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Biomedical subjects

G F Grant

Publications and source records attributed to G F Grant.

8 recordsLinked to original sources
PubMed1989

Effect of some 4-alkyl-5-dimethylamino-1-phenyl-1-penten-3-one hydrochlorides and related compounds on respiration in mouse liver mitochondria.

5-Dimethylamino-1-phenyl-1-penten-3-one hydrochloride (1a) has high inhibiting properties in mitochondria isolated from mice liver. Substitution at the 4-methylene group of 1a by different alkyl substituents lead to compounds with wide variation in respiration-inhibiting properties. No correlations were found between either the Charton steric parameter (v), Taft inductive value (f*) or fragmental constant (f) of the substituents at position 4 of the Mannich bases with inhibition of respiration. However the biological results suggested two receptor sites were present in the mitochondria which may interact with the Mannich bases namely a common receptor for all compounds and in addition a narrow hydrophobic binding area which can accommodate a n-butyl or higher alkyl groups which are present in some of the compounds.

Animals↗
PubMed1969

Synthesis of bacterial flagella: chromosomal synchrony and flagella synthesis.

Synchronous cultures of Bacillus subtilis 168 M were obtained from light-density spores germinated at 46 C and grown at 37 C. This procedure synchronizes both cell division and chromosome replication. The chromosome synchrony was demonstrated by using transformation to measure changes in marker frequency during the cell cycle. The synthesis of two enzymes and of bacterial flagellar protein was also followed. All of the proteins were found to be synthesized continuously with an abrupt doubling in the rate of synthesis at a specific time in the cell cycle. The time at which the doubling occurred for each enzyme corresponded to the time at which the structural gene for the enzyme was replicated. The doubling of the rate of flagella synthesis corresponded to the time of replication of the hisA1 gene. We conclude that the genetic locus for the factors involved in the rate-limiting steps in flagella synthesis are located on the genetic map near the hisA1 locus.

Bacillus subtilis↗
PubMed1969

Synthesis of bacterial flagella. II. PBS1 transduction of flagella-specific markers in Bacillus subtilis.

The linkage relationship of mutants involved in the synthesis of flagella was determined by PBSl transduction. Mutants that affect the structure of flagellin (hag) and temperature-sensitive mutants (flaTS) that produce flagella when grown at 37 C but not when grown at 46 C were examined. All of the mutants were found to be linked to the hisA1 marker. The flaTS mutants fell into three clusters. Group A contained the majority of mutants which were loosely grouped around the hag locus. Group B mutants were segregated from the hag locus and appeared closely linked to the phage adsorption site gene (gtaA), and group C was only loosely linked to hisA1 and thus far contains only one mutant. A flagella locus (ifm) affecting both the degree of motility and level of flagellation was shown to map near group A. Mutants affecting motility (mot) were not linked to hisA1 by PBSl transduction. Several markers previously shown to link to hisA1 were ordered with respect to hisA1 and the flagellar genes.

Antigens↗
PubMed1968

Use of radioactive antibodies for characterizing antigens and application to the study of flagella synthesis.

A simple rapid immunochemical procedure has been developed which provides information about the qualitative and quantitative nature of antigens. It involves the use of purified radioactive ((125)I-labeled) antibodies. The amount of antibody bound to the antigen is determined by filtering the mixture through diethylaminoethyl (DEAE)-cellulose paper. All of the antigen, as well as the antibody complexed with it, is trapped on the paper, whereas free antibody is removed by repeated washing. This technique has been applied to the study of three immune systems, bovine serum albumin, Escherichia coli tryptophan synthetase B protein, and Bacillus subtilis flagella. The results obtained by the DEAE-antibody binding technique were comparable, in terms of sensitivity, specificity, and accuracy, to data obtained by microcomplement fixation and precipitin methods. The assay was used to measure the kinetics of flagella regeneration in B. subtilis.

Antibodies↗