Biomedical subjects

G F Huang

Publications and source records attributed to G F Huang.

At least 19 recordsLinked to original sources

PubMed2002

Elbow load with various forearm positions during one-handed pushup exercise.

This is the first study of the one-handed pushup, and tries to show the effects of forearm rotations. Previous studies of elbow loading have focused on passive loading and small loads, because data from large loads during active exercise is not easy to obtain. In order to investigate the biomechanical impact of hand position on the elbow and the potential trauma mechanisms of outstretched elbow, joint loading across the elbow was analyzed for three forearm rotational positions, neutral, 90 degrees internal rotation and 90 degrees external rotation. Both kinematic and kinetic data were collected from eight volunteers by the Motion Analysis System and a Kistler Force Plate. Statistical analysis of the data delineates the relationship between elbow joint load and hand rotational position during one-handed pushup, and also provides useful biomechanical information for this challenging exercise. The axial and valgus stresses and forces are the major concerns. The peak axial forces exerted on the elbow joint averaged 65 % of the body weight when the hand position was neutral, and was significantly reduced with the hand rotated either internally or externally. The peak valgus shear force with the hand externally rotated was 50 % greater than the other two positions. Thus, outward rotation of the hand is a stressful position that should be avoided during one-handed pushup exercise or forward falls with outstretched hands in order to reduce the risk of elbow injuries.

Adolescent↗

PubMed2001

Uncharged thioflavin-T derivatives bind to amyloid-beta protein with high affinity and readily enter the brain.

In vivo assessment of the beta-sheet proteins deposited in amyloid plaques (A beta peptide) or neurofibrillary tangles (tau protein) presents a target for the development of biological markers for Alzheimer's disease (AD). In an effort to develop in vivo beta-sheet imaging probes, derivatives of thioflavin-T (ThT) were synthesized and evaluated. These compounds lack the positively charged quaternary heterocyclic nitrogen of ThT and are therefore uncharged at physiological pH. They are 600-fold more lipophilic than ThT. These ThT derivatives bind to A beta(1-40) fibrils with higher affinity (Ki = 20.2 nM) than ThT (Ki = 890 nM). The uncharged ThT derivatives stained both plaques and neurofibrillary tangles in post-mortem AD brain, showing some preference for plaque staining. A carbon-11 labeled compound, [N-methyl-11C]6-Me-BTA-1, was prepared, and its brain entry and clearance were studied in Swiss-Webster mice. This compound entered the brain at levels comparable to commonly used neuroreceptor imaging agents (0.223 %ID-kg/g or 7.61 %ID/g at 2 min post-injection) and showed good clearance of free and non-specifically bound radioactivity in normal rodent brain tissue (brain clearance t(1,2) = 20 min). The combination of relatively high affinity for amyloid, specificity for staining plaques and neurofibrillary tangles in post-mortem AD brain, and good brain entry and clearance makes [N-methyl-11C]6-Me-BTA-1 a promising candidate as an in vivo positron emission tomography (PET) beta-sheet imaging agent.

Alzheimer Disease↗

PubMed2001

Effect of elbow flexion on upper extremity impact forces during a fall.

OBJECTIVE: The overall objectives are to develop a biomechanical model for a simulated fall with outstretched hand. DESIGN: Cross-sectional study involving young healthy volunteers in a university research laboratory setting. BACKGROUND: Little is known about the factors which influence fracture risk during a fall on outstretched hand. METHODS: A group of 11 male subjects volunteered for this investigation. A set of eight reflective markers was placed bilaterally on selected anatomic landmarks. Subjects were suspended with both elbows extended and wrists dorsiflexed, preparing to impact the ground and force plates from two different fall heights: 3 and 6 cm. Two different postures for the elbows were employed. In the elbow extension experiment, the elbows were extended at all times. In the elbow flexion experiment, the elbows were extended at impact, but then flexed immediately, as though in the initial downward phase of a push-up exercise. RESULT: Increasing the fall height significantly increased the upper extremity axial forces by 10% and 5%. No significantly different differences were found in the axial forces applied to the wrist, elbow or shoulder between the elbow flexion and elbow extension trials, but the elbow mediolateral shear force was 68% larger (P=0.002) in the extension trials. CONCLUSIONS: Performing an elbow flexion movement at impact reduces the first peak impact force value and postpones the maximum peak value. Although changing the fall arrest strategy from elbow extension to elbow flexion did not affect the peak impact force on the hand, it did require substantially greater elbow and shoulder muscle strengths. RELEVANCE: This paper yields insights into how the physical demands of arresting mild falls may relate to upper extremity muscle capacity, joint dislocation and bony fracture.

Accidental Falls↗

PubMed2001

The relationship between radiologic interpretation and root tip fracture during tooth extraction performed by junior clinicians.

OBJECTIVES: The purpose of this study was to analyze the relationship between root tip fracture and radiologic interpretation of root morphology on periapical radiographs during third molar extraction performed by junior clinicians. METHODS: Before tooth extraction, periapical radiographs of 107 patients with 116 third molars were evaluated by junior clinicians, all with less than 5 years of clinical experience. Radiologic interpretations of root morphology-including number, curvature, fusion, and accessory root-were recorded before each extraction. The clinicians were also asked to estimate the possibility of root fracture on a Visual Analogue Scale before the procedure. The exact morphology of the extracted teeth was recorded after the extraction for the purpose of comparison. RESULTS: Twenty-nine of 116 teeth extracted were not correctly interpreted in at least 1 of the morphologic categories surveyed. The average expected fracture rate of nonfractured teeth was 23.1%, whereas the average expected fracture rate of fractured teeth was a significantly higher 50.3%. Misinterpretation of root morphology on radiographs decreased with increased clinical experience. Senior residents had the lowest misinterpretation and fracture rate. Logistic regression analysis showed that fracture is most closely related to the estimated fracture rate (4.95) and is also significantly related to underestimation of root curvature (0.95; 24.56 with 2 df of chi-square, P = .0001). CONCLUSIONS: Misinterpretation of root morphology on radiographs occurred in 25% of the teeth. Root curvature was the most misinterpreted item studied. Fracture was most closely related to the estimated fracture rate. Junior clinicians in this study expected that only 50% of the fractured teeth would fracture, reflecting a general underestimation of root tip fracture. Further study should be performed to evaluate how to increase the accuracy of root curvature interpretation.

Clinical Competence↗

PubMed2001

Co-composting of pig manure with leaves.

The increase in the market price of sawdust makes it a less attractive bulking agent for pig manure composting. Hence, it was the aim of this project to evaluate the feasibility of co-composting pig manure with leaves with special emphasis on its effects on compost maturity and quality. Two piles were prepared with one pile (Pile A) just constituted of pig manureand sawdust at a mixing ratio of 3:2 (w/w, fresh weight), while the other one (Pile B) with pig manure, sawdust and leaves at 3:1:1 ratio (w/w, fresh weight) to achieve a C/N ratio of 30. Each 8 m3 heap was turned and mixed every 3 days to provide sufficient aeration and the moisture content was kept at 60-70% (w/w) throughout the composting period. After 49 days of composting, dissolved organic carbon (DOC), soluble NH4-N(solid), C/N(aqueous) and C/N(aqueous) of pile A decreased to <5%, 400 mgkg(-1), 20 and 6, respectively, indicating a high degree of maturity, while pile B with leaves required only 35days. Seed germination index (GI) of pile A increased to 66.5% at day 49, while pile B to 52.4% at day 35, which was higher than the phytotoxin-free level of 50% recommended for agricultural use. This demonstrated that the addition of leaves enhanced the humification process and shortened the time required for maturation and stabilization of pig manure composting. Therefore, it is recommended to co-compost pig manure with leaves to provide a means to reutilize this waste and in the same time to reduce the dependence on sawdust as a buLking agent.

Animals↗

PubMed2001

Synergistic effect of Nd:YAG laser combined with fluoride varnish on inhibition of caries formation in dental pits and fissures in vitro.

BACKGROUND AND PURPOSE: Although the effectiveness of neodymium yttrium aluminum garnet (Nd:YAG) laser and fluoride anticaries treatment has been established, most previous studies focused on smooth tooth surfaces. We evaluated the anticaries effects of Nd:YAG laser combined with fluoride varnish (Duraphat) on caries-susceptible pit and fissure areas. METHODS: A total of 36 noncarious molars were treated with either a Nd:YAG laser (2.5 W, 6 sec) followed by fluoride varnish, Nd:YAG laser only, fluoride varnish only, or no treatment (control). Artificial carious lesions were created to assess the acid resistance of enamel after treatment. Undecalcified successive tooth slices were made. Percentage lesion formation, lesion length, and lesion depth were evaluated using polarized light microscopy. RESULTS: The Nd:YAG laser enhanced the resistance of dental enamel to acid challenge. However, Nd:YAG laser alone was not as effective as the Nd:YAG laser combined with fluoride varnish, especially for the treatment of pits and fissures. Nd:YAG laser treatment combined with fluoride varnish inhibited 43% of lesions at pits and fissures and 80% of lesions on smooth surfaces compared to no treatment. Carious lesions had shallower depth and shorter length. No carious lesion extended beyond the dentinoenamel junction in either laser-treatment group. CONCLUSIONS: A synergistic effect on dental caries prevention in pit and fissure areas and on the smooth surfaces of the tooth can be achieved by applying Nd:YAG laser followed by fluoride varnish.

Dental Caries↗

PubMed2001

TiO2-assisted photo-catalysis degradation process of dye chemicals.

The photo-catalytic degradation pathway and degradation products of methylene blue, rhodamine B, methyl orange, and malachite green in aqueous TiO2 suspension irradiated by high pressure mercury lamp by means of UV-visible absorption spectra and ion chromatography were investigated. The photo-catalysis degradation of dye solutions with charges was greatly effected by pH value owing to the electrostatic model. The photo-degradation rate of dyes anion increased with the decrease of pH value, in contrast, the photo-degradation rate of dyes cation increased with the increase of pH value. And the absorption peaks diminished with a blue shift. After illuminated for 30 minutes, a part of dye chemicals were completely mineralized and transferred into inorganic species including chloride ion, ammonium ion, nitrate ion, sulfate ion. And the addition of 100 mmol/L H2O2 promoted the formation of inorganic species. In this study, the quantity of ammonium ion was much more than that of nitrate ion. That indicated the formation of nitrate is from ammonium. The purification rate of COD in four kinds of dye solution was 71.7%-88.7%. The decrease of COD of dyes solution implies the feasibility of the environmental application of photo-catalyzed process.

China↗

PubMed2001

Enhanced photo--catalytic activity of gold ion and gold modified.

The gold ion modified TiO2 was prepared by means of sol-gel whereas gold deposited TiO2 was prepared by means of photo-reduction. The physical properties were influenced significantly by the presence of gold ion or gold. The enhanced photo-activity of gold modified TiO2 was quantified in terms of methylene blue degradation. The presence of gold ion in TiO2 lattices or gold on TiO2 surface enhanced their photo-activity. The optimum molar content of gold ion doping and gold deposition all was 0.5%. The first-order rates constants of gold modified TiO2 was more than that of pure TiO2, and decreased by increasing the content of gold ion and gold when their contents were more than 0.5%. Gold ion doped in TiO2 lattices was more effective to enhance the photo-activity than gold on TiO2 surface. Moreover, the relationship between physical properties, chemical properties and photo-activity has been discussed.

Binding Sites↗

PubMed2001

Nitrogen transformations during pig manure composting.

Composting is now suggested as one of the environmentally and friendly alternative method for disposal of solid organic wastes, as it leads to minimization, stabilization, and utilization of organic waste. Transformations of nitrogen were investigated in co-composting of pig manure with different amendments, such as sawdust and leaves. Samples were analyzed for pH, total-N, soluble NH4-N, soluble NO3-N and soluble organic-N. The total-N increased after 63 days of composting, as well as the soluble NO3-N and soluble organic-N. Soluble NH4-N increased significantly and showed peak values at day 7, thereafter decreased sharply and gradually to lower levels. Seed germination index (GI) showed that co-composting of pig manure with sawdust reached maturity after 49 days of composting, while co-composting of pig manure with sawdust and leaves required shorter time for 35 days. Soluble NH4-N was significantly negatively (P < 0.05), while soluble NO3-N and soluble organic-N were significantly positively (P < 0.05), correlated with seed germination index (GI). Addition of leaves in co-composting of pig manure with sawdust had no significant impacts on nitrogen transformations, but it was beneficial for maturity of pig manure compost.

Animals↗

PubMed2000

Gait analysis and energy consumption of below-knee amputees wearing three different prosthetic feet.

This study scientifically measures the dynamic gait characteristics and energy consumption of 16 male below-knee amputees, eight vascular and eight traumatic, while wearing solid ankle cushion heel (SACH), single axis and multiple axis prosthetic feet via six-camera motion analysis, metabolic measurement cart and heavy-duty treadmill. Subjective results are additionally determined via questionnaire after testing. Motion analysis showed statistically significant differences at P<0.05 between the SACH, single axis and multiple axis foot in the velocity, cadence, stride length and single limb stance. Significant differences were found in energy consumption between the traumatic and vascular groups, and significant changes in walking under different speeds and different inclines. Results provide quantitative and qualitative information about the dynamic performance of the various feet, which can be helpful in prescribing the optimal prosthetic foot for individual amputees.

Adult↗

PubMed2000

Resting dental plaque pH values after repeated measurements at different sites in the oral cavity.

Plaque pH measurements can be used to detect individual's caries susceptibility and the cariogenic potential of ingested foods. However, repeated measurements with a touch electrode at a single site may affect the metabolic state of the dental plaque and result in changes of its inherent pH values. The purposes of this study were to evaluate the influence of repeated measurements of resting plaque pH values and to determine the pH values at different interdental sites. Eleven dental students participated in the study. The dental plaque was built up after thorough oral hygiene care. The subjects were then instructed to maintain a normal diet but to refrain from any oral hygiene care for the next 48 hours. Measurement of plaque pH was performed with an antimony electrode at 6 interproximal sites, including central spaces between the upper and between the lower central incisors, and the area between the second premolar and the first molar in each quadrant. At each tested site, measurements were taken 5 times at 0, 10, 30, 45 and 60 min. The results showed that the plaque pH became slightly alkaline at all the tested locations after repeated measurements. However, only the lower incisor area showed a significant change in pH values. Interdental plaque on the lower arch showed higher pH values than did that on the upper arch. There was no significant difference between the pH values on the right and left corresponding sites as determined using the Wilcoxon signed ranks test.

Adult↗

PubMed2000

Nanospheres of biodegradable polymers: a system for clinical administration of an anticancer drug paclitaxel (Taxol).

INTRODUCTION: Paclitaxel is a natural antineoplastic drug. It has been approved by the Food and Drug Administration (FDA) for the treatment of various cancers, especially ovarian and breast cancers with the best effect over other anticancer agents. Due to its high hydrophobicity however, an excipient such as Cremophor EL has to be used, which causes additional serious side effects. This study was intended to investigate the feasibility to apply nanospheres of biodegradable polymers as an alternative system to achieve best results with least side effects. MATERIALS AND METHODS: FDA-approved biodegradable polymers such as poly(D, L-lactic-co-glycolic acid) (PLGA) and poly(D, L-lactic acid) (PLA) were used to fabricate paclitaxel-loaded nanospheres by the solvent extraction/evaporation technique. The nanospheres fabricated in various conditions were characterised by laser light scattering for size and size distribution, scanning electron microscopy (SEM) and atomic force microscopy (AFM) for surface morphology, X-ray photoelectron spectroscopy (XPS) for the chemical structure of the surface. The drug encapsulation efficiency (EE) and the in vitro release kinetics were measured by HPLC. RESULTS: The products ranged from 300 nm to 700 nm with low polydispersity. The in vitro drug release can last more than 6 months at an approximately constant rate after an initial burst. The release kinetics can be fully controlled by the material used and the fabrication conditions. CONCLUSIONS: Nanospheres of biodegradable polymers can be an ideal carrier for pacitaxel to eliminate the side effects caused by the excipient currently used in clinical administration. The technique developed can also be applied to other anticancer drugs.

Antineoplastic Agents, Phytogenic↗

PubMed1996

Nasopharyngoscopic evaluation and cephalometric analysis of velopharynx in normal and cleft palate patients.

The velopharyngeal dimensions and velopharyngeal functions of 58 patients having a cleft lip and palate were compared with those of 30 normal noncleft subjects. The patient group received perceptual speech assessment, nasopharyngoscopic examination, and lateral cephalogram, and was classified accordingly into three categories: velopharyngeal competency, borderline velopharyngeal competency, and velopharyngeal incompetency. The results indicated that (1) the need ratio differs significantly among the three cleft palate patient groups and (2) when compared to the control group, the velopharyngeal incompetency group has an excessive need ratio, narrower posterior pharyngeal wall, narrower linear dimensions in pharyngeal depth, and shorter velar and hard-palate length. The clinical implication and application of the findings are discussed.

Adolescent↗

PubMed1995

Changes of dentinal tubules following fluoride iontophoresis.

Fluoride(F) iontophoresis is preferred over topical application to provide long lasting dentin desensitization. The purpose of this study was to evaluate the changes in dentinal tubules following fluoride iontophoresis under SEM observation. Teeth were divided mainly into 3 groups: controls, NaF treated teeth with iontophoresis and those without inotophoresis. A battery operated device was used to apply electric current to teeth. All the teeth were prepared for scanning electron microscopy after treatments. Fluoride treatment, either non-iontophoretic or iontophoretic, produced granular precipitation in dentinal tubules and reduced tubular diameter. In 5-minute non-iontophoretic NaF treated teeth, the depth of precipitate penetration was 75 microns on average where the granules were small and sparse. In the 5-minute iontophoretic NaF treated teeth, the average penetration of precipitation reached 250 microns in one application whereas in three applications to teeth the average depth of penetration reached 830 microns. Fluoride iontophoresis resulted in obstruction of dentinal tubules. Both the frequency and duration od treatment under constant current and voltage affected the size of particles, the form of precipitation and the depth of penetration. Larger particle size and deeper fluoride penetration were observed in iontophoresis treated teeth compared to non-iontophoresis treated teeth. Therefore, repeated fluoride iontophoresis treatment is suggested to ensure long lasting densensitization effects.

Dentin↗

PubMed1994

The parabrachial area: electrophysiological evidence for an involvement in visceral nociceptive processes.

1. Neurons (n = 142) were recorded with extracellular micropipettes in the parabrachial (PB) area, in the anesthetized rat, some of them being antidromically driven from the centralis nucleus of the amygdala (Ce). The spontaneous activity of these neurons was low, (10th percentile < median frequency < 90th percentile; 0.01 < 0.3 < 11 Hz), and the activity of a very high proportion of the PB neurons (89%, 127/142) were affected by mechanical or thermal cutaneous stimuli almost exclusively in the noxious range; most of them were activated and received inputs from A delta and/or C fibers. 2. A majority of the PB neurons (66%, 93/142) were affected by visceral stimuli (bradykinin intraperitoneal and/or colorectal distension), almost exclusively in the noxious range; all of them (except one) were also affected by cutaneous noxious stimuli. The remaining neurons not affected by these visceral stimuli were only activated by cutaneous noxious stimuli (n = 35) or completely unresponsive (n = 14; i.e., only 10% of the whole population). 3. Thirty-five percent (49/142) of the whole population of PB neurons responded to bradykinin intraperitoneal and/or to strong colorectal distension with an intense and sustained increase of discharge. The response to bradykinin and to colorectal distension was often dissociated, i.e., the activation was often produced by only one of the stimuli the other being ineffective or inhibitory. The intensity of responses to visceral noxious stimuli was between 5-45 Hz with a mean value of 20 +/- 2 (SE) Hz (n = 49). The visceral activated neurons exhibited a clear capacity to encode the colorectal distension in noxious range: 1) the stimulus-response function was almost always positive and monotonic; 2) as for the individual curves, the slope of the mean curve progressively increased up to the highest interval of pressure tested (100-125 mmHg); and 3) the threshold for neuronal response to colorectal distension was between 25-100 mmHg with a mean pressure threshold of 56 +/- 24 (SD) mmHg (n = 19). Viscerosomatic convergence was observed for 84% of these neurons: they were also activated by thermal and/or mechanical cutaneous noxious stimuli while only 16% of them were activated only by visceral noxious stimulation. 4. Thirty-one percent (44/142) of the whole population of PB neurons were inhibited by both strong colorectal distension and intraperitoneal bradykinin (82%) or only by one of the stimuli being unresponsive to the other (18%).(ABSTRACT TRUNCATED AT 400 WORDS)

Afferent Pathways↗

PubMed1993

Intravenous morphine depresses the transmission of noxious messages to the nucleus centralis of the amygdala.

It has recently been demonstrated that the nucleus centralis of the amygdala contains numerous neurons specifically driven by noxious stimuli. The aim of the present study was to investigate the effect of i.v. morphine on responses of neurons located in the nucleus centralis of the amygdala to noxious mechanical or thermal stimuli. It was observed, in halothane-anesthetized rats, that i.v. morphine caused a marked depression of responses induced by noxious thermal (waterbath, 50 degrees C) and mechanical (pinch) stimuli and caused a moderate depression of spontaneous activity in a dose-related (1, 3, 9 mg/kg) and naloxone reversible fashion. The ED50 value was 1.2 and 9 mg/kg for i.v. morphine for the evoked activity and spontaneous activity, respectively. The strong depressive effect of morphine on evoked activity probably reflects a direct action of this drug at both spinal and parabrachial levels. These results could account, at least in part, for the effect of morphine on the emotional-affective aspects of pain.

Amygdala↗

PubMed1993

Morphine depresses the transmission of noxious messages in the spino(trigemino)-ponto-amygdaloid pathway.

We had recently demonstrated that a subregion of the parabrachial area relays nociceptive information in the spino(trigemino)-ponto-amygdaloid pathway. The aim of the present study was to investigate, in halothane-anesthetized rats, the effect of i.v. administration of morphine on nociceptive specific neurons located in the parabrachial area with some cells being antidromically driven from the amygdala. Morphine induced a marked depression of responses elicited by noxious thermal stimuli (waterbath, 50 degrees C) dose relatedly (1, 3, 9 mg/kg) and naloxone reversibly, with an ED50 = 1.8 mg/kg. This effect of morphine probably not only reflects the direct action of this drug at the spinal level, but could also involve a direct action at the parabrachial level. These results could account at least in part for the effect of morphine on the emotional-affective aspects of pain.

Amygdala↗

PubMed1992

Nucleus centralis of the amygdala and the globus pallidus ventralis: electrophysiological evidence for an involvement in pain processes.

1. Neurons (n = 177) were recorded with extracellular micropipettes in and around the nucleus centralis of the amygdala (Ce), in anesthetized rats. The spontaneous activity of these neurons was variable (0.25 less than 3 less than 35 Hz, n = 175; 10th percentile less than median less than 90th percentile). A majority (80%) of these neurons were excited or inhibited exclusively or preferentially by noxious stimuli. These units were separated into two groups: 1) a group of neurons excited by noxious stimuli (46% of the whole population) and 2) a group of neurons inhibited by noxious stimuli (34% of the whole population). 2. The receptive fields of both groups of neurons were very large: in about one-half the cases the neurons responded similarly from all parts of the body, and in the other cases the responses were greater when the stimuli were applied to a restricted part of the body. 3. Seventy-seven percent of the excited neurons had responses of relatively high magnitudes. In this group, most cells (75%) were exclusively driven by noxious stimuli; the others (25%) were preferentially activated by noxious stimuli. These neurons responded to mechanical (pinch or squeeze) and/or thermal (water bath or water jet greater than 44 degrees C) noxious stimuli with a marked and sustained activation. 4. Sixty percent of the inhibited neurons had a marked decrease of activity in response to noxious stimuli. In this group, most of them (81%) were exclusively inhibited by noxious stimuli, whereas the remainder (19%) were preferentially inhibited by noxious stimuli. These neurons responded to mechanical (pinch or squeeze) and/or thermal (water bath or waterjet greater than 44 degrees C) noxious stimuli with a suppression or a marked and sustained decrease in activity. 5. All of the nociceptive neurons responded to intense transcutaneous electrical stimulation with one or several components of activation or inhibition. According to their latencies, three types of components were distinguished: early, intermediate, and late components. We estimate that the early and the intermediate components would be triggered by the activity of peripheral fibers in the 6- to 20-m/s range and therefore could be in the A delta fibers range, whereas the late component would be triggered by fibers in the 0.5- to 1-m/s range and therefore could be in the C fibers range. 6. The neurons excited or inhibited by noxious stimuli were not homogeneously distributed in and around the Ce.(ABSTRACT TRUNCATED AT 400 WORDS)

Acoustic Stimulation↗