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Biomedical subjects

G H Counotte

Publications and source records attributed to G H Counotte.

14 recordsLinked to original sources
PubMed1992

[The effect of feeding mangel-wurzels on blood beta-hydroxybutyrate level of milk-producing cows].

The effect of feeding dairy cattle mangel-wurzels on the serum increased concentration of beta-hydroxybutyrate was investigated in serum is normally indicative of an increased catabolism of fats in the liver as a result of an energy shortage. Feeding cattle large amounts of mangel-wurzels can cause the serum beta-hydroxybutyrate concentration to increase above normal reference values, thus seemingly indicating that there is a (sub)clinical acetonaemia. In fact the increased concentration of beta-hydroxybutyrate is probably the result of an increased production of butyrate in the rumen.

3-Hydroxybutyric Acid↗
PubMed1992

Haematological and clinicochemical blood profiles in slaughter pigs.

In a cohort study, 40 pig finishing herds were selected: twenty pig herds with a low and twenty pig herds with a high prevalence of several pathological lesions recorded at slaughter in a six-month period before the start of the study. Blood samples were taken from 20 pigs per herd at the end of the finishing period to investigate haematological and clinicochemical profiles. There was only a significant difference in serum albumin concentration between the low and high lesion prevalence groups. There were distinct differences in blood profiles between pig herds, but also between batches of pigs within a herd, housed in different compartments. Differences between castrated males and gilts were also demonstrated with respect to mean values of the blood variables haemoglobin, iron, copper, beta-globulin, eosinophils and segmented neutrophils. However, the differences were not of a biologically important magnitude. The mean values of the blood variables pepsinogen and lymphocytes differed significantly between pig herds from the two participating integration groups. Pigs with a higher albumin concentration, a lower gamma-globulin concentration, a higher copper concentration and a higher creatine kinase activity in serum showed a higher daily weight gain.

Abattoirs↗
PubMed1990

[Triclabendazole (Fasinex) residue in milk: determination and excretion kinetics].

The determination of triclabendazole (Fasinex) in milk is described. The excretion of triclabendazole and its two main metabolites was measured under field conditions in twenty-five cows. Triclabendazole was administered to cows in a dosage of approximately 12 mg kg-1. The maximum concentration of the main metabolites of triclabendazole (triclabendzole sulphone) measured in milk was 1.415 mg/l. Metabolites could be detected in milk during a ten-day-period. Only 1.5% (maximum) of the triclabendazole administered was eliminated as triclabendazole sulphone by way of milk.

Animals↗
PubMed1989

[A study of the usefulness of a metabolic profile test in determining the causes of herd problems on dairy farms in The Netherlands].

A metabolic profile test (MPT), consisting of the following parameters of the blood: glucose, urea, beta-hydroxybutyrate, copper, magnesium, calcium, non-esterified fatty acids, bilirubin, aspartate amino transferase, whole protein and the protein spectrum of the serum, was performed in twenty-two normal herds (control herds) in the Netherlands. Reference levels were estimated for the above parameters. The study showed that this MPT did not discriminate between normal herds and herds showing production problems or an increased incidence of metabolic diseases. Statistically significant differences between the three laboratories taking part in these investigations were not observed.

Animals↗
PubMed1989

Relation between selenium content and glutathione-peroxidase activity in blood of cattle.

To identify a simple method for assessing the selenium demand in cattle, the relationship between selenium content in whole blood and the concentration of the selenium containing enzyme glutathione peroxidase (EC 1.11.1.9; GSH-Px) in red blood cells was studied. On six farms with suspected low soil selenium content, blood samples were collected from groups of calves, yearlings and adults at the end of the housing period and of the grazing period. The data obtained showed a highly significant correlation between the parameters mentioned: GSH-Px (U/g Hb) = 3.261 * Se (micrograms/kg) - 40.553. In growing animals there was a decline in Se supply with age, followed by a gradual recovery in heifers. This was most pronounced on some sandy soils and on peat soil. Seasonal effects could not be demonstrated.

Age Factors↗
PubMed1988

[Effects of the addition of increased nitrates to the drinking water of fattening pigs and weaned piglets].

Three experimental studies were done on the effects of increased concentrations of nitrate in the drinking water of weaned piglets and flattening pigs throughout the weaning and fattening periods respectively on the experimental piggery in Raalte for pigs in the northern and eastern Netherlands. To begin with, prospective studies were done in three times four individually housed experimental animals which were given 100, 200 and 500 mg of nitrate per litre of drinking water respectively throughout the fattening period. The results obtained were compared with the findings in twenty controls. When the weaned piglets were studied, two groups of weaned piglets, each consisting of an odd hundred piglets, fifty per cent of which served as an experimental group, the other fifty per cent serving as a group of controls, were compared. The experimental group was given drinking water to which 220 mg of nitrate per litre were added. When the fattening pigs were studied, two groups, each consisting of seventy animals, were compared. Fifty per cent served as a control group and fifty per cent as an experimental group. The experimental groups were given drinking water to which 500 mg of nitrate per litre were added. The nitrate and nitrite levels of the drinking water supplied were measured at regular intervals. The studies in fattening pigs included the examination of blood samples for the concentrations of haemoglobin and methaemoglobin halfway and at the conclusion of the periods. The additional nitrate in the drinking water did not have any negative effect on the haemoglobin and methaemoglobin levels of the drinking water or on the results obtained in these studies. Studies on meat and organs were done in five controls and five experimental animals from the fattening pigs studied. Marked differences were not observed in any case. It is concluded that an increased concentration of nitrate in drinking water does not have any injurious effect on the health of and the results obtained in weaned piglets, provided the drinking water is of good quality in addition to having an increased nitrate level.

Animals↗
PubMed1988

[Selenium deficiency as a cause of secondary retention of the placenta in a herd of dairy cattle].

A farm is described, on which the placenta was retained in approximately forty per cent of the cows and on which selenium deficiency (0.03 +/- 0.010 micrograms/ml) and unduly reduced activity of glutathione peroxidase (GSH-Px) in the erythrocytes (65.2 +/- 26.4 u/g Hb) were observed. When 0.5 mg of Se per kg of dry matter of a mixture of concentrated feeds were added, the concentrations of Se and GSH-Px were restored to normal and the symptom consisting in retained placentae disappeared. This incident shows that selenium deficiency occurs in the Netherlands.

Animal Feed↗
PubMed1987

[Effectiveness of a PVC ear tag impregnated with the insecticide flucythrinate].

A PVC ear tag containing the synthetic pyrethroid flucythrinate was tested in seventeen herds including 218 heifers. There was a comparable control group of twelve herds including 157 heifers. The ear tags were more effective against sucking flies than they were against sticking flies. Varying with the species of the fly, the effectiveness ranged from 36.2 per cent to 95.7 per cent. The effectiveness also varied between the herds. The active constituent was still released within approximately 5 1/2 months after introduction.

Animals↗
PubMed1986

[Determination of progesterone levels in the milk of mares: a useful aid in the diagnosis of early pregnancy].

The concentration of progesterone in milk was determined in 327 mares to establish pregnancy in an early stage (sixteen days or more). The 5 ng/ml-level of progesterone as a threshold in the EIA-system used resulted in a very useful aid in the diagnosis of early pregnancy in mares. The results of testing were not in accordance with the confirmed reality in fourteen out of 327 mares (4.3 percent). The result of the test was false non-pregnant in five mares (1.5 percent) and false pregnant in nine mares (2.8 percent).

Animals↗
PubMed1986

Usefulness of measuring serum lysozyme activity in dogs with neoplastic disease.

Serum lysozyme activity (SLA) was measured in a turbidimetric assay with a microcentrifugal analyzer. In a control group of 53 healthy dogs of both sexes and ranging in age from 4 to 10 years, SLA had a mean value of 1.2 mg/l with a range (+/- 2 SD) of 0.6 - 1.8 mg/l. In 80 dogs with a variety of neoplastic diseases the histopathological diagnosis was compared with the SLA value. SLA value was increased in 83% of the cases with malignant tumors and in 29% of the cases with benign tumors. Proper clinical examination is essential in differentiating between neoplastic disease and some interfering diseases, e.g. chronic dermatitis, pyometra and chronic nephritis. Measuring of SLA in dogs may be helpful in screening those animals with suspected malignancies.

Adenoma↗
PubMed1979

An analysis of the buffer system in the rumen of dairy cattle.

A method is presented for the analysis of buffer systems in the rumen using the first derivation of titration curves. Bicarbonate and volatile fatty acids (VFA) are the main components of the buffering system in the rumen fluid of dairy cattle under widely different feeding conditions. Phosphate from saliva is of little importance as a buffer, but neutralizes acids produced in the rumen. After studying five cows during the peripartal period a spontaneous and transient increase in the concentrations of VFA and a soluble marker (PEG) as well as a drop in pH and in the bicarbonate concentrations not related to feeding was observed in two animals that were sampled several hours before parturition. The potential risk of provoking rumen disturbances upon feeding animals close to the time of parturition, when buffering capacity may be minimal, is stressed.

Animal Feed↗