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Biomedical subjects

G Garnier

Publications and source records attributed to G Garnier.

At least 91 records · Page 5Linked to original sources
PubMed1989

Heterogeneous nature of human complement factor B: an electrophoretic approach for the analysis of its oligosaccharide chain structure and its physiological breakdown products.

Factor B is a glycoprotein which plays an essential role in the alternative pathway of complement activation. It carries the proteolytic activity of the convertases, and its physiological breakdown products Ba and Bb have some effects on the cells of the immune system. Human factor B exhibits a microheterogeneity and five isoforms are present in serum. The nature and origin of the microheterogeneity was investigated by using electrophoretic techniques. Treatments of factor B with neuraminidase and glycopeptidase F show that this microheterogeneity is mainly due to differences in its sialic acid content, varying from seven to eleven residues per molecule, and resulting in different oligosaccharide structures. However, deglycosylated factor B reveals a residual, nonallotypic variation in the Bb region of the polypeptide backbone. We confirm the presence of four asparagine-linked oligosaccharide chains of the complex type in native factor B, two of which are located in the Ba fragment, and the two others in the Bb fragment. The prevalent isoform of the native protein carries two sialic acid residues per oligosaccharide chain. Biosynthesis experiments show that the microheterogeneity of secreted factor B from HepG2 cells is acquired during the processing of its glycans. However, in vitro-secreted factor B is more heterogeneous than the serum protein. We propose a structural model for the microheterogeneity of the native protein and its physiological fragments. We discuss as well the feasibility of electrophoretic techniques to deal with microheterogeneity analysis.

Amidohydrolases↗
PubMed1989

[Value of repeated thoracic scanning in the surveillance of operated bronchial cancers].

The authors report three cases of endothoracic tumour recurrence in patients who have been operated on for primary lung cancer revealed by CT scanning, carried out systematically in the respiratory unit to a pre-established protocol. There were no other clinical, fibre-optic, or radiological signs of these recurrences before their discovery using CT scanning. The authors discuss the value of thoracic scanning in the systematic follow up of operated cases of bronchial cancer.

Adenocarcinoma, Bronchiolo-Alveolar↗
PubMed1988

Molecular basis for the microheterogeneity of human complement factor B.

The involvement of sialic acids in the microheterogeneity of human complement factor B was investigated. Desialylation kinetics revealed all the charge intermediates from a complex native to a homogeneous form. The relation between this heterogeneity and posttranslational events was explored in cultured hepatoma cells. Intracellular factor B exhibited the same isoelectric focusing pattern as the desialylated purified protein, whereas a highly heterogeneous form was secreted. In contrast, when N-glycosylation was prevented by tunicamycin, both intracellular and secreted forms focused like intracellular factor B from control cultures. These data lead to the conclusion that the microheterogeneity of human factor B results from different degrees of sialylation of its N-glycans.

Carcinoma, Hepatocellular↗
PubMed1987

[Prognostic factors of small cell bronchial carcinomas].

Prognostic factors in small cell lung carcinoma have a great importance with regard to the interpretation of treatment results. The main prognostic factors are the extent of the disease, performance status, age and sex. Other factors are discussed.

Age Factors↗
PubMed1986

[Bronchial involvement disclosing Wegener's granulomatosis].

The authors report a case of Wegener's granulomatosis, whose initial manifestation was an isolated endobronchial stenosis. In connection with this observation, it calls to mind, within the scope of the pulmonary granulomas (in view of such a rare clinical presentation) the difficulties of differential diagnosis, in particular with bronchocentric granulomatosis, and stresses the need for pulmonary histological examination to open lung biopsy.

Biopsy↗
PubMed1986

[2 cases of menstrual pneumothorax].

The authors report two new cases of menstrual pneumothorax. In both cases the lesions of endometriosis seemed to dominate the pathogenesis. After a rapid review of the literature to elucidate the main clinical characteristics of this curious syndrome, the authors stress the advantage of a pleuroscopic examination in women with the syndrome.

Adult↗
PubMed1984

[Spontaneous regression of a bronchial epidermoid cancer].

A spontaneous regression of epidermoid carcinoma of the bronchus is reported here for only the third time. This report concerns a 57 year old patient suffering from chronic airflow obstruction who had twice required artificial ventilation. Fibroptic bronchoscopy was performed on account of hilar asymetry and a small tumour on the carina of the middle lobe was discovered. The biopsy confirmed the existence of a moderately differentiated invasive epidermoid bronchial carcinoma. An examination performed four years later failed to reveal this tumour. Immunological studies showed only a notable rise in NK cells.

Carcinoma, Bronchogenic↗