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Biomedical subjects

G G De Lange

Publications and source records attributed to G G De Lange.

7 recordsLinked to original sources
PubMed1993

HTLV-II seroprevalence in pygmies across Africa since 1970.

HTLV-II-specific antibodies, with patterns similar to those in the Americas, were present in sera collected about 1970 from Bambuti pygmies in Zaire (14/102; 14%) and from pygmies in Cameroon (5/214; 2.3%), and were more prevalent than HTLV-I. In the Central African Republic, 504 pygmies were HTLV negative. After finding of 4 HTLV-II seropositives among 12 Bambuti pygmies sampled in 1991, this established that HTLV-II or a related retrovirus is present as an ancient endemic in some, but not all, insulated groups of African pygmies, similar to the HTLV-II distribution in Amerindian populations. The endemic among the oldest inhabitants of central Africa, and the occasional and scattered occurrence of apparent HTLV-II among predominant HTLV-I in other Africans, fit well with an ancient African virus and not with importation from the New World. Theories on the origin and evolution of the primate T-lymphotropic viruses (PTLVs) should take into account the longstanding presence of HTLV-II-type viruses in both the Old and New World. Present serology suggests identity of the African viruses with HTLV-II, but their assignment to a new HTLV type is open should genetic analysis show strong divergence from American HTLV-II. Clinical expression, if any, remains to be studied.

Blotting, Western↗
PubMed1991

The first monoclonal anti-G1m(x) antibody: production and usefulness in haemagglutination-inhibition assay.

Murine monoclonal anti-human Ig allotype antibodies have been described for a limited number of specificities. Now the first monoclonal anti-G1m(x) antibody has been produced by fusion of lymph gland cells of a mouse injected with G1m(zax)-positive myeloma proteins with cells of the myeloma cell line SP2/0. Several limiting dilutions resulted in one antibody-producing hybridoma, clone 3A12. The specificity of monoclonal antibody (mAb) 3A12 in the direct haemagglutination assay, as well as in the haemagglutination-inhibition (HAI) assay, is described. Neither cross-reactivity nor nonspecific inhibition with G1m(x)-negative Ig was seen. Typing for the G1m(x) allotype with mAb 3A12 gave results which are identical to those obtained with the conventional human antisera. With the production of this mAb, it now is possible to discriminate between the four main G1m alleles not only by HAI assay but also by other assay systems, of which the preliminary results are promising.

Animals↗
PubMed1989

Gm haplotypes in inflammatory demyelinating polyneuropathies.

The possible association of Gm haplotypes with inflammatory neuropathies has been studied in 59 patients with Guillain-Barré syndrome and 55 patients with chronic inflammatory demyelinating polyradiculoneuropathy. The frequency of Gm haplotype 1,2,17;21 (or z,a,x;g) was significantly raised in the patients with Guillain-Barré syndrome. These findings provide further evidence for an association of chromosome 14 genes with inflammatory neuropathies.

Demyelinating Diseases↗
PubMed1988

Linkage studies in facioscapulohumeral muscular dystrophy.

Possible linkage between the locus for autosomal dominant facioscapulo-humeral muscular dystrophy and the locus for the constant region of the heavy chains of the IgG immunoglobulins (Gm) was tested in 1 kindred (23 affected and 18 unaffected sibs) using the polymorphic DNA probe D14S1, which is known to be closely linked with Gm. No linkage between the loci for the disease and the probe was found, and the lod scores suggested that the locus for facioscapulohumeral muscular dystrophy is not situated on the distal part of the long arm of chromosome 14.

Chromosome Mapping↗
PubMed1987

Genes on chromosome 14q and their role in the pathogenesis of HLA-B27 associated diseases.

Genetic factors other than HLA-B27 may play a role in the pathogenesis of ankylosing spondylitis (AS), acute anterior uveitis (AAU) and Reiter's syndrome (RS). Studies by Brewerton et al. and Kijlstra et al. showed associations between the MZ phenotype of alpha 1-antitrypsin and the Gm phenotype zafngb of IgG in patients with AAU, who developed AS. The loci for alpha 1-antitrypsin (PI) and Gm allotypes (IGH) are situated on the tip of the long arm of chromosome 14. In the present study we tried to clarify and extend the above studies. In 41 B27+ AAU patients with AS the alpha 1-antitrypsin and Gm phenotype and allotype frequencies were not statistically different from those in B27+ AS patients developing AAU and in B27+ AAU patients without AS, in B27+ AS patients without AAU, B27+ patients with Reiter's syndrome, B27+ patients with low back pain, B27- AAU patients and normal controls. It is therefore unlikely that genes on the tip of chromosome 14 play a role in the pathogenesis of B27 associated diseases. A hypothesis was formed suggesting that a bacterial-derived modifying factor may replace the position of beta 2 microglobulin in the HLA-B27 molecule resulting in an impaired cytotoxic T cell reactivity.

Alleles↗
PubMedDate not supplied

IGHG1 phenotypes in three population samples from Italy (Albanians, Calabrians, northern Italians): a critical revision.

This paper presents data on the distribution of IGHG1 phenotypes in three populations living in Italy (Albanians, Calabrians and Northern Italians). IGHG1 typing for the allotypes z, x, a, f has been performed by using standard antisera provided by the Central Laboratory of the Netherlands Red Cross Blood Transfusion Service (Amsterdam). The results are compared with those obtained for the same populations using alloantisera from polytransfused subjects and Behringwerke antisera. It is shown that the results obtained by using the different antisera are not in agreement. Moreover, standard antisera reveal that the IGHG1 za haplotype is more frequent in Albanians than in Northern Italians.

Adolescent↗