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Biomedical subjects

G G MacPherson

Publications and source records attributed to G G MacPherson.

44 records · Page 3Linked to original sources
PubMed1977

The traffic of mononuclear phagocytes through renal allografts in sheep.

Five to ten per cent of the cells in lymph draining renal allografts in sheep have the morphology of mononuclear phagocytes (MNP). These cells have been examined by light and electron microscopy and their functional characteristics investigated. The vast majority of MNP were of recipient origin. Their traffic increased from about 10(6)/hr immediately after grafting to between 2 and 4 X 10(7)/hr just prior to rejection. During the 1st 2 to 3 days, the majority of MNP had the morphology of monocytes, but the proportion of mature macrophages increased steadily to 30%. During the later stages, many of these large macrophages showed features of activation in terms of their spreading and phagocytic capacities. No NDA synthesis was observed in MNP until the latest stages, when a small proportion (less than 1%) of MNP labelled in vitro. Short-term cultures of lymph cells from kidneys undergoing rejection gave rise to numerous giant cells. It is suggested that there is continual traffic of MNP through the grafted kidney and that a proportion of MNP differentiate within the kidney and show features of activation.

Animals↗
PubMed1977

Erythropoietin in renal and hepatic lymph of conscious ewes.

Spontaneously flowing fistulae were established in the efferent lymphatics of popliteal, prescapular and prefemoral nodes and lumbar trunk or in the afferent lymphatics draining the kidney and liver of sheep. Lymph was collected from these sites over various time intervals and assayed for erythropoietin (Ep) content. The objective of the study was to establish the anatomic site(s) of Ep production. Normal lymph did not contain detectable titers of Ep, nor did renal lymph or blood plasma from a sheep systematically treated with cobaltous chloride. Renal lymph did contain measurable levels of Ep following renal artery constriction, unilateral hydronephrosis or phenylhydrazine-induced hemolytic anemia. Phenylhydrazine treatment also produced elevated Ep levels in lymph from the liver but not in lymph efferent from either popliteal or prescapular nodes. These results indicate that Ep is generated primarily in the kidney and that the liver may be an extrarenal source of the hormone. The surgical techniques used in this study offer distinct advantages in examining the composition and physiology of lymph in sheep.

Anemia, Hemolytic↗