PubMed Health⌕ Search

Biomedical subjects

G Geier

Publications and source records attributed to G Geier.

49 records · Page 3Linked to original sources
PubMed1975

[Regional different cell-proliferation in placetones (materno-fetal circulation-units) of mature human placenta (author's transl)].

The examinations presented are based on earlier histological, histochemical, ultra-histochemical and biochemical studies about regional differences of placental villi in the center and the periphery of the placentons (materno-fetal circulation-units) of mature human placentas. In this study the question was followed up whether the morphological "immature" imposing villi lying in the centers of the circulation-units are immature like the villi of placentas of earlier stages of pregnancy also referring their proliferation. Autoradiograms were made from specimens of placentas of 38.-41. weak of uncomplicated pregnancies. The degree of nuclear labeling was higher in the villi situated in the center of the placentons. By these findings the centers of the placentons are interpreted to be not areas of lower metabolism - possibly conditioned by maternal blood flow - but regions of growth and regeneration. The results comply well with our up to date conception of formation and growth of the placentons.

Autoradiography↗
PubMedDate not supplied

Two-dimensional gel electrophoresis of Caenorhabditis elegans homogenates and identification of protein spots by microsequencing.

Employing isoelectric focusing on immobilized pH gradients followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) we have obtained a map of C. elegans proteins, from a mixed culture containing all developmental stages, presenting over 2000 spots within the window of isoelectric points (pI) 3.5-9 and a molecular mass of 10-200 kDa. Edman microsequencing yielded successful results in 12 out of 24 analyzed spots. All but one of the N-terminal sequences retrieved C. elegans sequences in cosmid and/or expressed sequence tag clones. Structurally related protein sequences found in data banks included enzymes in energy metabolism (cytochrome oxydase, ATP synthase, enolase), a fatty acid-binding protein, a translationally controlled tumor protein, an unknown C. elegans protein, an acidic ribosomal protein, a titin-like protein, a G-protein beta chain, cyclophilin, and cathepsin D. Experimental determination of N-termini allowed us to define sites of signal cleavage providing further information on the physiological role of the newly found C. elegans proteins. This report demonstrates the possibility of two-dimensional gel electrophoresis and Edman microsequencing in the elucidation of C. elegans proteome.

Amino Acid Sequence↗
PubMedDate not supplied

Cytologic findings in vaginal smears from patients under treatment with cyclophosphamide.

A report is given on the vaginal smears of 55 patients with ovarian carcinomas who were treated with cyclophosphamide either alone or in combination with other therapies. The changes occurring under the treatment are demonstrated and compared with the findings before therapy. A shift to lesser maturation depending upon drug dose and the appearance of the so-called "polychromatic and polymorphous near type" are described. This smear type was found in about 60 per cent of all cases and was reversible after cessation of the therapy. The characteristics of the "polychromatic and polymorphous smear type" are presented.

Aged↗