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Biomedical subjects

G G Kuznetsova

Publications and source records attributed to G G Kuznetsova.

18 recordsLinked to original sources
PubMed1989

[A micromethod for the rapid detection of proteolytic activity of microorganisms].

The author has developed a micromodification of the rapid photoemulsion method for the detection of the microorganism proteolytic activity. The test is carried out on polystyrene plates, meat-peptone broth is used as the suspension fluid. Rectangular film strips are vertically placed into the wells, thus preventing false-negative reactions possible in case of an erroneous horizontal position of the film with the emulsion layer turned upwards if square or round film fragments are used. The proteolytic activities of 120 microorganism strains (95 of these hydrolyze gelatin) have been examined by the micromethod and the routine test with gelatin. The suggested test is 2-4-fold more rapid than the routine one used in investigations of the cultures slowly hydrolyzing gelatin. The fact that the test is carried out on the plates considerably reduces the nutrient medium and the number of laboratory glassware and helps obtain more accurate results.

Bacteria↗
PubMedDate not supplied

[Biochemical properties of enterobacteria on polystyrene plates].

The authors studied 84 strains of enterobacteria. The reliability of the results of the biochemical testing obtained by the micromethod with the use of polystyrene plates was examined comparatively to the data obtained by the classical method and API microsystems manufactured in France. Of the 84 strains of enterobacteria, 36 belonged to Proteus, 24 to Enterobacter, 8 to Klebsiella, 5 to Citrobacter, and 11 to E. coli. Of the strains tested, 50 were isolated from the intestine of children and adults, 30 from the residual microflora of foods, and 4 were museum strains. Study according to 23 tests on the conventional media with consideration of the results after 18 to 48 hours demonstrated the coincidence of the data, obtained by the methods used, for most strains. This circumstance permits recommending the micromethod for testing the biochemical properties of enterobacteria on polystyrene plates for broad-scale use.

Bacteriological Techniques↗
PubMedDate not supplied

[Effect of a derivative of GABA, phenibut, on behavior and the activity of visual cortex neurons of the rabbit during elaboration of a defensive reflex and internal inhibition].

In experiments on alert non-immobilized rabbits the effect of subcutaneous administration of the GABA-derivate--phenibut on behaviour, slow potentials and impulse activity of neurones of the visual cortex was studied during elaboration of a defensive reflex to light flashes and of conditioned inhibition. During the action of phenibut late negative-positive components of the evoked potentials to flashes, corresponding inhibitory pauses and postinhibitory activation gradually increased; then stable predominance of slow high-amplitude potential oscillations and corresponding neuronal group bursts appeared, separated by inhibitory pauses and in intersignal periods. Reinforcing stimulus, as before phenibut administration, lowered the amplitudes of slow potential oscillations and weakened inhibitory pauses in neuronal impulse activity. Dynamics of movements in response to the stimuli was of a phasic character. 3 hours after phenibut administration the discrimination of reinforced and inhibitory light flashes has distinctly improved. The obtained results confirm the initial concept of the significant role of the GABA-ergic inhibitory system in the process of elaboration of internal inhibition.

Animals↗
PubMedDate not supplied

[Effect of a liquid acidophilic mixture on the intestinal microbiocenosis of infant patients in the 1st months of life].

The effect of a liquid acidophilic mixture on intestinal biocenosis was studied in 10 premature infants aged 19 days to 1 1/2 months (Group I) with focal inflammation and neurological disorders after hypoxia in the perinatal period, and in 11 children aged 19 days to 3 1/2 months (Group II) with sepsis and intestinal diseases of staphylococcal, proteus and obscure etiology. Before application of the mixture, all the children demonstrated microbiocenosis disorders which were more remarkable in Group II. The liquid acidophilic mixture was shown to have a correcting action as regards lactobacilli, and the quantitative and qualitative composition of intestinal bacteria. The correcting effect exhibited by the mixture was found inadequate to neutralize an adverse effect of the infectious process and intense antibiotic therapy and chemotherapy on intestinal microbiocenosis in children of the first months of life suffering from sepsis and intestinal diseases, provided the mixture was used for 11-20 days.

Asphyxia Neonatorum↗