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Biomedical subjects

G Haralambie

Publications and source records attributed to G Haralambie.

11 recordsLinked to original sources
PubMed1979

Biochemical changes in a 100 km run: proteins in serum and urine.

Eleven male subjects took part in a 100 km running competition. Alterations in the total plasma protein and in ten individual plasma protein concentrations in blood and urine were measured prior to the run, immediately after and after 1 day of recovery. Five individual proteins showed a 7-10%, and lysozyme a 40%, increase in the plasma after the run. On the contrary, the haptoglobin concentration fell to 40% of its pre-race level. None of these variations were correlated with the plasma volume change. The present data showed a moderate hemolysis, as evidenced by plasma lysozyme and hemoglobin-haptoglobin binding. The urinary excretion of plasma proteins was slightly increased, especially albumin and alpha1-acid-glycoprotein. The renal clearance of plasma proteins revealed that the 100 km run induced a moderate increase of glomerular permeability without any signficant change in the tubular reabsorption process.

Adult↗
PubMed1978

Changes in serum creatine kinase and hexose phosphate isomerase activity with exercise duration.

In 18 groups of trained healthy male subjects (n = 166; age 17--42 years) the influence of type and duration of long-lasting exercise on the changes of creatine kinase (CPK) and hexose phosphate isomerase (PHI) activity in serum was studied. Above 65--70% of maximal aerobic capacity, a serum enzyme activity increase proportional to the exercise time was found with a different slope in each of the three exercise types examined [a) bicycle ergometer; b) ski race; c) impact-type exertion]. Work duration of more than 300 min induced in the third type of exertion an accelerated increase in serum CPK. A third degree equation best describes the changes in serum CPK for nine of our groups and five others from the literature. To ascertain the possible influence of intravascular hemolysis, sera were mixed with autologous erythrocyte hemolysates and enzyme activity measured. The findings are discussed with reference to critical aspects of the analytical methods, possible causes of serum enzyme changes, and possible use of enzyme measurements in evaluating adaptation of the organism to prolonged exercise.

Adolescent↗
PubMed1978

Creatine kinase and hexose phosphate isomerase activity in skeletal muscles of healthy male adults.

Creatine kinase (CPK) and hexose phosphate isomerase (PHI) activities were measured in surgically obtained skeletal muscle samples of healthy adult men (n = 45). Median values of 4,200 for CPK and 275 for PHI (U/g wet weight, 37 degrees C) were found. Duplicate determinations on 7 samples divided and treated separately gave a very good reproducibility. There is a significant relationship between the activity of the two enzymes (r = +0.798, p less than 0.001). The mean activity levels were for both enzymes higher in m. vastus medialis quadricipitis and m. triceps brachii, c. long., than in vastus lateralis quadric. and in deltoideus. PHI frequency distribution was not of the normal type but probably bimodal, suggesting muscle types with lower and higher glycolytic rate in man.

Adolescent↗
PubMed1978

Further studies on creatine kinase activity in human skeletal muscle.

Creatine kinase (CK) was measured in muscle samples from 9 female and 9 male adult subjects using comparatively two procedures. The improved method, using N-acetylcysteine as activator gives results about 1.5 times higher than that with reduced glutathione. The significant correlation existing between activities of CK and hexosephosphate isomerase was confirmed using the improved method. There also exists a correlation between CK and enolase activity. In this respect, there are no differences between muscle samples from male and female subjects.

Adult↗
PubMed1978

Human skeletal muscle enolase and factors influencing its activity.

Enolase activity was determined in human skeletal muscle extracts using two procedures to measure the phosphoenolpyruvate formed. The results closely agreed, as did double determinations on bipartite muscle pieces. Activities in m. vastus lateralis quadricipitis did not differ significantly in adult males, females and 11- to 14-year-old girls. M. vastus medialis and deltoideus exhibited significantly higher activities than vastus lateralis. Endurance-trained athletes had lower enolase activities when compared to untrained subjects. The effects of changes in temperature, pH and K+ concentration in the medium were investigated and the possible physiological implications discussed.

Adolescent↗
PubMed1978

The effect of weight lifting exercise on heart rate and metabolism in experienced weight lifters.

Fifteen experienced weight lifters were investigated to examine the effects of weight exercise with variable intensity on heart rate and changes in arterial glucose, lactate and pyruvate, as well as serum total lipids, beta-lipoproteins, triglycerides, glycerol and free fatty acids. The highest value of heart rate were reached during repetitions (ca. 150 beats/min), during single maximal contraction only 120--130 beats/min. No important changes were found in lipid substrates or glucose, while lactate (x max: 4 mmol/l) and urivate (x max: 0.11 mmol/l) increases were moderate. This type of training may be useful in the clinical rehabilitation of the locomotor system but it lacks importance for preventive or rehabilitation therapy of the cardiovascular system.

Adult↗
PubMed1977

Fumarase activity in skeletal muscle of man.

Soluble extracts of human skeletal muscle have a fumarase activity of 31.2 +/- 7.27 (M. vastus lateralis quadricipitis) or 30.9 +/- 8.0 U/g wet weight at 37 degrees C (M. deltoideus). The distribution of activities in the 36 muscle samples studies is not gaussian. There is a significant correlation between the activities of citrate synthase and fumarase (r = + 0.881; p less than 0.001) in all investigated muscles, excepting M. vastus lateralis quadricipitis.

Adolescent↗
PubMed1976

Serum gamma-glutamyl transpeptidase and physical exercise.

The immediate and delayed influence of exercise of variable type, duration and intensity on the serum gamma-glutamyl transpeptidase activity (gamma-GT) has been examined in athletes and untrained persons. The possible effects of exercise-induced changes in other parameters (plasma free glutamate, serum triglyceride, haemoconcentration) on the measured postexercise serum gamma-GT have been discussed, partly on the basis of our own experimental data. It appears that neither exercise itself, nor any one of the above mentioned factors (excepting a slight, transient effect of haemoconcentration after brief, intensive exertion) have noticeable influence on serum gamma-GT. Determinations of this enzyme for diagnostic purposes are therefore likely to be used without regard to acute and chronic exercise history in man.

Adolescent↗