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G H STOLLERMAN

Publications and source records attributed to G H STOLLERMAN.

At least 37 records · Page 2Linked to original sources
PubMed1959

Evaluation of the "long chain reaction" as a means for detecting type-specific antibody to group a streptococci in human sera.

Certain strains of Group A streptococci showed striking increase in chain length when grown in liquid media to which was added human sera that contained antibody to M protein of homologous type. This "long chain reaction" was shown to be a highly specific and sensitive biological test for human type-specific antibody and correlated closely with the classical bactericidal test. Patients infected with Type 12 or Type 3 Group A streptococci showed the appearance of anti-M antibody in their sera by both methods at similar intervals during convalescence. Of 217 sera studied in these patients the two tests showed agreement in all but 11 specimens. Of 99 patients who were bled serially following Type 12 or Type 3 infections, and whose sera were tested by both methods, there was close agreement, the bactericidal test being only slightly more sensitive. The advantages and limitations of this new biological test for human type-specific immunity are discussed.

Antibodies↗
PubMed1958

Accessory plasma factors involved in the bactericidal test for type-specific antibody to group A streptococci. I. A typical behavior of some human and rabbit bloods.

The bloods of two apparently healthy human beings, of 25 studied, failed to produce a strong bactericidal test for type-specific antibody to the M protein of group A streptococci under in vitro conditions wherein most human blood leukocytes rapidly phagocytize and destroy virulent organisms in the presence of anti-M antibody and accessory plasma factors. The defect in bactericidal activity of these two individuals is associated with the plasma rather than with the blood leukocytes. Leukocytes suspended in these atypical plasmas showed a characteristic delay in the rate of activation of phagocytosis. Although previously the bloods of laboratory animals (except monkeys) had been reported to be much less active than human blood in this system, occasional exceptions were encountered in rabbits in this study. Two rabbits were found whose bloods were as strongly bactericidal against streptococci, in the presence of type-specific antibody, as the blood of the average "normal" human being. The atypical behavior of some human and rabbit bloods in the bactericidal test may be explained by variations in accessory plasma factors that are as yet unidentified and that influence the rate of phagocytosis of virulent streptococci in vitro in the presence of type-specific antibody.

Animals↗
PubMed1957

Long chain formation by strains of group A streptococci in the presence of homologous antiserum: a type-specific reaction.

Strains of group A streptococci, types 30 and 12, were observed to grow in extremely long chains in liquid media to which homologous antiserum was added. Addition to the media of antisera to heterologous types of streptococci failed to produce long chain growth. The long chain effect was destroyed by absorption of the antiserum with organisms of homologous type but was unaffected by absorption with organisms of heterologous types. The reaction disappeared at concentrations of antisera smaller than 0.25 per cent and was independent of complement or other heat-labile serum factors. Addition of trypsin to the culture to remove M protein from cells prevented long chain formation. The long chain effect depended upon the constant presence of antibody to the media. In its absence, the organisms promptly reverted to short chain growth. The phenomenon appears to have general applicability to those strains rich in M protein with only an occasional strain not responding as described. Further studies are in progress to determine the cause of this atypical response. The applicability of this phenomenon in detecting type-specific antibody using indicator strains of a variety of streptococcal types is discussed.

Antibodies↗
PubMed1956

Rheumatic fever.

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Rheumatic Fever↗