated disease or the failure of elevated levels to return to normal with treatment have been assumed to indicate a poor prognosis. However, unequivocal documentation of the validity of these statements is not available. Newer immunologic techniques for measuring acid phosphatase may significantly alter our current concept of its role as a tumor marker.

Acid Phosphatase↗
PubMed1978

Penile blood pressure in the evaluation of erectile impotence.

Identification of specific etiologies of erectile impotence is hindered by a paucity of objective criteria needed to segregate organic from psychogenic varieties and to define specific organic causes. Simplified measurement of penile blood pressure was performed with a digital cuff and a portable ultrasound Doppler system for flow detection. An index relating penile-to-brachial systolic blood pressure was generated, and the results were compared in patients with impotence versus patients with normal erections. The mean indices for the two groups were significantly different (P less than 0.05). The penile-to-brachial index provides an additional diagnostic tool in the evaluation of potency, allowing us to identify those patients with decreased penile blood flow, and may be helpful in selecting patients for revascularization procedures.

Blood Pressure↗
PubMed1978

Ureterolymphatic backflow.

A case is reported of apparent radiologic demonstration of a communication between a completely obstructed distal ureter and its draining lymphatics in a patient with invasive bladder tumor involving the ureteral orifice.

Aged↗
PubMed1977

[Investigations on the production of mycotoxins and their quantitative analysis. VI. Citreoviridin (author's transl)].

UV-densitometric and fluorodensitometric evaluations of the mycotoxin citreoviridin on thin-layer plates are described. The UV maximum is at 360 nm on thin-layer plates and at 388 nm in ethanolic solution. Its emission maximum has been found to be at 525 nm both in chloroform and on thin-layer plates. The two procedures are similar in their sensitivity. Since the UV-densitometric evaluation is more reliable, it is preferred to fluorodensitometric procedure.

Chromatography, Thin Layer↗
PubMed1975

[Investigations on the production of mycotoxins and their quantitative evaluation. I. The production of penicillic acid by Penicillium cyclopium (author's transl)].

A convenient thin-layer chromatographic screening procedure for the analysis of mycotoxins produced by Penicillium cyclopium is described. The production of penicillic acid is followed during a growth period of 44 days at two different temperatures. Quantitative evaluation is performed by UV densitometry at 234 nm. A biological profile is recorded showing the different ratios of four metabolites produced by Penicillium cyclopium and the definite effect of the growth temperature on the formation of penicillic acid in relation to the other metabolites.

Caproates↗