Insect viruses: serological relationships.
Explore the source record for details and available documents.
Antigens, Viral↗
Biomedical subjects
G H Tignor
Publications and source records attributed to G H Tignor.
[Comparative studies of two alphavirus by macroplaque assays and by a fluorescent focus method in Vero cell lines (author's transl)].
Immune anti-Sindbis and anti-Chikungunya ascitic fluids have been titrated by the classic way of plaque reduction and by a quick fluorescent focus method using an indirect staining. The results are comparable, allowing to consider the use of this quick and inexpensive technic for counting-assays by neutralization.
Animals↗
Plaque assay for rabies serogroup viruses in vero cells.
Plaque formation in Vero cells has been induced with seven rabies serogroup viruses either cocultivated as infected BHK-21 or Aedes albopictus cells, or directly cultivated as infected mouse brain.
Aedes↗
Neutralization and hemagglutination-inhibition tests with Crimean hemorrhagic fever-Congo virus.
Explore the source record for details and available documents.
Animals↗
Immunopathologic aspects of infection with Lagos bat virus of the rabies serogroup.
Explore the source record for details and available documents.
Animals↗
Experimental infection of dogs and monkeys with two rabies serogroup viruses, Lagos bat and Mokola (IbAn 27377): clinical, serologic, virologic, and fluorescent-antibody studies.
Explore the source record for details and available documents.
Animals↗
Experimental infection of dogs and monkeys with two rabies serogroup viruses, Lagos bat and Mokola (IbAn 27377). Gross pathologic and histopathologic changes.
Explore the source record for details and available documents.
Animals↗
Vaccination and challenge of mice with viruses of the rabies serogroup.
Explore the source record for details and available documents.
Animals↗
Antibody responses in spider monkeys following single and double infections with group B arboviruses.
Explore the source record for details and available documents.
Animals↗
The yellow fever epidemic in Ethiopia, 1961-1962: retrospective serological evidence for concomitant Ebola or Ebola-like virus infection.
Explore the source record for details and available documents.
Child↗
The acetylcholine receptor as a cellular receptor for rabies virus.
Characterization of specific host cell receptors for enveloped viruses is a difficult problem because many enveloped viruses bind to a variety of substrates which are not obviously related to tissue tropisms in the intact host. Viruses with a limited cellular tropism in infected animals present useful models for studying the mechanisms by which virus attachment regulates the disease process. Rabies virus is a rhabdovirus which exhibits a marked neuronotropism in infected animals. Limited data suggest that spread occurs by transsynaptic transfer of virus. The results of recent experiments at Yale suggest that viral antigen is localized very soon after injection at neuromuscular junctions, the motor nerve endings on muscle tissue. On cultured muscle cells, similar co-localization with the acetylcholine receptor is seen both before and after virus multiplication. Pretreatment of these cells with some ligands of the acetylcholine receptor results in reduced viral infection. These findings suggest that a neurotransmitter receptor or a closely associated molecule may serve as a specific host cell receptor for rabies virus and thus may be responsible for the tissue tropism exhibited by this virus. In addition to clarifying aspects of rabies virus pathogenesis, these studies have broad implications regarding the mechanism by which other viruses or viral immunizations might mediate autoimmune diseases such as myasthenia gravis.
Adhesiveness↗
[A rapid assay of Sindbis virus infectivity by counting immunofluorescence foci in "Aedes albopictus" cell culture (author's transl)].
The Aedes albopictus cell line is susceptible to numerous arboviruses but the appearance of cytopathic effect is observed mostly with flavivirus. A method of rapid titration of Sindbis virus by counting immunofluorescent foci is described, using this cell line.
Aedes↗