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Biomedical subjects

G He

Publications and source records attributed to G He.

At least 91 records · Page 5Linked to original sources
PubMed1998

Effect of vasopressin antagonism on structure and mechanics of small arteries and vascular expression of endothelin-1 in deoxycorticosterone acetate salt hypertensive rats.

The structural and mechanical properties of small arteries are altered in rat models of hypertension. The precise role of humoral factors in these changes has not been determined. In deoxycorticosterone acetate (DOCA) salt hypertension, endothelin-1 (ET-1) peptide content and gene expression are enhanced in mesenteric resistance arteries. These vessels also present augmented vasoconstrictor responsiveness to vasopressin versus control uninephrectomized rats. To determine whether an interaction exists between vasopressin and ET-1 in the pathogenesis of small-artery structural alterations in DOCA-salt rats, we examined the effect of chronic V1 vasopressin receptor antagonism (OPC-21268, 30 mg/kg BID) on the structure and mechanical properties of mesenteric resistance arteries using a pressure myograph and the effect on preproendothelin-1 (preproET-1) gene expression, determined by Northern blot analysis of preproET-1 mRNA. Tail-cuff systolic pressures were elevated in DOCA-salt (200+/-11 mm Hg) versus uninephrectomized rats (109+/-4 mm Hg) and decreased slightly but significantly by OPC-21268 to 187+/-7 mm Hg (P<0.01). Treatment with DOCA-salt increased vascular media-lumen ratios and media cross-sectional areas and reduced both stress and incremental elastic modulus for a given pressure. However, there was no change in distensibility or incremental elastic modulus versus media stress. OPC-21268 partially attenuated the vascular growth in DOCA-salt rats. PreproET-1 mRNA was increased 2-fold in mesenteric arteries of DOCA-salt rats versus uninephrectomized rats, an effect abrogated by OPC-21268. Thus, DOCA-salt hypertension is associated with altered morphology of the small-arterial wall, without altering stiffness of the arterial wall components. OPC-21268 regressed in part these changes, suggesting the involvement of vasopressin. The concomitant attenuation of enhanced ET-1 expression by OPC-21268 suggests that ET-1 may be involved in mediating in part the vascular effects of vasopressin in DOCA-salt hypertensive rats.

Animals↗
PubMed1998

[Detecting coronary artery disease with dobutamine 99mTc-MIBI myocardial perfusion tomography].

OBJECTIVE: To assess the safety and accuracy of dobutamine stress 99mTc-MIBI perfusion tomography (DST) for detecting coronary artery disease(CAD). METHODS: Seventy nine patients with suspected CAD without previous myocardial infarction underwent DST. Forty eight of the 79 patients had coronary angiography and CAD was defined as greater than 50% reduction in diameter. RESULTS: Seventeen of 20 patients with CAD demonstrated perfusion abnormalities by DST (sensitivity 85%), 20 of 24 with normal or non-significant stenosed coronary arteries had normal scan (specificity 83.3%). All 4 patients with coronary ectasia showed abnormalities by DST. Fifty seven percent of the patients experienced some discomfort, but no serious side effects occurred. CONCLUSIONS: It shows that DST is a safe and accurate alternative to exercise stress perfusion study for detecting CAD in patients unable to perform exercise testing.

Adult↗
PubMed1998

[The relationship between different serological types of Pseudomonas aeruginosa strain in lower respiratory tract and its clinical significance].

OBJECTIVE: To explore the relationship between different serological types of pseudomonas aeruginose (PA) in sputum and its clinical significance. METHOD: PA was detected by Vitek-Ams GNI card and its sensitivity to 12 kinds of antibiotics was measured with GNS-GA, its different serological types were assayed with standard diagnostic serum of type 20. RESULT: 14 different serological types were isolated from 101 strains of PA in sputum (with the typed rate 84.2%). Among them, type 6, type 1, and type 3 were higher with 23.7%, 13.9% and 10.9% respectively. But another 15.8% of strains couldn't be differentiated, so called untyped PA group, among them 10 strains with mucoid-like colony (63% of this untyped group). Patients suffered with this group were older and were very seriously infected with higher mortality (13%). They often suffered from serious COPD and bronchiectasis. The sensitivities of all PA strains to antibiotics in vitro as follows: tobramycin 92.0%, ceftazidine 89.1%, ciprofloxacin 87.1%, ticarcillin 82.2%, and gentamycin 76.2%. Among 12 PA strains tested with imipenem sensitive test, only one strain of mucoid-like colony had been found resistant. CONCLUSION: Identification of serological type of PA can be used as an important method to make out if the infection comes from the same source, and for surveillance of hospital infection, and to show the main types of PA in some district. But the sensitivity of antibiotics in vitro is no definitive correlation with PA serological types. The disease infected with untyped PA strains have its clinically special characteristics and must be kept attention.

Adult↗
PubMed1998

[Chemical changes of compatibility for shaoyao decoction].

OBJECTIVE: To probe the regular patterns of interactions among chemical components in Chinese medicinal complex prescriptions. METHOD: The active components of Rhubarb, Coptis root and Baikal skullcap root in different patterns of compatibility were determined by TLC-scanner. RESULT: The berberine content in Coptis root can be decreased obviously by Rhubarb, Baikai skullcap root and Licorice root, especially by Rhubarb. Coptis root can also decrease the contents of acid components. CONCLUSION: The acid components can affect each other, thus reducing the contents of effective components.

Berberine↗
PubMed1998

[Dietary fiber protects intestinal structure and barrier function].

OBJECTIVE: To evaluate the effect of dietary fiber on intestinal structure and barrier function of 5-fluorouracil (5-Fu) challenged rat. METHOD: Thirty Wistar rats were divided randomly into three groups (10 each group): Chow, enteric nutrition (EN), and EN + Fiber group. Both EN and EN + Fiber group were isonitrogenic and isocaloric. The rats kept their diet respectively for 8 days. 5-Fu was injected intraperitoneally on day 4 postoperatively. Intestinal permeability (L/M) was measured respectively on day 3 and day 7. On day 8, the bacterial translation, wet weight and mucous thickness of both small intestine and colon and the villus height of small intestine were measured. The rats were weighted before and after experiment respectively. RESULT: The body weight loss of the EN + Fiber group (-3.1 +/- 3.4 g) was less than that of the EN group (-6.6 +/- 5.2 g) (P < 0.05), whereas the Chow group gained body weight (4.9 +/- 4.3 g) (P < 0.01 when compared with EN and EN + Fiber group). The parameters of intestinal structures of the EN + Fiber group was superior to the EN group (P < 0.05). The L/M of both EN + Fiber (from 0.0265 +/- 0.0073 to 0.0274 +/- 0.0068) and the Chow group (from 0.0268 +/- 0.0039 to 0.0281 +/- 0.0044) was unchanged (P > 0.05 for both) after 5-Fu challenged, whereas that of the EN group increased (from 0.0289 +/- 0.0070 to 0.0331 +/- 0.0084) (P < 0.01). The incidence of bacterial translocation to MLN of both EN + Fiber group and the Chow group (20%) was lower than that of the EN group (70%) (P < 0.05). CONCLUSION: The dietary fiber protects the intestinal structure and barrier function of 5-Fu challenged rats.

Animals↗
PubMed1998

Analysis of genetic diversity in Chinese sweetpotato [Ipomoea batatas (L.) Lam.] germplasm using DNA amplification fingerprinting.

The genetic diversity and evolutionary relationships in a representative sample of Chinese sweetpotato collection were assessed using the DNA amplification fingerprinting approach. DNA fingerprint profiles were developed for all 42 accessions tested. There were 19-26 bands for each accession and an average of 20.7 such bands were polymorphic. Chinese sweetpotato germplasm tested exhibited a high degree of genetic diversity. Phenetic analysis revealed five major clusters with the following components: (1) landraces from Guangdong Province, (2) landraces from Fujian province, (3) Chinese cultivars, (4) those closely related to Japanese sweetpotato cultivars, and (5) those closely related to the US sweetpotato cv. Nancy Hall. The genetic association observed between accessions was largely consistent with the known pedigree records. The DNA amplification fingerprinting may provide reliable insights into the domestication history of the sweetpotato crop and may be useful in germplasm enhancement.

Base Sequence↗
PubMed1998

CT findings in splenic tuberculosis.

Splenic tuberculosis is rare and delay in diagnosis is common. We present two cases of splenic tuberculosis with their appearance on CT. The CT findings were multiple, round or ovoid, low-density lesions without calcification. Except for non-specific lymphadenopathy no abnormality nor history suggestive of tuberculosis was revealed prior to laparotomy. Although CT does not confirm a diagnosis of splenic tuberculosis, it would be a valuable examination to define the extent of disease and guide aspiration biopsy.

Abscess↗
PubMed1997

Treatment with either anti-CD4 or anti-CD8 monoclonal antibodies blocks alphabeta T cell-mediated rejection of intestinal allografts in mice.

BACKGROUND: Rejection is the major barrier preventing the more widespread application of intestinal transplantation as treatment for intestinal failure. For this study, a one-way host-versus-graft murine model was used to investigate the contribution of T cell subsets to the rejection of allogeneic intestinal allografts. METHODS: Intestinal grafts consisting of the donor jejunum and ileum were procured from C57BL/6J (syngeneic group) and B6C3F1/J (C57BL/6 x C3H/HeJ, allogeneic group) mice. These grafts were then transplanted into (1) normal, (2) antibody-treated, or (3) genetically mutated C57BL/6 mice. Mice were killed at predetermined intervals and the grafts assessed for rejection by a blinded pathologist. RESULTS: No syngeneic mice demonstrated any evidence of rejection. In contrast, the recipients of allografts experienced progressive rejection. Recipient mice treated with tacrolimus developed significantly less severe allograft rejection. None of the alphabeta T cell-deficient recipient mice (T cell receptor beta chain knockout mice) experienced allograft rejection with follow-up ranging from 8 to 28 days. However, mice deficient in gammadelta T cells (T cell receptor delta chain knockout mice) rejected intestinal allografts in a manner indistinguishable from normal recipients. In order to investigate the role of CD4+ and CD8+ T cells, recipient mice were treated 2 days before transplantation with depleting monoclonal antibodies specific for either CD4+ cells or CD8+ cells. Depletion of either population of cells significantly inhibited allograft rejection. CONCLUSIONS: These data demonstrate that rejection of intestinal allografts in the murine model was absolutely dependent on alphabeta but not gammadelta T cells. Furthermore, both CD4+ and CD8+ T cells were necessary for small bowel allograft rejection. Additional studies will be required to determine whether the effects of monoclonal antibody treatment were due solely to depletion of T cells or were mediated at least in part through an active process that altered the functional properties of the targeted T cell subset.

Animals↗
PubMed1997

Studies on the comprehensive control of babesiasis in cattle and buffaloes.

To obtain a highly effective method for controlling babesiosis in cattle and buffalo, an epidemiological survey was carried out at Zuiyun village in Hubai Province. On the grounds of the epidemiological data, control measures which included preventive drugs and killing the vectors were devised. After two years of control no clinical cases of bovine babesiosis were found. The average number of ticks on cattle or buffalo decreased from 9-282 to 0-11.3. Good results were obtained in another epidemic area using this control method. These results indicated that this technique of controlling babesiosis is highly effective.

Animals↗
PubMed1997

[Effect of recombinant growth hormone on amino acids metabolism in blood and urine after major operation].

To determine the effects of recombinant human growth hormone (GH) on postoperative amino acids metabolism, we performed a placebo-controlled randomized double-blind trial in 18 patients after elective gastrectomy or colectomy. The same amount of calories and amino acids were administered. Research group was daily injected growth hormone 0.15 IU/(kg.day) and control group was given placebo during the first postoperative week. All urine were analyzed for nitrogen balance and excretion of 3-methylhistidine. On the seventh postoperative day while the parenteral nutrients were being infused, amino acid exchange across the forearm was measured. On the seventh postoperative day all patients' amino acids in plasma were slightly increased by comparing with preoperative day but was not statistical significance. Forearm arterial-venous difference (A-V) measured on POD + 7 was markedly different between groups. There was continued release of amino acids from the forearm in the control subjects, while there was marked amino acid uptake in the GH patients (total amino acid nitrogen exchange was -398 +/- 158 mumol/L vs +165 +/- 61 mumol/L, P < 0.01). Excretion of urinary 3-methylhistidine in control group loss more than in GH group (7.3 +/- 1.0 mumol/(kg.24 h) vs 4.4 +/- 0.3 mumol/(kg.24 h), P < 0.05). The results demonstrated that GH can increase the uptake of amino acid from forearm and decrease the excreation of 3-methylhistidine in urine after operation, but did not disturb the balance of amino acids in plasma.

Amino Acids, Branched-Chain↗
PubMed1997

[Histopathologic and immunochemical changes in Sjögren's syndrome].

To research into the causes of sjögren's syndrome, we investigated the lacrimal gland tissues of 6 cases of sjogren's syndriome by using electronmicroscopy and immunochemical methods. The results revealed that at the early stage the lacrimal gland cells showed degeneration, irregular arrangement and many intracellular vacuoles. At the middle stage, some lymphocytes infliltrated into the gland tissues. And at the advanced stage, lymphocytes and collagenous fibers were there in substitution of gland cells.

Adult↗
PubMed1997

[Extraction and sequencing of trace DNA from cornu Cervi pantotrichum].

The trace DNA extraction method was used to extract DNA from the blood, hair, Cornu cervi pantotrichum, hairy antler of the sika deer, Cervus nippon, and penis of Bulbalus bublis and Asinus vulgaris. A 307 bp fragment of mitochondrial DNA cytochrome b gene was amplified with primers L14841 and H15149 for these extractions. The purified PCR products were sequenced by the dideoxy method. DNA sequence obtained from the Cornu cervi pantotrichum is the same as that obtained from the fresh blood and hair of the sika deer, but the sequence of the so-called hairy antler is quite different, implying that it is not from the sika deer. In addition, the phylogenetic relationships among different species are congruent with the traditional known phylogeny.

Animals↗
PubMed1997

[The biological effects of recombinant human osteogenic protein-1 to cultured pulp cells].

The study was to observe the biological effects of rhOP-1 on the proliferation, ALPase activity and osteocalcin of dental pulp cells by cell culture technique, MTT, enzyme kinetic methods and radioimmunoassay. The results indicated rhOP-1 could obviously promote the proliferation and enhanced ALPase activity and osteocalcin secretion of pulp cells with showing dose-dependent relationship in this experimental dose range. Thus, rhOP-1 can induce dental pulp cells to differentiate the cells expressing osteoblast phenotype.

Alkaline Phosphatase↗
PubMed1997

[Pharmacological actions of different species form genus Pyrola].

This paper reporst the pharmacological actions and toxicities of the water extracts from Pyrola calliantha, Pyrola decorta and P. forrestiana. The results show that the actions of P. forrestiana on antiventricular fibrillation, strengthening cardiac musicular contraction and antihypoxia abitity are more stronger, but on antiinflammation and antipyretic, effects of P. decorta and P. calliantha are significantly better than that of P. forrestiana. The effects of the three species on immunization and antisepsis are similar.

Animals↗
PubMed1997

[Determination of metronidazole in gastric juice by high performance liquid chromatography].

Metronidazole (MTZ) is the first chosen drug of anti-anaerobe which was recommended by World Health Organization. In this paper a high performance liquid chromatographic (HPLC) method has been developed to determine the concertration of MTZ in human gastric juice. Gastric juice was obtained by intubation after taking drug, then the sample was precipitated with ethanol to remove proteins, centrifuged, filtered and finally assayed by HPLC. The operating conditions were reversed phase C18 column (4mm x 150mm), CH3OH:KH2PO4 (5mmol/L, pH 4.0) = 10:90 mobile phase with flow rate of 1.5mL/min and detection wavelength at 320nm. The standard working curve was Y = -1.63 + 1.15X, with r = 0.9999, n = 7. The linear range was 2.5-200mg/L. The detection limit was 0.4mg/L. The average recovery was 96.0% and the coefficient of variation was 3.07%. This method can be applied to guide clinical therapy and pharmaceutical research.

Adult↗
PubMed1997

Radioimmunoimaging of arterial and venous thrombi in canine model using 99mTc labeled monoclonal antifibrin antibody.

OBJECTIVE: To evaluate the usefulness of 99mTc labelled monoclonal antifibrin antibody SZ-63 for the detection of vascular thrombi, and to investigate the feasibility of thrombus imaging 99mTc-SZ-63. METHODS: The monoclonal antibody SZ-63 (Mab SZ-63), which reacts with D-dimer, XLFN and fibrin monomer was produced and labelled with 99mTc using pretinning procedure. After that, the femoral arterial or venous thrombus model was prepared in dogs and injected with 99mTc-SZ-63 intravenously. Both arterial and venous thrombus models were imaged. RESULTS: Both the arterial and venous thrombi were clearly visible within 2.5 hours after injection of 99mTc-SZ-63. Quantitative imaging analysis showed that the ratios between the thrombus and the opposite vessel increased with time extension. The ratios of thrombus to blood or surrounding muscle were 2. 56 +/- 0.83 and 13.40 +/- 5.10 for arterial thrombi and 3.34 +/- 1.06 and 22.40 +/- 6.40 for venous thrombi after the canines were killed. CONCLUSION: 99mTc-SZ-63 is a useful imaging agent for thrombus detection in vivo.

Animals↗
PubMed1997

[Studies on biological effects of kappa-selenocarrageenan on human breast cancer cell line BCaP-37].

Proliferation, cell cycle, total amount of DNA, area of cell nucleus, as well as epidermal growth factor receptors (EGFR) and expression of oncogene C-erbB2 mRNA of Chinese breast cancer cell line (BCaP-37) after being treated with kappa-selenocarrageenan were determined by cell culture technique, image cytometry (ICM) and northern blot to explore its anti-tumor mechanism. Results revealed 3.0-120 mg/L selenocarrageenan could inhibit proliferation of BCaP-37, with a response of time and dose dependence. The areas of nuclei were significantly lower with ICM in cells treated with 15 or 60 mg/L selenocarrageenan for four days than those in controls (P < 0.01). Levels of EGFR and expression of C-erbB2 mRNA were significantly inhibited in cells treated with 60 mg/L selenocarrageenan. It suggests that selenocarrageenan can inhibit proliferation of breast cancer cells through regulation of the levels of EGFR and expression of C-erbB2 mRNA.

Antineoplastic Agents↗