A modified version of quantitating repetitive sequences in genomic DNA was developed to allow comparisons for numerous individual genomes and simultaneous analysis of several sequences in each DNA specimen. The relative genomic content of ribosomal repeats (rDNA) was estimated for 75 individuals, including 33 healthy donors (HD) and 42 schizophrenic patients (SP). The rDNA copy number in HD was 427 +/- 18 (mean SE) per diploid nucleus, ranging 250-600. In SP, the rDNA copy number was 494 +/- 15 and ranged 280-670, being significantly higher than in HD. The two samples did not differ in contents of sequences hybridizing with probes directed to a subfraction of human satellite III or to the histone genes. Cytogenetic analysis (silver staining of metaphase chromosomes) showed that the content of active rRNA genes in nucleolus organizer regions is higher in SP compared with HD. The possible causes of the elevated rRNA gene dosage in SP were considered. The method employed was proposed for studying the polymorphism for genomic content of various repeats in higher organisms, including humans.

Adolescent↗

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[Hemodynamics and rheologic properties of the blood in meningococcemia associated with meningitis and complicated by septic shock and intracranial hypertension].

Central hemodynamic parameters characterizing cardiac contractility and peripheral resistive vascular tone, as well as blood aggregation ability, its structural and dynamic viscosity have been assessed by dynamic (disease duration up to 15 days) and complex studies in 28 patients with septic shock of meningococcal etiology accompanied by signs of meningitis. All the patients revealed signs of intracranial hypertension caused by the onset of acute hydrocephaly or brain edema. It has been demonstrated that on entry all the patients were characterized by the combination of low cardiac output syndrome and second degree high or low blood viscosity. In addition to endotoxemia, acute intracranial hypertension that enhances the afterload and blood viscosity abnormalities are involved in the development of hemodynamic disturbances. Over the whole observation period elevated intracranial pressure had a certain effect on central hemodynamics, changing according to the mechanism similar to that observed in Cushing's syndrome. In addition, central hemodynamics and blood viscosity are directly or indirectly (via affected CNS) influenced by Herpes infection developing on days 2 to 5 of the disease.

Adolescent↗

PubMedDate not supplied

[A method of hemosorption in infectious diseases with the symptoms of hypocoagulation, intracranial hypertension and brain edema].

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Adolescent↗