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Biomedical subjects

G Fiedler

Publications and source records attributed to G Fiedler.

25 records · Page 2Linked to original sources
PubMed1980

Transfection of protoplasts from Streptomyces lividans 66 with actinophage SH10 DNA.

The slightly modified procedure for the transformation of protoplasts of S. coelicolor A3 (2) with SCP2 plasmid DNA and polyethylenglycol (PEG) (Bibb et al., 1978) was extended to infection of protoplasts of S. lividans 66 with actinophage SH10 DNA (Klaus et al., 1979). Maximal yield of transfected protoplasts was obtained at 20% PEG, 3 MM sodium-citrate and 150 mM NaCl final concentrations. The efficiency of transfection was determined to be about 2 x 10(-8) to 2 x 10(-7). The average value of competent protoplasts was about 1-2 x 10(-4) of regenerating protoplasts. In comparison with outgrowing spores infected with phage particles the average burst size of transfected protoplasts was reduced from 100 to 10 pfu/infected cell, the latent period prolonged from 45 min to 120 min and the rise period was not affected.

Bacteriophages↗
PubMed1977

[Therapy of uremic pericarditis].

An uraemic pericarditis can appear as complication of a haemodialysis in different stages. At the beginning an uraemic pericarditis is still to be referred to the uncompensated retention or to adaptation processes in the cellular mineral metabolism. An uraemic pericarditis in the later course of the dialysis nearly regularly is connected with an underdialysis syndrome which can be evoked by fistula or shunt complications or by infections of different genesis (sepsis, hepatitis, virus infections). - It is reported on own experiences in the dialytic treatment of uraemic pericarditis. In the period from 1970 to 1976 among 83 patients who were in chronic dialytic treatment an uraemic pericarditis was observed in 21 cases. In 3 patients developed haemorrhagic effusions with tamponade. The longest survival time of a patient with uraemic pericarditis and acute cardiac arrest in the early stage of the dialytic treatment is up to now 5 1/2 years after transplantation of a kidney of a corpse. - The possibilities of an operative exonneration of the heart from pericarditic changes are discussed.

Chronic Disease↗