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Biomedical subjects

G Gerna

Publications and source records attributed to G Gerna.

At least 199 records · Page 11Linked to original sources
PubMed1975

[The immunoperoxidase technic in the rapid identification of human cytomegalovirus on primary isolation].

Immunoperoxidase technique has been applied to the rapid identification of human cytomegalovirus (CMV) strains on primary isolation. Negative and positive results have been constantly confirmed by indirect fluorescent antibody technique (IFA) and by electron microscopy. Using either direct or indirect method it was observed that problems of nonspecific staining of uninfected cells were encountered mostly with direct method, probably because of the lower working dilution of the conjugate (1:20) as compared with higher conjugate dilution used for indirect method (1:150). However, nonspecific cytoplasmic stain of Golgi area of infected cells was observed also with indirect method. Pathogenesis of nonspecific labelling of this area is discussed. Application of the immunoperoxidase technique directly into tubes on primary isolation allows to reduce identification time in comparison with IFA technique and is less cumbersome and time-consuming than complement fixation and neutralization tests.

Antibody Specificity↗
PubMed1975

Nonvirionic inhibitor of DNA synthesis in human embryo fibroblast cell cultures infected with rubella virus.

Human embryo fibroblast cell cultures (RU-I) infected with virulent and attentuated strains of rubella virus of treated with a cell extract form infected and from control cultures have been studies in respect of their mitotic activity by evaluating the (3-H) thymidine uptake by autoradiography. The results show the presence in infected cell cultures of a nonvirionic inhibitor of DNA synthesis, which is detectable in control cell cultures too, though to a lesser degree. The relation of this inhibitor to the chalone system is so far unclear. No effect of the extracts on human chromosome pattern was observed. Attempts of biochemical characterization by fractionation of 14-C-labeled extracts on polyacrylamide gel followed by autoradiography were unsuccessful.

Cells, Cultured↗
PubMed1975

[1:7 ratio in the distribution of adw and ayw subtypes in a population of HBs Ag-positive hepatitis patients in northern Italy].

The subtypes of HBsAg have been determined in 41 cases of acute type B hepatitis by counterelectrophoresis (CEP) using mono-specific D (anti-d), Y (anti-y), W (anti-w) and R (anti-r) antisera following Dr. Holland's technique. In 36 cases (87.8 percent) the subtype was ayw and in 5 (12.3 per cent) adw. Neither r subtypes nor simultaneous presence in the same patient of both d and y subtypes were detected. The subtype distribution in Itayl is closely related to the observed distribution of d/y subtypes in the Mediterranean countries, except for Spain, showing a pattern similar to that of Central and Northern Europe countries.

Blood Group Antigens↗