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G H Perdew

Publications and source records attributed to G H Perdew.

58 records · Page 4Linked to original sources
PubMed1982

Biosynthesis of mammary glycoproteins. Structural characterization of lipid-linked glucosyloligosaccharides.

The lipid-linked glucosyloligosaccharide intermediates for the biosynthesis of asparagine-linked glycoproteins in the lactating bovine mammary tissue were isolated from incubations of microsomes of this tissue with labeled sugar-nucleotides. These were shown to have the general structure (Glc)1 -3(Man)9(GlcNAc)2. A structural characterization indicated that glucose residues in these oligosaccharides are linked as Glc alpha 1 leads to 3, Glc alpha 1 leads to 3Glc alpha 1 leads to 3, and Glc1 leads to 2Glc alpha 1 leads to 3Glc alpha 1 leads to 3, to the tetramannosyl branch of (Man)9(GlcNAc)2 portion previously characterized as Man alpha 1 leads to 2Man alpha 1 leads to 2Man alpha 1 leads to 3(Man alpha 1 leads to 2Man alpha 1 leads to 6[Man alpha 1 leads to 2Man alpha 1 leads to 3]-Man alpha 1 leads to 6)Man beta 1 leads to 4(3)GlcNAc beta 1 leads to 4(3)GlcNAc [Vijay, I. K., Perdew, G. H., and Lewis, D. E. (1980) J. Biol. Chem. 255, 11210-11220]. The lipid-linked (Man)9(GlcNAc)2 appears to be the initial acceptor of the first glucosyl residue. Even though incubations of the bovine mammary tissue in vitro synthesize multiple isomers of a number of mannosyloligosaccharides, the glucosyloligosaccharides are all monoisomeric.

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PubMed1980

Biosynthesis of mammary glycoproteins. Partial characterization of the sequence for the assembly of lipid-linked saccharides.

Incubation of a membrane preparation from the lactating bovine mammary gland with UDP-[3H]GlcNAc, GDP-[14C]Man, and UDP-[3H]Glc results in the biosynthesis of 15 lipid-linked saccharides that differ from one another by a monosaccharide unit. Pulse and chase kinetics indicate that these glycolipids are related to one another as precursor products for the biosynthesis of asparagine-linked glycoproteins of this tissue. [Man-14C]- and [Man-14C, GlcNAc-3H]saccharides were prepared from corresponding glycolipids by mild acid hydrolysis. Following extensive purification by paper and gel filtration chromatography, structural characterization was conducted on tri-, tetra-, penta-, and undecasaccharides via size determination on calibrated columns of Bio-Gel P-2 and P-4, compositional analysis, exo- and endoglycosidase digestions, methylation, Smith degradation, and acetolysis. These structures were identified as: Man beta 1 leads to 4(3)GlcNAc beta 1 leads to 4(3)Glc-NAc, Man alpha 1 leads to 3Man beta 1 leads to 4(3)GlcNAc beta 1 leads to 4(3)GlcNAc, Man alpha 1 leads to 3(Man alpha 1 leads to 6)Man beta 1 leads to 4(3)Glc NAc beta 1 leads to 4(3)Glc-NAc, and Man alpha 1 leads to 2 Man alpha 1 leads to 2Man alpha 1 leads to 3(Man alpha 1 leads to 2Man alpha 1 leads to 6[Man alpha 1 leads to 2Man alpha 1 leads to 3]Man alpha 1 leads to 6)Man beta 1 leads to 4(3)GlcNAc beta 1 leads to 4(3)GlcNAc.

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