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Biomedical subjects

G Fontan

Publications and source records attributed to G Fontan.

29 records · Page 2Linked to original sources
PubMed1978

A simple and reproducible method to evaluate granulocyte adherence.

A simple method was devised to measure granulocyte adherence in whole blood. Columns of glass beads (4.5 mm diameter) in disposable plastic syringes were used. The assay showed great reproducibility when done in triplicate, the day to day variations in a given individual being minimal. Previous incubation of the blood with different ethanol concentrations diminished granulocyte adherence. The assay is easy to perform and does not require special equipment.

Cell Adhesion↗
PubMed1978

Neutrophil chemotaxis and serum chemotactic activity in systemic lupus erythematosus.

Neutrophil chemotaxis, random motility, serum chemotactic activity derived from complement activation by classical or alternative pathways, and the presence of serum inhibitors of chemotaxis were all studied in 24 patients affected by Systemic Lupus Erythematosus (SLE) and in an equal number of healthy control subjects. Statistical comparison between patients and controls indicated lower chemotactic activity in patient's serum when activated by the classical pathway, and the presence in some SLE patients of a heat-labile inhibitor of the chemoattractants. Low "classical pathway" chemotactic indexes were correlated with low C4 values, active nephritis and recurrent infections. The presence of heat-labile inhibitor was correlated with low values of C3. Our data suggest that defective neutrophil chemotaxis could be one of the mechanisms contributing to the high incidence of infections suffered by SLE patients. The importance of conducting separate studies on cell movement and on generation of serum chemotactic activities by classical and alternative pathways in SLE patients is discussed.

Adolescent↗
PubMed1977

[Complement system in disseminated lupus erythematosus].

The levels of C3, C4 and CH50 in patients with lupus erythematosus disseminatus (LED) were lower than in the controls. However, although in many patients these levels were below the normal values, there was no statistically significant difference between the two group. The levels are reduced during the acute phases and in some patients they remain high. On the whole, C3 was lower in patients with nephropathy (p less than 0,025) than in patients presenting with clinical activity (p less than 0,02). The positive relationship existing between C3 and C4 (r = 0,641, p less than 0,01) suggests activation of the complement system in LED via the classic route. In cases with concurrent lupus nephropathy a relationship between C3 and C3PA was seen, suggesting that in this case the alternative route is involved.

Adolescent↗
PubMed1976

Defective neutrophil chemotaxis and hyperimmunoglobulinemia E-a reversible defect?

An eleven-month-old boy is presented with chronic atopic dermatitis and recurrent infections of the skin and respiratory tract, including subcutaneous abscesses. Immunological studies disclosed a neutrophil chemotactic defect, blood eosinophilia and serum hyper IgE. The clinical and analytical data are similar to those of patients previously dermatitis reversed the chemotatic defect, the blood eosinophilia and the clinical symptoms.

Chemotaxis↗
PubMed1976

Defective neutrophil motility in hypovitaminosis D rickets.

The chemotactic activity and random motility of neutrophils, was studied in 38 patients with hypovitaminosis D rickets, and compared with 29 healthy controls of matched age. The chemotactic activity derived from the activated rickets serum as well as the amounts of the complement components C4, C3 and C5 was normal, but the cell motility was clearly defective (p less than 0.001). A possible relationship between defective neutrophil movement and the recurrent infections seen in these patients is suggested. The possible mechanisms responsible for the defect could be the alteration in Ca/P metabolism or a defective action of the vitamin D on the neutrophils.

Cell Movement↗